The Polymerase Chain Reaction and Plant Disease Diagnosis

Henson, J

doi:10.1146/annurev.phyto.31.1.81

Cited by 122 publications

(145 citation statements)

References 26 publications

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“…Techniques based on polymerase chain reaction (PCR) and nucleic acid hybridization were used for the detection of these viruses [37]. PCR allows the detection of very small amounts of plant viruses in their hosts and vectors [19,21,22]. As PCR amplifies the viral nucleic acid, this approach is extremely useful in bypassing problems associated with serology.…”

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confidence: 99%

Detection of Corchorus golden mosaic virus Associated with Yellow Mosaic Disease of Jute (Corchorus capsularis)

et al. 2012

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Yellow mosaic disease, caused by a whitefly transmitted New World Begomovirus, named Corchorus golden mosaic virus (CoGMV), is emerging as a serious biotic constraint for jute fibre production in Asia. For rapid and sensitive diagnosis of the Begomovirus associated with this disease, a non-radiolabelled diagnostic probe, developed against the DNA A component of the east Indian isolate of CoGMV, detected the presence of the virus in infected plants and viruliferous whiteflies following Southern hybridization and nucleic acid spot hybridization tests. Presence of the virus was also confirmed when polymerase chain reaction amplification was performed using virus-specific primers on DNA templates isolated from infected plants and viruliferous whiteflies.

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confidence: 99%

Detection of Corchorus golden mosaic virus Associated with Yellow Mosaic Disease of Jute (Corchorus capsularis)

et al. 2012

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“…Since its development, the random-amplifi ed polymorphic DNA (RAPD) protocol has acquired a diversity of uses, such as: the establishment of the genetic similarity degree between individuals within a population [22], the construction of genetic maps as well as the localization of economically interesting genes [23], the production of a genomic fi ngerprint [24], and the study of genetic diversity along with the identifi cation of fungi [25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Morphological and genetic differentiation among four pigment producing Indian species of Phoma (Saccardo, 1899)

Chande

Kövics

Sandhu³

et al. 2010

Indian J Microbiol

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A PCR-based technique, involving the random amplifi cation of polymorphic DNA (RAPD), was used for assessing genetic relatedness among isolates of the genus Phoma. Randomly Amplifi ed Polymorphic DNA (RAPD) revealed the presence of interspecifi c genetic variation among the pigment producing isolates of Phoma and has shown distinct phylogenetic cluster. The major objective of the study was to study the genetic variation, if any. Study was aimed to differentiate four pigment producing species of Phoma based on morphological studies and molecular markers in general and RAPD in particular. We found that the test species of Phoma can be very well differentiated using molecular markers. Phoma sorghina was differentiated from P. exigua, P. fi meti and P. herbarum. RAPD profi les of P. herbarum and P. fi meti has shown the maximum similarity, which indicates the genetic relatedness among these two species which were considered earlier as distinct species based on morphological observation.

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“…This is probably the reason why it has not been detected yet on seeds in Argentina (Barreto & Carmona, 1993). An assay carried out using polymerase chain reaction (PCR) allowed the detection of small concentrations of a specific DNA in a complex environment (Henson & French, 1993). PCR is highly sensitive…”

Section: Introductionmentioning

confidence: 99%

Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique

Ríos

Fernández²,

Carmona

2007

Fitopatol. bras.

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Leaf scald of barley caused by Rhynchosporium secalis is an important disease in Argentina. The fungus is a necrotrophic pathogen which survives in stubble, seeds and weeds. Isolation of R. secalis from seeds on artificial media usually has not been successful due to the slow growth rate of the pathogen and strong inhibition by contaminants. The objective in this work was to detect R. secalis in different genotypes of barley seeds in Argentina using the polymerase chain reaction (PCR)-based diagnostic assay. Four barley genotypes were tested in 2004: Quilmes Ayelén, Quilmes Alfa, Barke and Maltería Pampa 1004. The previously described RS8 and RS9 primers were used for the detection of R. secalis in barley seeds. A 264-bp single band was obtained for each cultivar showing the presence of R. secalis. The use of specific primers was efficient in the detection of R. secalis in barley seeds in Argentina and could be used for routine diagnosis, epidemiology and seed transmission studies. This is the first report on the detection of R. secalis in barley seeds in Argentina.

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The Polymerase Chain Reaction and Plant Disease Diagnosis

Cited by 122 publications

References 26 publications

Detection of Corchorus golden mosaic virus Associated with Yellow Mosaic Disease of Jute (Corchorus capsularis)

Detection of Corchorus golden mosaic virus Associated with Yellow Mosaic Disease of Jute (Corchorus capsularis)

Morphological and genetic differentiation among four pigment producing Indian species of Phoma (Saccardo, 1899)

Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique

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