The polymerase chain reaction

Powledge, Tabitha M.

doi:10.1152/advan.00002.2004

Cited by 44 publications

(25 citation statements)

References 1 publication

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“…It can also be implemented for detection of AdV serotypes which are potentially considered as not growing in routine cell culture. Moreover, polymerase chain reaction may be employed for detection of variance and mutations in virus genome (Powledge, 2004). In combination with other methods such as sequencing of hyper variation regions (HVRs) or restriction fragment length analysis, PCR may be used for AdV typing.…”

Section: Diagnostic Methods For Adenovirus Detection and Identificationmentioning

confidence: 99%

Adenoviral Infection – Common Complication Following Hematopoietic Stem Cell Transplantation

Bil‐Lula¹,

Ussowicz²,

Woźniak³

2012

New Advances in Stem Cell Transplantation

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Section: Diagnostic Methods For Adenovirus Detection and Identificationmentioning

confidence: 99%

Adenoviral Infection – Common Complication Following Hematopoietic Stem Cell Transplantation

Bil‐Lula¹,

Ussowicz²,

Woźniak³

2012

New Advances in Stem Cell Transplantation

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“…The advent of PCR generated high hopes among those in search of history in the bases of aDNA. Even today, archeologists-like those seeking information on the Dead Sea Scrolls or on the human brains discovered in a Florida sinkhole [28]-use PCR for its convenience, low cost, and the ease with specific sequences which can be recovered from the same sample a number of times [29]. Despite the advantages, PCR presents drawbacks that perturb its reliability in aDNA analysis.…”

Section: Dna Detection: the Classical Techniquesmentioning

confidence: 99%

Raman Spectroscopy: A Promising Technique for Analyzing Nucleic Acid Refractory to PCR Amplification

Matar¹,

Merheb²

2014

TOSPECJ

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Ancient DNA (aDNA) refers to the genetic material found in dead paleontological and archeological samples. Being subject to various types of stressors, it undergoes different hydrolytic and oxidative post mortem modifications that result in the formation of DNA lesions. These lesions are found to either block the DNA polymerase during replication or induce nucleotide misincorporations. Besides, aDNA samples occur in minimal quantities; which represents an additional obstacle that researchers have to overcome in order to study aDNA. The earliest major efforts included the use of molecular cloning and polymerase chain reaction (PCR) to amplify aDNA sequences. These techniques were later found to be associated with a number of false results and unauthentic findings. There have been numerous attempts to eliminate the shortcomings of PCR and improve the quality of aDNA through avoiding contamination, repairing lesions, using translesion polymerases, etc. However, the majority of these have failed to yield accurate and specific results. Surfaceenhanced resonance Raman scattering (SERRS), on the other hand, starts in the right foot by introducing a sensitive nonenzymatic approach for the specific detection of single-and double-stranded DNA. The ability of this method to evade DNA degradation is particularly important for not only studying aDNA, but also analyzing DNA refractory to PCR amplification in processed products.

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“…PCR can be extensively modified, and has a wide range of applications [28,29]. We describe here some usually applied PCR techniques to diagnose EGFR mutations in NSCLC.…”

Section: Reviewmentioning

confidence: 99%

Evaluation of current methods to detect the mutations of epidermal growth factor receptor in non-small cell lung cancer patients

Obradovic

Jurišić

2012

Multidiscip Respir Med

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Many different methods were developed to detect commonly known mutations and to screen new mutations of the epidermal growth factor receptor in non-small cell lung cancer patients. Some of these methods are so sensitive as to be able to detect even one epidermal growth factor receptor mutant tumor cell among up to 1000–2000 normal cells. We have considered current methods chronologically reported to detect mutations in epidermal growth factor receptor in patients with non-small cell lung cancer. We also gave a short preview of their significance for routine clinical works. A Pub Med literature search was performed in order to demonstrate what methods are mostly used in mutation detection and to show their distribution through the last 10 years.

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The polymerase chain reaction

Cited by 44 publications

References 1 publication

Adenoviral Infection – Common Complication Following Hematopoietic Stem Cell Transplantation

Adenoviral Infection – Common Complication Following Hematopoietic Stem Cell Transplantation

Raman Spectroscopy: A Promising Technique for Analyzing Nucleic Acid Refractory to PCR Amplification

Evaluation of current methods to detect the mutations of epidermal growth factor receptor in non-small cell lung cancer patients

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