The Potential Convention of Garlic and Black Seed Different Extracts as an Effective Treatment of Cryptosporidium Spp. : An Experimental Study

Sadek, Hesham A.; Abdel-Rahman, Salma M.; Bakir, Hanna Y.; Arafa, Mohsen I.; Ahmed, Ahmed A.; Gaber, Mona

doi:10.21608/jesp.2020.131094

Cited by 10 publications

(3 citation statements)

References 33 publications

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“…It has traditional nutritive and medicinal applications as an anti-infective, anti-parasitic, and anthelmintic agent [ 36 ]. Moreover, garlic demonstrated effective prophylactic and therapeutic effects against coccidian parasites [ 37 ]. Allicin induces changes in the intestinal microbiota of broiler chickens, has an antioxidant effect on Eimeria oocysts in broiler chickens [ 38 ], and efficiently inhibits the efficient sporulation of E. tenella [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Effect of Allicin and Alcoholic Garlic Extract on the Morphology and Infectivity of Eimeria tenella Oocysts in Chickens

Abd-ELrahman

Mohamed

et al. 2022

Animals

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Avian coccidiosis remains one of the major parasitic diseases that threaten the global poultry industry. Since prevention is superior to treatment, this study focuses on eliminating the infection outside the host. To determine their effect on the viability of Eimeria tenella oocysts in vitro, allicin and alcoholic garlic extract, which are natural, less toxic, and inexpensive products, were compared to KOH 5% (chemical disinfectant) using an in vitro culture system. Three concentrations of allicin (45, 90, and 180 mg/mL) and alcoholic garlic extract (90, 180, and 360 mg/mL, were used. Subsequently, destructive and sporulation-inhibiting effects on Eimeria oocysts were detected using light and electron microscopy. Young chickens were infected with treated sporulated oocysts to determine their effect on infectivity. After 7 days pi, the percentage of excreted oocysts (oocyst shedding) was determined, and the chickens were slaughtered for histopathological examination of the cecal tissues. Under an electron microscope, allicin at a concentration of 180 mg/mL and alcoholic garlic extract at a concentration of 360 mg/mL demonstrate a high oocysticidal activity with severe destruction of the oocyst wall and the appearance of pores. In addition, both concentrations directly affected the infectivity of sporulated oocysts by reducing the shedding of oocysts and the pathological lesions of infected young chickens. We concluded that the ability of Allicin and alcoholic garlic extract to eliminate Eimeria oocysts makes them superior to chemical disinfectants as a disinfectant.

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Section: Discussionmentioning

confidence: 99%

Comparative Effect of Allicin and Alcoholic Garlic Extract on the Morphology and Infectivity of Eimeria tenella Oocysts in Chickens

Abd-ELrahman

Mohamed

et al. 2022

Animals

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“…Group 2 was left untreated and functioned as the positive control group. Ten days post infection (PI), group3 (AS‐treated group) was orally given A. sativum at a dose of 50 mg/kg (Sadek et al, 2020), whereas group 4 and group 5 were orally given ZnO‐NPs at a dose of 50 mg/kg (Saadatmand et al, 2021), and ZnO‐NPs at a dose of 100 mg/kg (Saadatmand et al, 2021), respectively, for 7 consecutive days (Gaafar, 2007). On day 21 PI, mice were euthanized, fecal pellets were collected, and the ilea, livers, and lungs were removed for the further studies.…”

Section: Methodsmentioning

confidence: 99%

Green synthesis of zinc oxide/Allium sativum nano‐composite and its efficacy against murine cryptosporidiosis

Hamdy,

Ismail,

El‐Askary

et al. 2024

Microscopy Res & Technique

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Cryptosporidiosis is a global health problem threats life of immunocompromised patients. Allium sativum (A. sativum) is one of the therapeutic options for cryptosporidiosis. This study develops green synthesized ZnO‐NPs based on A. sativum extract, and assesses its therapeutic application in treating experimental cryptosporidiosis in immunosuppressed mice. FTIR, scanning electron microscopy, and zeta analyzer were used for characterization of bio ZnO‐NPs. The morphology of prepared materials appeared as sponge with many pores on the whole surface that allows the feasibility of bio ZnO‐NPs for different biological activities. Its structural analysis was highly stabilized with negative charge surface which indicated for well distribution into the parasite matrix. Twenty‐five immunosuppressed Cryptosporidium parvum infected mice, classified into 5 groups were sacrificed at 21th day after infection with evaluation of parasitological, histopathological, oxidative, and proinflammatory biomarkers. Treated mice groups with 50 and 100 mg/kg of AS/ZnO‐NPs showed a highly significant decline (79.9% and 83.23%, respectively) in the total number of expelled oocysts. Both doses revealed actual amelioration of the intestinal, hepatic, and pulmonary histopathological lesions. They also significantly produced an increase in GSH values and improved the changes in NO and MDA levels, and showed high anti‐inflammatory properties. This study is the first to report green synthesis of ZnO/A. sativum nano‐composite as an effective therapy in treating cryptosporidiosis which gave better results than using A. sativum alone. It provides an economical and environment‐friendly approach towards novel delivery synthesis for antiparasitic applications.Research Highlights Green synthesis of ZnO‐NPs was developed using A. sativum extract. The morphology of prepared ZnO‐NPs appeared as sponge with many pores on SEM The study evaluates its therapeutic efficacy against murine cryptosporidiosis The green synthesized ZnO‐NPs significantly reduced percent of oocyst shedding, improved the pathological changes, and showed high antioxidant and anti‐inflammatory potentials.

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“…Moreover, garlic crude extract showed great activity against worms and protozoon parasites Cryptosporidium spp. in different animal models [109][110][111].…”

Section: Antiparasiticmentioning

confidence: 99%

Potential Effects of Garlic (Allium sativum L.) on the Performance, Immunity, Gut Health, Anti-Oxidant Status, Blood Parameters, and Intestinal Microbiota of Poultry: An Updated Comprehensive Review

Abd El-Ghany

2024

Animals

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The use of antibiotics as growth promoters or for the prevention of some poultry diseases has faced global concern and serious criticism. Their addition to poultry feed has shown hazardous effects, including the development of antimicrobial resistance and a potentially harmful effect on human health. To eliminate these threats, there is increasing interest in natural alternatives. Plant derivatives such as garlic (Allium sativum L.) and its derivatives are presently extensively used in the poultry production system. The dietary supplementation of broilers and layers with garlic induced improvement in the production parameters, carcass quality, and intestinal integrity. The modulation of the immune response against some important viral diseases has resulted from the supplementation of poultry with garlic. Moreover, garlic has been shown to modulate gut health through antibacterial and antiparasitic activities. Treatment with garlic can also mitigate oxidative stress and reduce free-radical production. The reduction of cholesterol levels and improvement of some liver and blood parameters were also reported following the dietary inoculation of garlic. This review was designed to investigate the influence of garlic as a dietary additive on the performance, immunity, gut health, anti-oxidant status, blood parameters, and intestinal microbiota of poultry.

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The Potential Convention of Garlic and Black Seed Different Extracts as an Effective Treatment of Cryptosporidium Spp. : An Experimental Study

Cited by 10 publications

References 33 publications

Comparative Effect of Allicin and Alcoholic Garlic Extract on the Morphology and Infectivity of Eimeria tenella Oocysts in Chickens

Comparative Effect of Allicin and Alcoholic Garlic Extract on the Morphology and Infectivity of Eimeria tenella Oocysts in Chickens

Green synthesis of zinc oxide/Allium sativum nano‐composite and its efficacy against murine cryptosporidiosis

Potential Effects of Garlic (Allium sativum L.) on the Performance, Immunity, Gut Health, Anti-Oxidant Status, Blood Parameters, and Intestinal Microbiota of Poultry: An Updated Comprehensive Review

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