The importance and significance of the conservation of the Emerald Object “The lower part of the Uda River Valley” (UA0000295) in botanical and general ecological aspects is discussed in detail. It is located on the outskirts of Kharkiv with an area of 13,381.0 ha. The studied area combines floodplains of regions occupied by meadows, shrub fragments and forest vegetation, and pine forests with artificial pine plantations. Areas of the loess plateau with arable land and deciduous forests, as well. List of the leading plant species, area's, main ecological and biotic features of identified habitats under Resolution 4 of the Berne Convention, in particular, C1.222, C1.32, C1.33, C3.34, D5.2, E1.2, E2.2, E3.4, F9.1, F3.247, G1.11, G1.21, G1.41, G1.8, G3.4232, G1.A4, G1.A1 for the Emerald Object are presented. The largest areas are occupied by biotops of lowland bogs with sedge and reed thickets without stagnant water (D5.2). There are plain hay fields (E2.2) and wet and moist meadows with a predominance of grasslands (E3.4) and deciduous forests in medium rich and rich soils (G1.A1), fresh and dry forests, and Sarmatian-type forests (G3.4232). The typological diversity of the forest of the territory, the area covered with forest vegetation is 11,585.0 ha, were outlined. Oak (Quercus robur L.) (8,091.5 ha, 69.83%) with a predominance in its plantations of fresh maple-linden oak and Scots pine (Pinus sylvestris L.) (2,529.8 ha, 21.84%) with a predominance of fresh oak-pine sedge are the main forest-forming species. Some species have appropriate conservation status in Ukraine (Botrychium lunaria (L.) SW., Dactylorhiza incarnata (L.) Soo s.l., Epipactis palustris (L.) Crantz, Anacamptis coriophora (L.) R.M. Bateman, Pridgeon & M.W. Chase (Orchis coriophora L.), A. palustris (Jacq.) R.M. Bateman, Pridgeon & M.W. Chase (Orchis palustris Jacq.), and several species are protected at the regional level (Centaurium erythraea Rafn., C. pulchellum (Sw.) Druce, Dianthus stenocalyx Juz., Inula helenium L., Iris pseudacorus L., Filipendula ulmaria (L.) Maxim., Caltha palustris L., Geum rivale L., Parnassia palustris L., Sanguisorba officinalis L., Valeriana officinalis L.)