Spectroscopic techniques, such as Fourier-transform infrared (FTIR) spectroscopy, are used to study the interaction of light with biological materials. This interaction forms the basis of many analytical assays used in disease screening and diagnosis, microbiological studies, forensic and environmental investigations. Advantages of spectrochemical analysis are its low cost, minimal sample preparation, non-destructive nature and substantially accurate results. However, there is now an urgent need for repetition and validation of these methods in large-scale studies and across different research groups, which would bring the method closer to clinical and/or industrial implementation. In order for this to succeed, it is important to understand and reduce the effect of random spectral alterations caused by inter-individual, inter-instrument and/or inter-laboratory variations, such as variations in air humidity and CO2 levels, and the aging of instrumental parts.Thus, it is evident that spectral standardization is crucial for the widespread adoption of these spectrochemical technologies. By using calibration transfer procedures, where the spectral response of a secondary instrument is standardized to resemble the spectral response of a primary instrument, different sources of variations can be normalized into a single model using computational-based methods, such as direct standardization (DS) and piecewise direct standardization (PDS); therefore, measurements performed under different conditions can generate the same result, eliminating the need for a full recalibration. In this paper, we have constructed a protocol for model standardization using different transfer technologies described for FTIR spectrochemical applications. This is a critical step towards the construction of a practical spectrochemical analysis model for daily routine analysis, where uncertain and random variations are present. 4 worldwide are developing spectrochemical approaches for diagnosis, discrimination and monitoring of diseases, as well as for other uses. Combination of multiple datasets would facilitate the conduction of large-scale studies which are still lacking in the field of bio-spectroscopy.
Sensor-based technologiesSensor-based technologies are an integral part of daily life ranging from locating sensorbased technology, such as global positioning system (GPS) 6 , to image biosensors, such as X-rays 7-10 and γ-rays [11][12][13] , which are used extensively for medical applications. Other powerful approaches that make use of sensor-based technologies toward medical disease examination and diagnostics include circular dichroism (CD) spectroscopy 14-17 , ultraviolet (UV) or visible spectroscopy 18,19 , fluorescence 20-24 , nuclear magnetic resonance (NMR) spectroscopy 25-29 and ultrasound (US) 7,30-
.Over the last two decades, optical biosensors employing vibrational spectroscopy, particularly IR spectroscopy, have seen tremendous progress in biomedical and biological research. A number of studies using the above-mentioned methods ha...