The Polarographic Reduction of Osmium Tetroxide

Crowell, William R.; Heyrovský, J.; Engelkemeir, D.W.

doi:10.1021/ja01856a009

Cited by 23 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2b, c). This peak is caused by the reversible redox couple osmium (IV/VI) according to earlier findings of Lukasova et al [18], as well as results of Crowell et al and Meites [41,42]. The reduction of Os(VIII) happens at 0 V and the reduction of Os(IV) to Os(III) at ca.…”

Section: Modification Of Fragment Oligonucleotide (Target)supporting

confidence: 76%

Electrochemical Detection of Asymmetric PCR Products by Labeling with Osmium Tetroxide

et al. 2009

View full text Add to dashboard Cite

Single stranded DNA-targets from asymmetric polymerase chain reaction (PCR) of a sequence of the gram positive, spore forming bacterium Clostridium acetobutylicum were detected by square-wave voltammetry after labeling with osmium tetroxide bipyridine and hybridization with DNA capture probes immobilized on gold electrodes. The asymmetric PCR, performed with a 10-fold excess of the forward-primer, was used without any further purification for hybridization with protective strands and covalent labeling with osmium tetroxide bipyridine. Square-wave voltammetric signals of 20 nmol/L targets were significantly higher at 50 8C compared with 23 8C hybridization temperature. A fully noncomplementary protective strand yielded thoroughly modified targets unable for further hybridization. Coupling this with thermal discrimination opens new opportunities for sequence specific DNA detection.

show abstract

Section: Modification Of Fragment Oligonucleotide (Target)supporting

confidence: 76%

Electrochemical Detection of Asymmetric PCR Products by Labeling with Osmium Tetroxide

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Our results also confirm that Os(VIII)bipy modifies only pyrimidine bases, while reactivity of Os(VI)-bipy is limited to the ribose residue in ribosides. In both sugar-modified and base-modified ribosides the overall electrode process may follow a path similar to osmium tetroxide electroreduction studied earlier by J. Heyrovský [41] and L. Meites [42] but not including the reduction of Os(VIII), because in both the sugar-and base-modified ribosides six-valent Os is present (Fig. 1).…”

Section: Electrochemistry Of Sugar-modified and Basemodified Nucleosidesmentioning

confidence: 63%

Covalent Labeling of Nucleosides with VIII‐ and VI‐Valent Osmium Complexes

et al. 2007

View full text Add to dashboard Cite

Osmium tetroxide complexes with nitrogen ligands [Os(VIII)L] have been widely applied as probes of the DNA structure and as electroactive labels of DNA. Here we describe the electrochemical behavior of Os(VIII)2,2-bipyridine (Os, bipy)-base-labeled nucleosides. We show that electroactive label can be introduced also in the nucleoside ribose residues using six-valent osmium complex. Cyclic voltammograms of sugar-Os(VI)-modified ribosides are similar but not identical to those of the base-modified ribosides. Our results showing the electroactivity of sugar modified ribosides pave the way to facile end-labeling of RNA.

show abstract

“…With respect to osmium, there are also some contributions that describe the use of classical polarography, voltammetry or coulometry for such purposes. One of the very first reports is a work by Crowell et al focused on the polarographic reduction of the osmium tetroxide, OsO 4 , in highly alkaline media [6]. Similar studies with octavalent osmium have been described by Cover, Connery, and Meites [7 -9], who had experimented with a series of Os(VIII) compounds in various media, over the whole pH-range, in the presence of some inorganic and organic complex-forming substances.…”

Section: Introductionmentioning

confidence: 83%

A Study on Stripping Voltammetric Determination of Osmium(IV) at a Carbon Paste Electrode Modified In Situ with Cationic Surfactants

et al. 2006

View full text Add to dashboard Cite

This article deals with the development of a method for the determination of osmium at a carbon paste electrode (CPE) modified with cationic surfactants of the quaternary ammonium salt type; namely, cetyltrimethylammonium bromide (CTAB) and 1-(ethoxycarbonyl)-pentadecyltrimethyl-ammonium bromide (Septonex); both being added in situ and serving for preconcentration of osmium via its hexachloroosmate(IV) anion. The proper electrochemical detection was performed by cathodic scanning in the differential pulse voltammetric mode. Optimization studies concerning important experimental parameters also included a specially performed potentiometric titration, helping to define the actual stoichiometry for the ion-pairing process, the main principle and driving force of the accumulation step. In a chloride/acetate buffer based supporting medium and with Septonex as the modifier of choice, the reduction signal for osmium was found to be proportional to the Os(IV) concentration in a range from 5 Â 10 À9 to 5 Â 10 À7 mol L À1 with a limit of detection close to 5 Â 10 À9 mol L À1 (with preconcentration for 60 s). The method capable to determine Os(IV) in the presence of both Pt(IV) and Ir(III) was tested on model solutions as well as with real sample of industrial waste water (spiked with the analyte); both yielding the recovery rates within 88 -99%.

show abstract

The Polarographic Reduction of Osmium Tetroxide

Cited by 23 publications

References 0 publications

Electrochemical Detection of Asymmetric PCR Products by Labeling with Osmium Tetroxide

Electrochemical Detection of Asymmetric PCR Products by Labeling with Osmium Tetroxide

Covalent Labeling of Nucleosides with VIII‐ and VI‐Valent Osmium Complexes

A Study on Stripping Voltammetric Determination of Osmium(IV) at a Carbon Paste Electrode Modified In Situ with Cationic Surfactants

Contact Info

Product

Resources

About