The potential function of KIF17 in large yellow croaker (Larimichthys crocea) spermatid remodeling: molecular characterization and expression pattern during spermiogenesis

Wang, Jingqian; Liu, Zhao; Gao, Xinming; Du, Chang; Hou, Cong-Cong; Tang, Daojun; Liu, Bao; Shen, Wei; Zhu, Junquan

doi:10.1007/s10695-021-01035-3

Cited by 3 publications

(7 citation statements)

References 36 publications

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“…This particular interaction reflects the uniqueness of the KIF17b mediated transport of ACT [ 27 ]. In the present study, the predicted Pe -KIF17 protein had the same structural features as previously reported KIF17 [ 17 , 18 ]. Multiple sequence comparisons with KIF17 amino acid sequences from other species showed that their motor domains were highly conserved and contained microtubule-binding sites and ATP hydrolysis sites.…”

Section: Discussionsupporting

confidence: 85%

“…This coincides with the high expression of kif17 mRNA in stage IV of L. polyactis and L. crocea spermiogenesis. The testes of both contain numerous spermatids [ 17 , 18 , 34 ], which suggests the involvement in spermiogenesis, due to the high expression of KIF17 in the testes of L. crocea and L. polyactis [ 17 , 18 ]. Corresponding findings regarding the expression of kif17 mRNA revealed the highest expression in the CF, where spermatids underwent morphological transformation into sperm throughout spermiogenesis, in contrast to other tissues, during July of the breeding season.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have found that KIF17b and Spatial also exist in the manchette region and are continuously expressed in the sperm tails [ 19 ] and that KIF17b is expressed throughout spermatogenesis persists in the sperm tail [ 20 , 38 ]. Furthermore, in L. polyactis and L. crocea , KIF17 is involved in spermatid remodeling, including spermatid nucleus reshaping and sperm tail formation, through perinuclear microtubules during spermiogenesis [ 17 , 18 ]. Based on these findings, a model involving Pe -KIF17 in spermatid remodeling can be proposed ( Figure 9 A1,A2).…”

Section: Discussionmentioning

confidence: 99%

“…KIF17 is involved in spermiogenesis via two mechanisms. In the first mechanism, KIF17 operates in a microtubule-dependent manner, similar to other kinesins [ 17 , 18 ]. In mice, KIF17 (designated KIF17b in the testes) co-localized with Spatial in the manchette structure, where the sperm nucleus is slightly elongated in shape than a sphere.…”

Section: Introductionmentioning

confidence: 99%

“…As chromatin thickens, the manchette structure moves to one side of the nucleus and continues to be located in the principal piece of the sperm tail, suggesting that KIF17b may be involved in spermatid nucleus reshaping via the manchette [ 19 ]. In Larimichthys polyactis and Larimichthys crocea , KIF17 is involved in spermatid remodeling, including spermatid nucleus reshaping and tail formation through perinuclear microtubules during spermiogenesis [ 17 , 18 ]. Studies on vertebrates have shown that KIF17 and the manchette are involved in spermiogenesis; however, related studies in mollusks are yet to be conducted.…”

Section: Introductionmentioning

confidence: 99%

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Expression Dynamics Indicate Potential Roles of KIF17 for Nuclear Reshaping and Tail Formation during Spermiogenesis in Phascolosoma esculenta

Pan,

Wang,

Gao

et al. 2023

IJMS

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Kinesin family member17 (KIF17), a homologous dimer of the kinesin-2 protein family, has important microtubule-dependent and -independent roles in spermiogenesis. Little is known about KIF17 in the mollusk, Phascolosoma esculenta, a newly developed mariculture species in China. Here, we cloned the open reading frame of Pe-kif17 and its related gene, Pe-act, and performed bioinformatics analysis on both. Pe-KIF17 and Pe-ACT are structurally conserved, indicating that they may be functionally conserved. The expression pattern of kif17/act mRNA performed during spermiogenesis revealed their expression in diverse tissues, with the highest expression level in the coelomic fluid of P. esculenta. The expressions of Pe-kif17 and Pe-act mRNA were relatively high during the breeding season (July–September), suggesting that Pe-KIF17/ACT may be involved in spermatogenesis, particularly during spermiogenesis. Further analysis of Pe-kif17 mRNA via fluorescence in situ hybridization revealed the continuous expression of this mRNA during spermiogenesis, suggesting potential functions in this process. Immunofluorescence showed that Pe-KIF17 co-localized with α-tubulin and migrated from the perinuclear cytoplasm to one side of the spermatid, forming the sperm tail. Pe-KIF17 and Pe-ACT also colocalized. KIF17 may participate in spermiogenesis of P. esculenta, particularly in nuclear reshaping and tail formation by interacting with microtubule structures similar to the manchette. Moreover, Pe-KIF17 with Pe-ACT is also involved in nuclear reshaping and tail formation in the absence of microtubules. This study provides evidence for the role of KIF17 during spermiogenesis and provides theoretical data for studies of the reproductive biology of P. esculenta. These findings are important for spermatogenesis in mollusks.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Expression Dynamics Indicate Potential Roles of KIF17 for Nuclear Reshaping and Tail Formation during Spermiogenesis in Phascolosoma esculenta

Pan,

Wang,

Gao

et al. 2023

IJMS

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Identifying Candidate Genes for Litter Size and Three Morphological Traits in Youzhou Dark Goats Based on Genome-Wide SNP Markers

Sun,

Niu,

Jiang

et al. 2023

Genes

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This study aimed to reveal the potential genetic basis for litter size, coat colour, black middorsal stripe and skin colour by combining genome-wide association analysis (GWAS) and selection signature analysis and ROH detection within the Youzhou dark (YZD) goat population (n = 206) using the Illumina GoatSNP54 BeadChip. In the GWAS, we identified one SNP (snp54094-scaffold824-899720) on chromosome 11 for litter size, two SNPs on chromosome 26 (snp11508-scaffold142-1990450, SORCS3) and chromosome 12 (snp55048-scaffold842-324525, LOC102187779) for coat colour and one SNP on chromosome 18 (snp56013-scaffold873-22716, TCF25) for the black middorsal stripe. In contrast, no SNPs were identified for skin colour. In selection signature analysis, 295 significant iHS genomic regions with a mean |iHS| score > 2.66, containing selection signatures encompassing 232 candidate genes were detected. In particular, 43 GO terms and one KEGG pathway were significantly enriched in the selected genes, which may contribute to the excellent environmental adaptability and characteristic trait formation during the domestication of YZD goats. In ROH detection, we identified 4446 ROH segments and 282 consensus ROH regions, among which nine common genes overlapped with those detected using the iHS method. Some known candidate genes for economic traits such as reproduction (TSHR, ANGPT4, CENPF, PIBF1, DACH1, DIS3, CHST1, COL4A1, PRKD1 and DNMT3B) and development and growth (TNPO2, IFT80, UCP2, UCP3, GHRHR, SIM1, CCM2L, CTNNA3 and CTNNA1) were revealed by iHS and ROH detection. Overall, this study is limited by the small population size, which affects the results of GWAS to a certain extent. Nevertheless, our findings could provide the first overview of the genetic mechanism underlying these important traits and provide novel insights into the future conservation and utilisation of Chinese goat germplasm resources.

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The Effect of Prohibitins on Mitochondrial Function during Octopus tankahkeei Spermiogenesis

Wang

Gao

et al. 2023

IJMS

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Mitochondria are essential for spermiogenesis. Prohibitins (PHBs; prohibitin 1, PHB1 or PHB, and prohibitin 2, PHB2) are evolutionarily conserved and ubiquitously expressed mitochondrial proteins that act as scaffolds in the inner mitochondrial membrane. In this study, we analyzed the molecular structure and dynamic expression characteristics of Ot-PHBs, observed the colocalization of Ot-PHB1 with mitochondria and polyubiquitin, and studied the effect of phb1 knockdown on mitochondrial DNA (mtDNA) content, reactive oxygen species (ROS) levels, and apoptosis-related gene expression in spermatids. Our aim was to explore the effect of Ot-PHBs on mitochondrial function during the spermiogenesis of Octopus tankahkeei (O. tankahkeei), an economically important species in China. The predicted Ot-PHB1/PHB2 proteins contained an N-terminal transmembrane, a stomatin/prohibitin/flotillin/HflK/C (SPFH) domain (also known as the prohibitin domain), and a C-terminal coiled-coil domain. Ot-phb1/phb2 mRNA were widely expressed in the different tissues, with elevated expression in the testis. Further, Ot-PHB1 and Ot-PHB2 were highly colocalized, suggesting that they may function primarily as an Ot-PHB compiex in O. tankahkeei. Ot-PHB1 proteins were mainly expressed and localized in mitochondria during spermiogenesis, implying that their function may be localized to the mitochondria. In addition, Ot-PHB1 was colocalized with polyubiquitin during spermiogenesis, suggesting that it may be a polyubiquitin substrate that regulates mitochondrial ubiquitination during spermiogenesis to ensure mitochondrial quality. To further investigate the effect of Ot-PHBs on mitochondrial function, we knocked down Ot-phb1 and observed a decrease in mtDNA content, along with increases in ROS levels and the expressions of mitochondria-induced apoptosis-related genes bax, bcl2, and caspase-3 mRNA. These findings indicate that PHBs might influence mitochondrial function by maintaining mtDNA content and stabilizing ROS levels; in addition, PHBs might affect spermatocyte survival by regulating mitochondria-induced apoptosis during spermiogenesis in O. tankahkeei.

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The potential function of KIF17 in large yellow croaker (Larimichthys crocea) spermatid remodeling: molecular characterization and expression pattern during spermiogenesis

Cited by 3 publications

References 36 publications

Expression Dynamics Indicate Potential Roles of KIF17 for Nuclear Reshaping and Tail Formation during Spermiogenesis in Phascolosoma esculenta

Expression Dynamics Indicate Potential Roles of KIF17 for Nuclear Reshaping and Tail Formation during Spermiogenesis in Phascolosoma esculenta

Identifying Candidate Genes for Litter Size and Three Morphological Traits in Youzhou Dark Goats Based on Genome-Wide SNP Markers

The Effect of Prohibitins on Mitochondrial Function during Octopus tankahkeei Spermiogenesis

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