The potential of backscattering interferometry as an in vitro clinical diagnostic tool for the serological diagnosis of infectious disease

Kussrow, Amanda; Enders, Carolyn S.; Castro, Arnold R.; Cox, David L.; Ballard, Ronald C.; Bornhop, Darryl J.

doi:10.1039/c0an00098a

Cited by 18 publications

(20 citation statements)

References 14 publications

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“…BI can detect both free solution or surface immobilized molecular interactions with unprecedented limits in microfluidic devices (picoliter detection volume) and allows real-time determination of binding constants spanning from micro-to picomole. Kussrow et al have shown the potential of utilizing BI for rapid detection of purified total human IgG from seropositive syphilis patients using a purified recombinant treponenmal antigen r17, demonstrating the prospect of using this approach for serological diagnosis in clinical samples [26].…”

Section: Optical Transducermentioning

confidence: 99%

Advances and challenges in biosensor-based diagnosis of infectious diseases

Sin

Mach

Wong

et al. 2014

Expert Review of Molecular Diagnostics

333

238

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Rapid diagnosis of infectious diseases and timely initiation of appropriate treatment are critical determinants that promote optimal clinical outcomes and general public health. Conventional in vitro diagnostics for infectious diseases are time-consuming and require centralized laboratories, experienced personnel and bulky equipment. Recent advances in biosensor technologies have potential to deliver point-of-care diagnostics that match or surpass conventional standards in regards to time, accuracy and cost. Broadly classified as either label-free or labeled, modern biosensors exploit micro- and nanofabrication technologies and diverse sensing strategies including optical, electrical and mechanical transducers. Despite clinical need, translation of biosensors from research laboratories to clinical applications has remained limited to a few notable examples, such as the glucose sensor. Challenges to be overcome include sample preparation, matrix effects and system integration. We review the advances of biosensors for infectious disease diagnostics and discuss the critical challenges that need to be overcome in order to implement integrated diagnostic biosensors in real world settings.

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Section: Optical Transducermentioning

confidence: 99%

Advances and challenges in biosensor-based diagnosis of infectious diseases

Sin

Mach

Wong

et al. 2014

Expert Review of Molecular Diagnostics

333

238

View full text Add to dashboard Cite

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“…(D) Quantitative measurement of titer strength for syphilis in human sera samples. Ref 108–Reproduced by permission of The Royal Society of Chemistry, Copyright 2010. (E) Binding of trinitrophenol to its antibody using an end point assay.…”

Section: Figurementioning

confidence: 99%

Interferometric Methods for Label-Free Molecular Interaction Studies

2011

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Figure 7. (A) BSI shows the difference in binding affinities for free-solution and surface-immobilized DNA hybridization. 25 (B) BSI detects the kinetic interaction of calmodulin and trifluoperazine. From ref 106. Reprinted with permission from American Association for the Advancement of Science, Copyright 2007. (C) Shows that the magnitude of binding as detected by BSI for α-crystallin to different T4L lysozyme mutants. Reprinted from ref 109.

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“…End‐point assays were performed similarly to those recently described by our group 15. In short, the changes in RI arising from binding with CAII were measured for a series of inhibitor concentrations, allowing the determination of K D for the interaction.…”

Section: Concentrations Of Caii and Inhibitor Used On Each Analysis Wmentioning

confidence: 99%

“…Creating a separate DMSO calibration to subtract the bulk effects of DMSO alone is unnecessary with BSI, eliminating preparation error that could be present in other methodologies. BSI not only allows binding partners to remain in their native state without potentially perturbing labels or immobilizations, but also more easily accommodates assays that require DMSO, complex matrices 15 or other high‐RI additives. Finally, BSI is not a mass‐sensitive sensor making it compatible with a wide range of solutes and targets including small molecule–protein interactions, protein–DNA or small molecule–DNA interactions and large protein complex–inhibitor interactions.…”

Section: Concentrations Of Caii and Inhibitor Used On Each Analysis Wmentioning

confidence: 99%

Free‐solution interaction assay of carbonic anhydrase to its inhibitors using back‐scattering interferometry

et al. 2010

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Back-scattering interferometry (BSI) is a label-free, free-solution, small-volume technique used for characterizing binding interactions, which is also relevant to a growing number of biosensing applications including drug discovery. Here we use BSI to characterize the interaction of carbonic anhydrase (CAII) with five well known CAII inhibitors (±sulpiride, sulfanilamide, benzene sulfonamide, dansylamide, and acetazolamide) in the presence of dimethyl sulfoxide (DMSO). Dissociation constants (KD) calculated for each interaction were consistent with literature values previously obtained using surface plasmon resonance (SPR) and fluorescence-based competition assays. Results demonstrate the potential of BSI as a drug-screening tool which is fully-compatible with DMSO and does not require immobilization or labeling, therefore allowing binding interactions to be characterized in the native state. BSI has the potential for reducing labor costs, sample consumption and assay time while providing enhanced reliability over existing techniques.

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The potential of backscattering interferometry as an in vitro clinical diagnostic tool for the serological diagnosis of infectious disease

Abstract: Backscattering interferometry enables the detection of syphilis antibody–antigen interactions in the presence of human serum, showing promise as a diagnostic tool for the serological diagnosis of infectious disease with potentially quantitative capabilities.

Cited by 18 publications

References 14 publications

Advances and challenges in biosensor-based diagnosis of infectious diseases

Advances and challenges in biosensor-based diagnosis of infectious diseases

Interferometric Methods for Label-Free Molecular Interaction Studies

Free‐solution interaction assay of carbonic anhydrase to its inhibitors using back‐scattering interferometry

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