The polymorphism of Insulin-like growth factor-I (IGF-I) is related to osteoporosis and bone mineral density in postmenopausal population

Abstract

Objective: It has been shown that Insulin-like growth factor-1 (IGF-1) may be related with bone mineral density (BMD) or osteoporosis. But there are few evidences on the role of genetic variation of IGF-1 on the BMD or osteoporosis. We observed the relationship between polymorphisms of IGF-1(rs35767, rs2288377 and rs5742612) with osteoporosis and BMD in the postmenopausal female population in our study. Methods: A total of 216 postmenopausal women with a primary diagnosis of osteoporosis and 220 normal healthy women were included in the study. Genomic DNA of IGF-1 rs35767, rs2288377 and rs5742612 was extracted from the whole blood using QIAamp blood DNA mini kits (QIAGEN, Hilden, Germany) according to the methods recommended by the manufacturer. Results: We found that T allele of rs35767 had higher increased risk of osteoporosis (OR=1.34, 95%CI=1.0-1.81). Those carrying T allele of rs35767 had a significant lower BMD at L1–L4 vertebrae, femoral neck, total hip and trochanter when compared with those carrying C allele (P < 0.05). In addition, the BMD of L1–L4 vertebrae, femoral neck, total hip and trochanter decreased by 2.09%, 3.74%, 3.52% and 2.54% in women carrying T alleles compared with those carrying C alleles. Conclusion: Our study suggests that polymorphism in IGF-I rs35767 was significantly associated with BMD and osteoporosis in postmenopausal female population, and polymorphism of rs35767 could be a marker for lower BMD and risk of osteoporosis.

Association of the insulin-like growth factor-1 single nucleotide polymorphisms rs35767, rs2288377, and rs5742612 with osteoporosis risk

“…[ 4 ] Among the included studies, the study by Yun-kai demonstrated that carriers of the rs35767 T allele had lower values of BMD at L1-L4 vertebrae, femoral neck, total hip, and trochanter than those carrying the C allele. [ 23 ] Similar findings were obtained from the study by Zhang et al, which showed that BMD levels at L1-L4 vertebrae, femoral neck, total hip, and trochanter were significantly decreased in the CT + TT genotypes of rs35767 in comparison with the CC genotypes in osteoporosis patients. [ 25 ] It was of interest to evaluate the association between rs35767 and BMD.…”

“…Among these, 4 case-control studies met the inclusion criteria and were included in the final analyses. [ 23 – 26 ] Figure 1 summarizes the process of identifying eligible studies. All of the studies were published in English.…”

“…A total of 2807 participants took part in the studies, with 1 recruiting men and women, [ 25 ] and 3 women only. [ 23 , 24 , 26 ] Four studies investigated rs35767, [ 23 – 26 ] 3 studies assessed rs2288377, [ 23 – 25 ] and 3 studies evaluated rs5742612. [ 23 – 25 ] The pooled minor allele frequency for rs35767, rs2288377, rs5742612 was 0.291 (95% CI: 0.275–0.308), 0.100 (95% CI: 0.088–0.113), and 0.131 (95% CI: 0.118–0.146), respectively.…”

“…However, there was discrepancy between the 2 studies in the selection of cases and controls for evaluating BMD. Yun-kai et al divided subjects according to allele status, [ 23 ] whereas the study by Zhang et al utilized genotypes. [ 25 ] Due to discrepancy in study design and limited data availability (n < 3), we did not perform subgroup analysis for BMD.…”

Associations of IDUA and PTCH1 with Bone Mineral Density, Bone Turnover Markers, and Fractures in Chinese Elderly Patients with Osteoporosis

“…The etiology of SOP is mainly influenced by environmental and genetic factors [18]. Numerous GWAS and candidate gene studies have demonstrated that genetic variances in the vitamin D receptor ( VDR ) gene [19, 20], lipoprotein receptor-related protein 5 ( LRP5 ) gene [21–24], and Insulin-like Growth Factor-1 ( IGF-1 ) gene [25–27] are involved in the regulation of bone remodeling and affect bone mass. In addition, the IDUA gene and the PTCH1 gene have been a research focus on the relation of SNPs with BMD and OP in recent years.…”