The potential of micelleplexes as a therapeutic strategy for osteosarcoma disease

Melim, Catarina; Jarak, Ivana; Veiga, Francisco; Figueiras, Ana

doi:10.1007/s13205-020-2142-5

Cited by 12 publications

(7 citation statements)

References 118 publications

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“…Osteosarcoma is one of the most common types of primary malignant cancer in bone tissues, primarily affecting adolescents and young adults (1). The majority of osteosarcomas occur in the metaphysis of the long bones of the extremities, whereas the involvement of the axial or the craniofacial bone can also be observed in the adult population (2).…”

Section: Introductionmentioning

confidence: 99%

Knockdown of KRT17 decreases osteosarcoma cell proliferation and the Warburg effect via the AKT/mTOR/HIF1α pathway

Yan

Yang

et al. 2020

Oncol Rep

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Keratins are fibrous structural proteins that serve essential roles in forming the stratum corneum and protect the cells in this layer of skin from damage. Keratin 17 (KRT17) is a key member of the keratins, and dysregulated expression of KRT17 has been reported in various types of cancer, such as lung and gastric cancer. The present study aimed to identify the role of KRT17 in osteosarcoma and the underlying molecular mechanism. The expression of KRT17 in osteosarcoma tissues and cell lines was detected using reverse transcription-quantitative PCR (RT-qPCR) and western blotting. The effects of KRT17 on osteosarcoma cell proliferation and the Warburg effect in vitro were detected using CCK-8 and colony formation assays, cell cycle distribution analysis and metabolic measures. The effects of KRT17 on osteosarcoma cell proliferation in vivo were detected using a subcutaneous tumorigenesis model. The association between KRT17 and the AKT/mTOR/hypoxia-inducible factor 1α (HIF1α) pathway was detected using RT-qPCR and western blotting. The results demonstrated that KRT17 was highly expressed in osteosarcoma tissues and cell lines. Knockdown of KRT17 decreased osteosarcoma cell proliferation and colony formation, induced G 1 phase arrest and inhibited glycolysis in vitro. Similarly, the suppression of KRT17 decreased osteosarcoma tumor growth in vivo. Knockdown of KRT17 decreased the expression of phosphorylated (p)-AKT, p-mTOR, HIF1α and the target gene of HIF1α glucose transporter 1. Restoring the expression of p-AKT, p-mTOR or HIF1α reversed the effect of KRT17 inhibition on cell proliferation and glycolysis. These results indicated that knockdown of KRT17 may be an effective method for treating osteosarcoma through inhibiting osteosarcoma cell proliferation and the Warburg effect by suppressing the AKT/mTOR/HIF1α pathway.

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Section: Introductionmentioning

confidence: 99%

Knockdown of KRT17 decreases osteosarcoma cell proliferation and the Warburg effect via the AKT/mTOR/HIF1α pathway

Yan

Yang

et al. 2020

Oncol Rep

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“…It was found that these nanoparticles contributed to the slow release of heparin and decreased the viability of OS cells (induction of DNA condensation, arrest in G1 phase), while increasing the uptake of BBR [ 263 ]. Therefore, heparin-based nanoparticles could be potential carriers for the treatment of OS [ 263 , 264 ].…”

Section: Prospects For Heparin-containing Medical Constructsmentioning

confidence: 99%

Heparin and Heparin-Based Drug Delivery Systems: Pleiotropic Molecular Effects at Multiple Drug Resistance of Osteosarcoma and Immune Cells

et al. 2022

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One of the main problems of modern health care is the growing number of oncological diseases both in the elderly and young population. Inadequately effective chemotherapy, which remains the main method of cancer control, is largely associated with the emergence of multidrug resistance in tumor cells. The search for new solutions to overcome the resistance of malignant cells to pharmacological agents is being actively pursued. Another serious problem is immunosuppression caused both by the tumor cells themselves and by antitumor drugs. Of great interest in this context is heparin, a biomolecule belonging to the class of glycosaminoglycans and possessing a broad spectrum of biological activity, including immunomodulatory and antitumor properties. In the context of the rapid development of the new field of “osteoimmunology,” which focuses on the collaboration of bone and immune cells, heparin and delivery systems based on it may be of intriguing importance for the oncotherapy of malignant bone tumors. Osteosarcoma is a rare but highly aggressive, chemoresistant malignant tumor that affects young adults and is characterized by constant recurrence and metastasis. This review describes the direct and immune-mediated regulatory effects of heparin and drug delivery systems based on it on the molecular mechanisms of (multiple) drug resistance in (onco) pathological conditions of bone tissue, especially osteosarcoma.

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“…As micellar shape and size are controllable, the drug loading capacity of micelles can be increased. The hydrophobic core of micelles allows the encapsulation of poorly soluble drugs, while the hydrophilic shell provides an opportunity for surface modification of the micelles to improve targeting (Melim et al, 2020).…”

Section: Micelles As Anti-osteosarcoma Nanocarriersmentioning

confidence: 99%

The Application of 3D-Printing and Nanotechnology for the Targeted Treatment of Osteosarcoma

et al. 2021

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Osteosarcoma is a malignant bone neoplasm prevalent in adolescents. Current therapies include chemotherapy and surgery. Surgical resection of osteosarcoma induces a large bone defect which may be overcome by employing scaffolds for bone tissue engineering. This review details the polymers and bioceramics that may be used to fabricate 3D printed scaffolds for bone regeneration and the nanotechnology strategies that may be incorporated into such scaffolds. Natural polymers discussed include chitosan, alginate, collagen, gelatin, and silk fibroin. Synthetic polymers discussed include polycaprolactone, polyurethane, poly(lactic)acid and poly(vinyl) alcohol. Bioceramics that are utilized in bone regeneration such as calcium phosphate, calcium silicate and bioglass are elaborated on. Furthermore, comparison data between different types of 3D printed scaffolds for bone regeneration are presented. A discussion on Photo-responsive and magneto-responsive 3D printed scaffolds that have been fabricated for bone regeneration is included. Research concerning drug-loaded scaffolds as well as the incorporation of nanocarriers into scaffolds for bone regeneration is provided. Chemotherapy utilized in osteosarcoma therapy has severe adverse effects due to being non-selective between healthy cells and tumor cells. A possible way to overcome this is to utilize nanotechnology. Therefore, research detailing other types of nanocarriers that have the potential to be incorporated into 3D printed scaffolds for localized adjuvant therapy is presented.

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The potential of micelleplexes as a therapeutic strategy for osteosarcoma disease

Cited by 12 publications

References 118 publications

Knockdown of KRT17 decreases osteosarcoma cell proliferation and the Warburg effect via the AKT/mTOR/HIF1α pathway

Knockdown of KRT17 decreases osteosarcoma cell proliferation and the Warburg effect via the AKT/mTOR/HIF1α pathway

Heparin and Heparin-Based Drug Delivery Systems: Pleiotropic Molecular Effects at Multiple Drug Resistance of Osteosarcoma and Immune Cells

The Application of 3D-Printing and Nanotechnology for the Targeted Treatment of Osteosarcoma

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