The potential for bioaugmentation of sand filter materials from waterworks using bacterial cultures degrading 4-chloro-2-methylphenoxyacetic acid

Krüger, Urse Scheel; Johnsen, Anders R.; Burmølle, Mette; Aamand, Jens; Sørensen, Sebastian R.

doi:10.1002/ps.3796

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…The strain Sphingomonas sp. PM2, which is capable of degrading several phenoxy acid herbicides, was isolated in the laboratory and characterised by Johannesen ( 2002 ); it is a promising candidate for remediation of a broad range of herbicide concentrations (Qiu et al 2014 ; Krüger et al 2015 ). Both strains were stored in 40 % sterile glycerol at −80 °C and thawed and pre-cultivated before the experiments.…”

Section: Methodsmentioning

confidence: 99%

Bioaugmentation potential of free and formulated 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in soil, sand and water

2016

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Pesticides are used extensively worldwide, which has led to the unwanted contamination of soil and water resources. Former use of the herbicide 2,6-dichlorobenzonitrile (dichlobenil) has caused pollution of ground and surface water resources by the stable degradation product 2,6-dichlorobenzamide (BAM) in several parts of Europe, which has resulted in the costly closure of several drinking water wells. One strategy for preventing this in future is bioaugmentation using bacterial degraders. BAM-degrading Aminobacter sp. MSH1 was therefore formulated into dried beads and tests undertaken to establish their potential for use in the remediation of polluted soil, sand and water. The formulation procedure included freeze drying, combined with trehalose addition for cell wall protection, thus ensuring a high amount of viable cells following prolonged storage at room temperature. The beads were round-shaped pellets with a diameter of about 1.25 mm, a dry matter content of approximately 95 % and an average viable cell content of 4.4 × 109 cells/g bead. Formulated MSH1 cells led to a similar, and frequently even faster, BAM mineralisation (20–65 % 14CO2 produced from 14C-labelled BAM) in batch tests conducted with sand, water and different soil moisture contents compared to adding free cells. Furthermore, the beads were easy to handle and had a shelf life of several months.

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Section: Methodsmentioning

confidence: 99%

Bioaugmentation potential of free and formulated 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in soil, sand and water

2016

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“…strain ERG5 was previously isolated from an enriched bacterial community that originated from an aquifer located below a conventionally treated agricultural field at Fladerne Creek in Denmark ( 2 ). This strain can readily mineralize MCPA at both low and high concentrations (10 µg·L −1 to 10 mg·L −1 ), making it a strong candidate for bioremediation purposes ( 3 ). Sphingomonas sp.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of MCPA-Degrading Sphingomonas sp. Strain ERG5, Isolated from a Groundwater Aquifer in Denmark

et al. 2015

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Sphingomonas sp. strain ERG5 was isolated from a bacterial community, originating from a groundwater aquifer polluted with low pesticide concentrations. This bacterium degrades 2-methyl-4-chlorophenoxyacetic acid (MCPA) in a wide spectrum of concentrations and has been shown to function in bioaugmented sand filters. Genes associated with MCPA degradation are situated on a putative conjugative plasmid.

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“…When indigenous microbes lack the capacity to degrade PAHs, the introduction of PAH degraders isolated from other PAH-contaminated sites, 6,7 known as bioaugmentation (BA), is a promising technology to encourage PAH degradation. 8,9 However, the efficacy of BA is variable, since the survival and the PAH-degrading ability of the introduced microorganisms are highly dependent on environmental conditions. 10,11 To overcome these difficulties, autochthonous bioaugmentation (ABA) has been proposed as an alternative method.…”

Section: Introductionmentioning

confidence: 99%

“…PAHs in a polluted environment can be partially degraded by the autochthonous microbial population via natural attenuation. When indigenous microbes lack the capacity to degrade PAHs, the introduction of PAH degraders isolated from other PAH-contaminated sites, , known as bioaugmentation (BA), is a promising technology to encourage PAH degradation. , However, the efficacy of BA is variable, since the survival and the PAH-degrading ability of the introduced microorganisms are highly dependent on environmental conditions. , To overcome these difficulties, autochthonous bioaugmentation (ABA) has been proposed as an alternative method . ABA is defined as a BA technology that uses indigenous degraders at the polluted sites (soil, sediment, and water) with the aim of accelerating and enhancing the biodegradation potential …”

Section: Introductionmentioning

confidence: 99%

Autochthonous Bioaugmentation-Modified Bacterial Diversity of Phenanthrene Degraders in PAH-Contaminated Wastewater as Revealed by DNA-Stable Isotope Probing

Luo

Zhang

et al. 2018

Environ. Sci. Technol.

107

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To reveal the mechanisms of autochthonous bioaugmentation (ABA) in wastewater contaminated with polycyclic aromatic hydrocarbons (PAHs), DNA-stable-isotope-probing (SIP) was used in the present study with the addition of an autochthonous microorganism Acinetobacter tandoii LJ-5. We found LJ-5 inoculum produced a significant increase in phenanthrene (PHE) mineralization, but LJ-5 surprisingly did not participate in indigenous PHE degradation from the SIP results. The improvement of PHE biodegradation was not explained by the engagement of LJ-5 but attributed to the remarkably altered diversity of PHE degraders. Of the major PHE degraders present in ambient wastewater ( Rhodoplanes sp., Mycobacterium sp., Xanthomonadaceae sp. and Enterobacteriaceae sp.), only Mycobacterium sp. and Enterobacteriaceae sp. remained functional in the presence of strain LJ-5, but five new taxa Bacillus, Paenibacillus, Ammoniphilus, Sporosarcina, and Hyphomicrobium were favored. Rhodoplanes, Ammoniphilus, Sporosarcina, and Hyphomicrobium were directly linked to, for the first time, indigenous PHE biodegradation. Sequences of functional PAH-RHD genes from heavy fractions further proved the change in PHE degraders by identifying distinct PAH-ring hydroxylating dioxygenases between ambient degradation and ABA. Our findings indicate a new mechanism of ABA, provide new insights into the diversity of PHE-degrading communities, and suggest ABA as a promising in situ bioremediation strategy for PAH-contaminated wastewater.

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The potential for bioaugmentation of sand filter materials from waterworks using bacterial cultures degrading 4-chloro-2-methylphenoxyacetic acid

Abstract: This study shows that an increase in the potential for mineralisation of low herbicide concentrations in sand filter materials can be achieved by inoculating with bacterial degrader cultures. © 2014 Society of Chemical Industry.

Cited by 7 publications

References 31 publications

Bioaugmentation potential of free and formulated 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in soil, sand and water

Bioaugmentation potential of free and formulated 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in soil, sand and water

Draft Genome Sequence of MCPA-Degrading Sphingomonas sp. Strain ERG5, Isolated from a Groundwater Aquifer in Denmark

Autochthonous Bioaugmentation-Modified Bacterial Diversity of Phenanthrene Degraders in PAH-Contaminated Wastewater as Revealed by DNA-Stable Isotope Probing

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