The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia

Nisa, Indah Khoirun; Prabaningtyas, Sitoresmi; Lukiati, Betty; SAPTAWATI, RINA TRITURANI; Rodiansyah, Achmad

doi:10.13057/biodiv/d220106

Cited by 6 publications

(4 citation statements)

References 22 publications

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“…AL3.11 isolate has a 99.53% similarity with Bacillus paramycoides strain MCCC 1A04098. According to Nisa et al (2021), Bacillus paramycoides bacteria found in Ngebel Lake was also known to produce amylase. Microorganisms can produce amylase enzymes, especially from the Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Biodiversity of hydrolytic enzymes-producing soil bacteria from a Durian Park, Jombang, Indonesia: Beneficial prospect for sustainable agriculture

NI’MATUZAHROH,

AFFANDI,

SUPRIYANTO

et al. 2024

Biodiversitas

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Abstract. Ni’matuzahroh, Affandi M, Supriyanto A, Rustantina B, Jaiyah LA, Rahmawati A, Nurhayati H, Sari SK, Khiftiyah AM, Huri D. 2024. Biodiversity of hydrolytic enzymes-producing soil bacteria from a Durian Park, Jombang, Indonesia: Beneficial prospect for sustainable agriculture. Biodiversitas 25: 392-403. Biofertilizer is an alternative to chemical fertilizer that can enhance plant growth but is specific to certain plants and soils. Various biofertilizers have been produced, but the specific biofertilizers to enhance durian growth have not been widely available. This study aims to determine the potential of hydrolytic enzyme-producing bacteria, one of the microbial components of biofertilizers, in the De Durian Park (DDP) area, Wonosalam, Jombang District, Indonesia. The bacteria were isolated using the selective medium plating technique. The obtained isolates were screened for their ability to produce cellulase, amylase, protease, and lipase. The clear zone around the colony indicated the ability of the isolates to produce hydrolytic enzymes, and then the clear zone index was obtained. The best three isolates for each enzyme production were characterized macroscopically, microscopically, and biochemically, then identified using 16S rRNA gene sequence analysis. Fifty-two isolates were successfully obtained from the soil of DDP. The best three cellulolytic bacteria had indices ranging from 1.83 to 2.14, categorized as moderate-strong, identified as Bacillus anthracis, B. pacificus, and B. cereus. The amylolytic bacteria ranged from 1.25 to 1.35, categorized as moderate, and identified as Bacillus anthracis, B. paranthracis, and B. paramycoides. The proteolytic bacteria ranged from 1.33 to 1.50, categorized as moderate, identified as B. pumilus, B. paramycoides, and B. cereus. The lipolytic bacteria ranged from 1.94 to 3.87, categorized as moderate-strong, and were identified as Enterobacter sichuanensis, B. pacificus, and E. asburiae. These cellulolytic, amylolytic, proteolytic, and lipolytic bacteria have the potency as candidates for a biofertilizer formula.

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Section: Discussionmentioning

confidence: 99%

Biodiversity of hydrolytic enzymes-producing soil bacteria from a Durian Park, Jombang, Indonesia: Beneficial prospect for sustainable agriculture

NI’MATUZAHROH,

AFFANDI,

SUPRIYANTO

et al. 2024

Biodiversitas

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“…The amylase activity value at day 0 is quite high, this might be due to the measurement of the enzyme activity using the DNS method, in which reduc- ing sugar formed from a carbon source (starch) is used by bacteria for the initial stages of growth; then, the bacteria will use the carbon source for the production of enzymes. The amylase activity of Bacillus cereus KN isolated from Ranu Ngebel and incubated for three days was 0.016 U/mL, while strain G20 isolated from Ranu Grati was lower at about 0.0001 U/mL (Nisa et al 2021). Luang et al ( 2019) found Bacillus sp.…”

Section: Crude Enzyme Production Of a Selected Isolate And Measuremen...mentioning

confidence: 99%

Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase

Agustiani,

Oedjijono,

Rahmani

et al. 2023

J.Tropical Biodiversity Biotechnology

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The objectives of the study were to isolate amylolytic bacteria from the rhizosphere and plant tissue of Canna edulis Ker., as well as litter; to know oligosaccharide compounds produced from starch hydrolyzed by the bacterial enzymes, and to identify the amylolytic bacteria based on phenetic and 16S rRNA gene sequences. From the rhizosphere, Canna plant tissue, and litters obtained thirty-two amylolytic bacterial isolates. Eight isolates (TH6, TH7, T5, T10, D2, D3, A3, S1) produced high clear zone diameters ranging from 18-30 mm; especially an isolate T10, which was consistent in producing a total clear zone diameter of 20 mm. The hydrolysate of starch hydrolysed by the T10 amylase resulted in three oligosaccharide compounds maltotriose, maltotetraose, and maltopentose. The amylase activity of isolate T10 was optimal at a temperature of 40°C and pH at 0.801 U/mL. The isolate T10 was identified as a species member of Bacillus toyonensis based on phenotyphic characterization and 16S rDNA gene sequencing analysis with a similarity value of 99.93%

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“…The measurement of reducing groups produced by the different enzymes was performed using the 3,5dinitro salicylic acid (DNS) technique (Nisa et al, 2021). The choice of standard for enzyme analysis depended on the specific kind of enzyme being studied.…”

Section: Quantifying Enzymatic Activitymentioning

confidence: 99%

Sustainable and Eco-Friendly Production of Xylanase and Other Enzymes from Tomato Paste through Solid-State Fermentation Using Bacillus safensis MABS6

Hassan Alshawi

2023

Catrina: The International Journal of Environmental Sciences

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This study aimed to establish a sustainable and eco-friendly approach for the production of enzymes, including xylanase, exo-polygalacturonase (exo-PG), cellulase (CMCase), and αamylase, using tomato pomace through solid-state fermentation (SSF) with Bacillus safensis. Kinetic studies revealed that xylanase and exo-PG exhibited their peak activities early in the fermentation process, reaching approximately 110 IU/mL and 70 IU/mL, respectively. In contrast, CMCase and α-amylase activities remained relatively constant, maintaining average levels of 19.8 IU/mL and 22.4 IU/mL throughout the fermentation process. Further investigations in a plate-type bioreactor unveiled the significant impact of aeration on enzyme activities. Aeration positively enhanced the activities of xylanase and CMCase, while it had an inverse effect on exo-PG and αamylase activities. The focus of our study was to establish the optimal conditions for xylanase production, given its versatility and industrial value. The highest productivity was achieved at a pH of 5 and a temperature of 50 °C. Additionally, the presence of Mg+2 ions positively influenced enzymatic activity, whereas the presence of Hg +2 and Cu +2 ions acted as strong inhibitors. Furthermore, our results demonstrated the remarkable resilience and stability of xylanase across a wide pH range (pH 3-12) and temperatures (30 °C to 60 °C). Overall, these findings contribute to the development of greener enzyme production strategies and provide valuable insights into their widespread applicability across various industrial sectors.

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The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia

Cited by 6 publications

References 22 publications

Biodiversity of hydrolytic enzymes-producing soil bacteria from a Durian Park, Jombang, Indonesia: Beneficial prospect for sustainable agriculture

Biodiversity of hydrolytic enzymes-producing soil bacteria from a Durian Park, Jombang, Indonesia: Beneficial prospect for sustainable agriculture

Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase

Sustainable and Eco-Friendly Production of Xylanase and Other Enzymes from Tomato Paste through Solid-State Fermentation Using Bacillus safensis MABS6

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