The Potency of Sumba Ongole (So) Cattle: A Study of Genetic Characterization and Carcass Productivity

Abstract

ABSTRAKTelah dilakukan suatu penelitian untuk mengevaluasi keragaman genetik sapi Sumba Ongole (SO) berdasarkan DNA mikrosatelit dan mengkaji potensi sapi SO sebagai sapi potong lokal Indonesia berdasarkan kemampuan produksi karkasnya. Sebanyak 28 sampel darah sapi SO digunakan untuk melakukan karakterisasi genetik menggunakan 12 pasang primer DNA mikrosatelit yang direkomendasikan oleh FAO. Data produksi karkas dan bobot kulit berasal dari 506 ekor sapi SO yang dipotong di rumah potong hewan Karawaci, Banten, Indonesia. Nilai heterozigositas 12 lokus mikrosatelit berkisar antara 0,143 sampai dengan 1,000 (rata-rata 0,536). Nilai PIC tertinggi (0,814) ditemukan pada lokus TGLA122 sedangkan nilai terendah (0,280) ditemukan pada lokus BM1818. Sapi dengan bobot potong 351-475 kg merupakan sapi yang paling banyak dipotong pada tahun 2013 dan 2014 dengan persentase karkas antara 52,89% sampai dengan 53,43%. Persentase karkas tertinggi (56,34%) diperoleh dari sapi dengan bobot potong 626-650 kg. Sementara itu persentase karkas terendah (51,42%) diperoleh dari sapi dengan bobot potong 250-275 kg. Hasil karakterisasi genetik menunjukkan bahwa seluruh lokus mikrosatelit berada dalam kondisi beragam dan dapat digunakan untuk mendeteksi level keragaman genetik populasi sapi SO. Hasil studi produksi karkas menunjukkan bahwa sapi SO memiliki potensi yang sangat baik sebagai sapi potong bila dibandingkan dengan bangsa sapi potong lokal lain di Indonesia. ABSTRACTA study was conducted to assess the genetic characterization of the Sumba Ongole (SO) cattle based on DNA microsatellites and also to study the potency of SO cattle based on carcass productivity. Blood samples were collected from 28 individual cattle and 12 microsatellite primers as recommended by FAO were used to identify the genetic characterization of the SO cattle population. Data of carcass productivity were collected from 506 individual cattle that slaughtered in Karawaci abattoir, Banten, Indonesia. The heterozygosity values of microsatellite loci ranged from 0.143 to 1.000 (mean 0.536). The highest PIC values was 0.814 (locus TGLA122), while the lowest was 0.280 (locus BM1818). Cattle in range of 351-475 kg slaughter weight was most slaughtered in year 2013 and 2014 with carcass percentage ranged from 52.89% to 53.43%. The highest carcass percentage (56.34%) was obtained from cattle in range of 626-650 kg slaughter weight while the lowest (51.42%) was obtained from cattle in range of 250-275 kg slaughter weight. The results of genetic characterization showed that all microsatellite locus were highly polymorphic and highly informative for detecting the level of genetic diversity in the SO cattle population. The results of carcass productivity showed that the SO cattle has excellent potential as beef cattle compare with other local breeds cattle in Indonesia.

Association of growth hormone (GH) gene polymorphism with growth and carcass in Sumba Ongole (SO) cattle

“…M=DNA Ladder 100 bp; AA=Genotype AA; BB=Genotype BB; AB=Genotype AB. dressing percentage in the SO cattle obtained in this study was in agreement with Agung et al (2015) that reported the potency of the SO cattle as the beef cattle breed considering the high dressing percentage (>50%) for both the SO cattle in their natural habitat in the Sumba island, and also the SO cattle in the Java island.…”

“…The breeding program of the Ongole cattle was centralized in the Sumba island (East Nusa Tenggara Province), hereafter the Ongole cattle became known as Sumba Ongole cattle (Hardjosubroto, 2004). According to Agung et al (2015), the SO cattle has excellent potential as beef cattle based on the ability to gain higher dressing percentage (>50%) compare with other local breeds cattle in Indonesia. Recently, Indonesian farmers can do the selection in the SO cattle based on the MPPA value, breeding value and also performance test (Said et al, 2016a;Said et al, 2016b) but it is need more genetic information to determine the population for the breeding program.…”

Identification of a Differentiation Factor of Indonesian Ongole Cattle Breeds Based on Microsatellite Markers and Mitochondrial DNA

“…The blood samples were used in the DNA extraction process using the Genomic DNA Mini kit (Geneaid Biotech Ltd., Taiwan) following the manufacturer's protocol. A total of 12 microsatellite-labeled primers (part of the 30 primers recommended by Food and Agriculture Organization of the United Nations (FAO)) were used in the polymerase chain reaction (PCR) process (primers sequence, annealing temperature, the range of PCR product size, and label used were based on Agung et al (2015)). Amplification of the Cyt b gene was performed using primers based on Hartatik et al (2015) i.e.…”

“…Since then, the Ongole generations resulted from breeding programs in the Sumba Island have been known as Sumba Ongole (SO) cattle (Hardjosubroto, 2004). The SO cattle have excellent potential to gain higher dressing percentage (>50%) compared with other local cattle breeds in Indonesia (Agung et al, 2015).…”

Estimation of Most Probable Producing Ability Value for Calf Birth's Performance in Sumba Ongole Cows

“…The SO cattle is preferred by most raisers because of the quality of meat product (thickness and lean). Agung et al (2015) reported that the hot carcass weight of male SO cattle with slaughter weight 401 to 425 kg obtain 218.73±9.15 kg. To maintain and improve the excellence of the SO cattle, it is necessary to conduct the selection and breeding program based on their MPPA value.…”