The potential of archaeosomes as carriers of pDNA into mammalian cells

Abstract

This paper describes the formulation of archaeosomes and the evaluation of their abilities to facilitate in vitro DNA delivery. Lipids of the H.hispanica 2TK2 strain were used in archaeosome formation, which is formulated by mixing H.hispanica 2TK2 lipids with plasmid DNA encoding green fluorescent protein (GFP) or β-galactosidase (β-gal). Archaeosome/pDNA formation and unbound DNA were monitored by agarose gel electrophoresis. The archaeosome formulations were visualized by AFM and TEM. The zeta potential analysis showed the archaeosomes to be electronegative. The composition of archaeosomes and the DNA dose for transient transfection into HEK293 cells were optimized, and the relationship between the structure and activity of archaeosomes in DNA delivery was investigated. By themselves, archaeosomes showed low efficiency for DNA delivery, due to their anionic nature. By formulating archaeosomes with a helper molecule, such as DOTAP, CaCl2, or LiCl, the capability of archaeosomes for gene transfection is significantly enhanced. The transfection profiles of efficient archaeosomes are proved to have a long shelf-life when maintained at room temperature. Thus, the archaeal lipids have the potential to be used as transfection reagents in vitro.

Combination therapy using human papillomavirus L1/E6/E7 genes and archaeosome: a nanovaccine confer immuneadjuvanting effects to fight cervical cancer

“…For the latter, manufacturing of the surface localization of plasmid on lipid vesicles, plasmid DNA was surface-bound by incubating empty archaeosomes at 37 °C with shaking overnight. To improve complex formation between pDNA and archaeosomes, CaCl 2 as a helper molecule was also added into the medium 20 . In the end, the suspensions were sonicated…”

“…They are more resistant to chemical hydrolysis, oxidation, bile salts, acidic or alkaline pH, and high temperatures with various vaccine and drug delivery applications 17 . Therefore, liposomes formed by archaebacterial membranes lipids represent a promising alternative to the classical liposomes and virosoms, consequently found extensive applications in vaccine and drug delivery [18][19][20] .…”

Preparation, characterization, and in vitro evaluation of isoniazid and rifampicin‐loaded archaeosomes

“…Previous studies showed that the particles with zeta potentials <−30 mV are normally considered stable, and relatively high percentage of drug entrapment was recorded when compared to positively charged liposomes . In addition to this, if all the particles in a suspension have a large negative potential then they will tend to repel each other and have tendency to aggregate …”

Preparing Nanoarchaeosome Containing Triptorelin Acetate and Evaluation of Its Cellular Toxic Effect on PC3 Prostate Cancer Cell Line

“…In order to prepare archaeosome containing triptorelin acetate, we added hydrated medium containing mg of triptorelin acetate into the thin layer of lipid film, which was a homogeneous mixture of 5.42 mg cholesterol and 12.6 polar lipids of archaebacterial. Following that, sonication was performed for 10 minutes with a frequency of 60 Hz (Bandelin Sonorex Digitec) in order to harmonize the archaeosomes and preparation of a formulation with a better quality (17).…”