The population structure of Hemileia vastatrix in Brazil inferred from AFLP

Maia, Thiago; Maciel-Zambolim, E.; Caixeta, Eveline Teixeira; Mizubuti, Eduardo S. G.; Zambolim, Laércio

doi:10.1007/s13313-013-0213-3

Cited by 26 publications

(39 citation statements)

References 29 publications

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“…However, the genetic diversity was relatively lower than the diversity found in the present study. However, Maia et al (2013) (also in Brazil) obtained results similar to those of the present study using 91 fungal isolates; they proposed that H. vastatrix acts as a large indifferentiated population with high genotypic diversity that is not structured based on its geographic origin and host. Therefore, the lack of structure of the two H. vastatrix populations by region may be explained by the fact that the fungus is spread over long distances.…”

Section: Resultados Y Discusiones Análisis De La Diversidad Genética supporting

confidence: 85%

“…Estos resultados son similares a los obtenidos por Cabral et al (2016) en Brasil, quienes no observan una estructuración de la población de H. vastatrix respecto a su origen, hospedero ni razas fisiológicas; sin embargo, la diversidad genética fue relativamente menor a la hallada en el presente trabajo. No obstante, Maia et al (2013) también en Brasil, obtuvieron resultados similares al presente trabajo, a través del estudio de 91 aislados del hongo, proponiendo que H. vastatrix se comporta como una gran población populations were structured based on their origin, host or physiological race. However, the genetic diversity was relatively lower than the diversity found in the present study.…”

Section: Resultados Y Discusiones Análisis De La Diversidad Genética unclassified

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Diversidad genética de Hemileia vastatrix de dos Zonas productoras de café en el Perú

Quispe-Apaza

Mansilla

LÃ3pez-Bonilla

et al. 2017

RMF

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Se analizó la diversidad genética del patógeno del café Hemileia vastatrix, a través de secuenciación de los espaciadores internos transcribibles del ADN ribosomal (ITS). El análisis se realizó en muestras de roya de 12 predios de dos zonas cafetaleras del Perú, Quillabamba (Cusco) y Villa Rica (Pasco). Se hallaron los índices de diversidad haplotípica y nucleotídica. Además, se determinaron las semejanzas entre las poblaciones analizadas por medio de una red de haplotipos. Del análisis, se determinó que ambas regiones productoras presentan valores altos de diversidad genética; sin embargo, la zona de Quillabamba albergó la mayor diversidad haplotípica de H. vastatrix (Hd = 0.977 +/- 0.012). Asimismo, la red de haplotipos permitió evidenciar la estructura de las poblaciones de H. vastatrix para cada zona, las que juntas se comportan como una gran población indiferenciada con presencia de haplotipos ancestrales a partir de los cuales se fueron generando nuevas variantes del hongo. También, se analizaron las secuencias de la región ITS de H. vastatrix peruanas y las almacenadas en el GenBank, no encontrándose distribución de las poblaciones por región geográfica; además, se determinó que las poblaciones de H. vastatrix peruanas presentan haplotipos similares a los de Colombia y a las razas II y XXII.

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Section: Resultados Y Discusiones Análisis De La Diversidad Genética supporting

confidence: 85%

Section: Resultados Y Discusiones Análisis De La Diversidad Genética unclassified

Diversidad genética de Hemileia vastatrix de dos Zonas productoras de café en el Perú

Quispe-Apaza

Mansilla

LÃ3pez-Bonilla

et al. 2017

RMF

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“…The results showed low values of Nei's gene diversity ( h = 0·027 ± 0·064), in contrast with those obtained by Maia et al . () and Nunes et al . (), who found values seven ( h = 0·194 ± 0·130) and 10 times higher ( h = 0·280 ± 0·150), respectively.…”

Section: Discussionmentioning

confidence: 86%

“…(), all isolates evaluated were from single lesions (genetic uniform material), whereas the methods of Maia et al . () and Nunes et al . () consisted of bulk isolates.…”

Section: Discussionmentioning

confidence: 86%

Genetic diversity and structure of Hemileia vastatrix populations on Coffea spp.

et al. 2015

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Coffee leaf rust is the most limiting disease for coffee cultivation in Brazil. Despite its importance, relatively little is known about the genetic diversity of Hemileia vastatrix, the rust causal agent. In this work, the DNA from 112 monopustule isolates from different geographic locations and coffee genotypes were analysed by amplified fragment length polymorphisms (AFLP). The objectives were to assess the influence of the host and geographic origin on the diversity and population differentiation in H. vastatrix. The fungal population showed a low level of genotypic diversity. Gene diversity (h) was 0·027 and the hypothesis of random mating in the total population was rejected, but evidence for recombination was found for two subpopulations (São Paulo and Paraná). The analysis of molecular variance revealed that 90% of the genetic distribution of the pathogen occurs among isolates within the subpopulation (states or host of origin). There was no correlation between geographic and genetic distance (r = −0·024, P = 0·74), which together with the high number of migrants and the low degree of differentiation in populations of H. vastatrix, is consistent with the fact that the inoculum is probably easily dispersed by wind over long distances, allowing dispersal of the pathogen among coffee growing areas in Brazil. Therefore, it is difficult to predict the durability of resistant sources to coffee rust. The recommendation for the breeding programmes is thus to incorporate multigenic resistance as a control strategy.

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“…Gouveia, Ribeiro, Várzea, and Rodrigues () analysed the genetic diversity of H. vastatrix using RAPD markers and observed moderately low genetic diversity among the population. In another study, Maia, Maciel‐Zambolim, Caixeta, Mizubuti, and Zambolim () analysed the population structure of H. vastatrix from Brazil and observed high level of genotypic diversity using AFLP marker. In Brazil, Cabral, Zambolim, Oliveira, and Zambolim () studied the influence of host and geographic origin on the genetic diversity and population structure of H. vastatrix using AFLP markers and suggested that the fungal population revealed low levels of genotypic diversity.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity of coffee leaf rust pathogen Hemileia vastatrix using SRAP markers

Kosaraju¹,

Sannasi

Mishra³

et al. 2017

Journal of Phytopathology

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Coffee leaf rust caused by the fungus Hemileia vastatrix (Berk and Br.) is a major disease occurring in coffee plantations. Although the rust fungus exists in different physiological races, the genetic difference between them is meagrely understood. In this study, genetic diversity of 14 identified and two unidentified leaf rust races was determined by sequence‐related amplified polymorphism (SRAP) markers. Of 48 SRAP primer pairs tested, 35 primers are polymorphic and generated 347 distinct scorable fragments. The number of fragments ranged from 4 to 18 with a mean of 9.97 fragments per primer combination. Of the total 347 amplified fragments, 185 fragments (53.31%) are polymorphic with an average of 5.41 fragments per primer combination. The average resolving power (Rp) and the average polymorphism information content (PIC) of the 35 SRAP primer combinations were 13.60 and 0.356, respectively. Of 35 SRAP primer pairs, 15 primer pairs were more informative and generated 25 unique fragments, which are useful for race discrimination. The study demonstrated the existence of genetic variability among various leaf rust races and this information will be helpful in coffee breeding programmes.

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The population structure of Hemileia vastatrix in Brazil inferred from AFLP

Cited by 26 publications

References 29 publications

Diversidad genética de Hemileia vastatrix de dos Zonas productoras de café en el Perú

Diversidad genética de Hemileia vastatrix de dos Zonas productoras de café en el Perú

Genetic diversity and structure of Hemileia vastatrix populations on Coffea spp.

Assessment of genetic diversity of coffee leaf rust pathogen Hemileia vastatrix using SRAP markers

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