The Pollen Coat Proteome: At the Cutting Edge of Plant Reproduction

Rejón, Juan David; Delalande, François; Schaeffer-Reiss, Christine; Alché, Juan de Dios; Rodríguez-García, Marìa Isabel; Dorsselaer, Alain Van; Castro, Antonio Jesús

doi:10.3390/proteomes4010005

Cited by 45 publications

(43 citation statements)

References 122 publications

(218 reference statements)

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“…caleosins and acetyl cholinesterases), and pollen tube growth (e.g. expansins and glucanases; Rejón et al, 2016). Tryphine is deposited in the cavities of the outer exine to form the pollen coat.…”

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confidence: 99%

A MYB Triad Controls Primary and Phenylpropanoid Metabolites for Pollen Coat Patterning

et al. 2019

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The pollen wall is a complex, durable structure essential for plant reproduction. A substantial portion of phenylpropanoids (e.g. flavonols) produced by pollen grain tapetal cells are deposited in the pollen wall. Transcriptional regulation of pollen wall formation has been studied extensively, and a specific regulatory mechanism for Arabidopsis (Arabidopsis thaliana) pollen flavonol biosynthesis has been postulated. Here, metabolome and transcriptome analyses of anthers from mutant and overexpression genotypes revealed that Arabidopsis MYB99, a putative ortholog of the petunia (Petunia hybrida) floral scent regulator ODORANT1 (ODO1), controls the exclusive production of tapetum diglycosylated flavonols and hydroxycinnamic acid amides. We discovered that MYB99 acts in a regulatory triad with MYB21 and MYB24, orthologs of emission of benzenoids I and II, which together with ODO1 coregulate petunia scent biosynthesis genes. Furthermore, promoter-activation assays showed that MYB99 directs precursor supply from the Calvin cycle and oxidative pentose-phosphate pathway in primary metabolism to phenylpropanoid biosynthesis by controlling TRANSKETOLASE2 expression. We provide a model depicting the relationship between the Arabidopsis MYB triad and structural genes from primary and phenylpropanoid metabolism and compare this mechanism with petunia scent control. The discovery of orthologous protein triads producing related secondary metabolites suggests that analogous regulatory modules exist in other plants and act to regulate various branches of the intricate phenylpropanoid pathway.

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confidence: 99%

A MYB Triad Controls Primary and Phenylpropanoid Metabolites for Pollen Coat Patterning

et al. 2019

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“…We followed the conventional fixing procedure, which resulted in some pollen rupture as some nuclei were seen outside the pollen wall. Plant pollen walls harbor some allergens, as reviewed recently [26], and our research can add defensin-like protein to the list.…”

Section: Discussionmentioning

confidence: 99%

Localization of Four Allergens in Artemisia Pollen by Immunofluorescent Antibodies

Zhao

Gao

et al. 2019

Int Arch Allergy Immunol

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Background: Artemisia pollens have a high potential to induce allergic symptoms. Seven allergen components have been identified, but only Art v 7 has been localized in the pollen grain. This study aimed to localize the allergens in the pollen grains of 4 Artemisia spp. Methods: Pollen extracts from 2 Chinese Artemisia spp., A. argyi and A. annua, were used to immunize BALB/c mice. Recombinant Art v 1 and Art v 3 allergens were used to select specific monoclonal antibodies (mAbs). Three mAbs were used to purify the natural allergens and were then analyzed by mass spectrometry. As reported previously, polyclonal antibodies were obtained from rabbits immunized with 3 synthesized peptides of Art an 7. Using conventional histology procedures with pollens from 4 Artemisia spp. (A. argyi, A. annua, A. capilaris, and A. sieversiana), allergen images were observed and recorded by fluorescence and confocal laser microscopy. Results: We obtained 2 specific mAbs against Art v 1, 1 against Art v 2, and 4 against Art v 3 homologs. The Art v 1 and Art v 3 homologs were mainly located on the pollen walls, and the Art v 7 homologous protein was localized intracellularly around nuclei. The location of the Art v 2 homologous protein varied across species, being intracellular around nuclei for A. annua and A. argyi, and in both the pollen wall and around nuclei for A. capilaris and A. sieversiana. Conclusions: Four mugwort allergens were localized in the pollen, and the major Art v 1 and Art v 3 allergens were located mainly in the pollen wall.

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“…The intine is an inner layer comprised of pectin and cellulose, while the exine is composed of a biopolymer complex, called sporopollenin, consisting of fatty acids, carotenoids, and phenylpropanoids provided by the tapetum (Heslop-Harrison, 1971;Quilichini et al, 2014). Sporopollenin provides the pollen grain with a tough outer layer and assists in adhesion to stigmatic surfaces to initiate pollenstigma interaction and plant reproduction (Rejón et al, 2016;Zinkl et al, 1999). Later in anther development, the tapetum degenerates via programmed cell death, tapetal debris fills the anther locule and some material (e.g.…”

Section: Arabidopsis Thaliana Reproductionmentioning

confidence: 99%

“…Picture was taken by Ms. Denise Chabot. light, and helps during pollination and pollen-stigma interactions (Rejón et al, 2016). At anther maturity, it dehisces and releases the pollen.…”

Section: Figure 2 Transmission Electron Microscopy Of Arabidopsis Thmentioning

confidence: 99%

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Characterization of an Arabidopsis thaliana Anther-Specific Caleosin

Vali¹

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Anther and pollen development are important to plant reproduction processes leading to seed production. The floral bud of the model plant organism, Arabidopsis thaliana, consists of 6 anthers which encase copious amounts of pollen. Within the anther locules, a variety of nutrients are provided to pollen in order to support its development. This study aims to offer a deeper understanding of the role of anther caleosins in the Brassicaceae by investigating the expression, localization, and putative function of the anther-specific caleosin (AT1G23240). Through the use of various molecular and microscopy techniques (e.g., quantitative reverse transcriptase polymerase chain reaction, Western blot analysis, laser confocal microscopy, and transmission electron microscopy), it was determined that AT1G23240 was expressed at late stages of anther development -mainly within the endoplasmic reticulum and tapetosomes of tapetal cells, and the protein was also detected on the pollen coat of mature pollen. This elucidation of anther caleosin expression will help in better understanding its role and significance in the overall plant reproduction process, possibly in pollen-stigma interactions, as well as its potential in biotechnological applications in regards to fertility within key crop kinds of the Brassicaceae family. PREFACEWithin this thesis, techniques such as the qualitative polymerase chain reaction, Western blot analysis, confocal laser scanning microscopy, and transmission electron microscopy were used to elucidate the developmental expression, localization, and potential function of the Arabidopsis thaliana anther-specific caleosin AT1G23240. Figures and text used within this thesis are intended for later submission and publication of my findings.While I carried out the majority of the work within this thesis, I would like to thank the

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The Pollen Coat Proteome: At the Cutting Edge of Plant Reproduction

Cited by 45 publications

References 122 publications

A MYB Triad Controls Primary and Phenylpropanoid Metabolites for Pollen Coat Patterning

A MYB Triad Controls Primary and Phenylpropanoid Metabolites for Pollen Coat Patterning

Localization of Four Allergens in Artemisia Pollen by Immunofluorescent Antibodies

Characterization of an Arabidopsis thaliana Anther-Specific Caleosin

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