The Potency of Lactobacillus fermentum and Moringa oleifera Extract with different Fermentation Time to improve the Nutrient content of Fermented Rice Bran

Abstract

The aim of the study was to evaluate Lactobacillus fermentum (L.fermentum) and Moringa oleifera (M.oleifera) extract for the future development synbiotic to improve the nutrient content of rice bran fermented products to support health. The treatments are divided into 7 treatments: 1:control (without probiotic and without M.oleifera extract); 2:(1% L. fermentum); 3:(1% L. fermentum + 1% M.oleifera extract); 4:(1% L. fermentum + 2% M.oleifera extract); 5:(2% L. fermentum; 6:(2% L. fermentum + 1% M.oleifera extract); 7:(2% L. fermentum + 2% M.oleifera extract), and then incubated at 37ºC, 24 hours. Isolate L. fermentum (108 cfu/ml) which has been incubated with M. oleifera extract, then sprayed on the rice bran according to the each treatment, then incubated an aerob condition for 5 and 10 days. After the fermentation period was complete, a proximate analysis was carried out. Results: The addition of L. fermentum and M.oleifera extract could increase the crude protein content from 12.66% to 15.94% (2% L.fermentum + 2% M.oleifera extract on 5 days of fermentation), decrease crude fiber from 21.28% to 13.50% (2% L.fermentum + 1% M.oleifera extract on 5 days of fermentation), increase nitrogen free extract (NFE) from 43.81% to 51.53% (2% L.fermentum + 1% M.oleifera extract on 5 days of fermentation), increase metabolizable energy (ME) from 3006.97 kcal/kg to 3294.96 kcal/kg (1% L.fermentum + 1% M.oleifera extract, on 5 days of fermentation). The addition of 1% and 2% L.fermentum as probiotic and 1% and 2% Moringa oleifera extract as prebiotic had potential properties as synbiotic.

Effect of probiotics and acidifiers on feed intake, egg mass, production performance, and egg yolk chemical composition in late-laying quails

“…Table-3 lists the average feed efficiency (%) with additional probiotics and acidifiers on late-laying quails. These results are in accordance with [ 12 ] research that probiotics ( L. acidophilus , L. casei , Lactococcus lactis , and Bifidobacterium spp.) give positive results on production performance (body weight gain, body weight, feed consumption, feed efficiency, and feed conversion ratio), carcass production (carcass weight and percentage), and mortality in Peking ducks.…”

“…with 1.2 × 10 9 concentration colony-forming units/mL showed positive results on growth performance (body weight, feed consumption, feed conversion ratio, carcass weight, and breast meat weight). Probiotics can increase broiler body weight and improve feed conversion ratio associated with increased feed efficiency [ 37 ], livestock growth performance, and feed efficiency [ 12 , 21 ].…”

“…Yulianto and Lokapirnasari [ 7 ] successfully isolated lactic acid probiotics from the digestive tract of native chickens. Probiotics improve egg quality and production [ 8 ], livestock performance and chicken meat quality [ 9 , 10 ], act as immunomodulators [ 11 ], and improve the quality of feedstuffs [ 12 ].…”

The potential of Moringa oleifera extract as a phytobiotic candidate for the growth of Lactobacillus fermentum bacteria by in vitro

“…In a study by Anudeep and Radha [9], Moringa oleifera leaf extracts contained oligosaccharides. Fructooligosaccharides and malto-oligosaccharides also have a significant effect of stimulating the growth of BAL organisms [12].…”

“…is one of the plants with high carbohydrate concentration. The total carbohydrate concentration in the ethanol extract is 42%, consisting of monosaccharides, disaccharides, and oligosaccharides, i.e., gut micro-flora nutrient [9]. Phytochemical compounds in Moringa oleifera are glycosides, triterpenes, flavonoids, luteolin, quercetin, tannins [10], also has proteins, fats, fibers, calcium, magnesium, phosphorus, potassium, copper, iron, vitamins, and essential amino acids [11].…”