Pulsed laser range-gated imaging (PLRGI) is one of the most effective methods to achieve underwater high-resolution imaging. When searching target, there are two methods can be used: fixed gate and sliding gate. In practice, fixed gate has a small depth of field of view and sliding gate cannot meet the need of real time. In order to overcome these problems, multi-slice integration (MSI) method is proposed in this paper. First, the laser energy received by the PLRGI system is derived from radiative transfer theory. In addition, range intensity profiles (RIP) of MSI method is established. Experiments are carried out in lab to validate the RIP model and results show that theoretical data and experimental data are in good coincidence. Then the gate width and the number of pulse assigned to each slice are discussed. Finally, to prove the effectiveness of the MSI method, experiments are carried out in a boat tank. Results show that the MSI method is better than the fixed gate method for surveillance, and can acquire a clear image of the target at 18 m (4.5 times of the attenuation length) in the water with attenuation coefficient of 0.25 m−1.