The Potential of miR-21 in Stem Cell Differentiation and its Application in Tissue Engineering and Regenerative Medicine

Kalhori, Mohammad Reza; Soleimani, Masoud; Alibakhshi, Reza; Kalhori, Amir Ali; Mohammadi, Parisa; Azreh, Rasoul; Farzaei, Mohammad Hosein

doi:10.1007/s12015-023-10510-8

Cited by 3 publications

(4 citation statements)

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“…Recently, it was demonstrated miRNAs, especially viral miRNAs, contribute to stemness in NPC and other cancers, as well as to immune escape, representing important biomarkers of malignant tumors 34,35 . It is known that miR‐21 inhibits stem cell pluripotency and self‐renewal and induces cell differentiation by targeting multiple genes 36 . Cai et al revealed a possible role for EBV‐miR‐BART7‐3p in imposing stemness of NPC 37 .…”

Section: Discussionmentioning

confidence: 99%

“… 34 , 35 It is known that miR‐21 inhibits stem cell pluripotency and self‐renewal and induces cell differentiation by targeting multiple genes. 36 Cai et al revealed a possible role for EBV‐miR‐BART7‐3p in imposing stemness of NPC. 37 Zeng et al demonstrated that the miR‐328‐3p‐CPT1A‐FAO axis plays a critical role in breast cancer metastasis by regulating breast cancer cell stemness.…”

Section: Discussionmentioning

confidence: 99%

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Targeting exosomes enveloped EBV‐miR‐BART1‐5p‐antagomiRs for NPC therapy through both anti‐vasculogenic mimicry and anti‐angiogenesis

et al. 2023

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Targeting exosomes enveloped EBV‐miR‐BART1‐5p‐antagomiRs for NPC therapy through both anti‐vasculogenic mimicry and anti‐angiogenesis

et al. 2023

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“…In naïve hESCs, there is robust expression of the miR-371-373 cluster [59,60], in addition to other identified markers such as miR-143-3p and miR-22-3p [59], suggesting that their absence in conventional hESCs are more likely due to TSC differentiation resulted in the significant upregulation of 25 miRNAs and significant downregulation of 18 miRNAs relative to controls [77]. The miRNA with the largest increase in expression was miR-21 [77], which is known to have roles in differentiation via targeting the transcripts of Pou5f1, Nanog, Sox2, and c-Myc [79][80][81]. Other miRNAs that increased in expression include members of the let-7 family, miR-467, and miR-466, while members of the miR-302/367 cluster were decreased (known to maintain hESC stemness [31,82], as discussed earlier) [77].…”

Section: Humanmentioning

confidence: 95%

Evidence for Functional Roles of MicroRNAs in Lineage Specification During Mouse and Human Preimplantation Development

Biondic,

Petropoulos

2023

Yale J Biol Med

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Proper formation of the blastocyst, including the specification of the first embryonic cellular lineages, is required to ensure healthy embryo development and can significantly impact the success of assisted reproductive technologies (ARTs). However, the regulatory role of microRNAs in early development, particularly in the context of preimplantation lineage specification, remains largely unknown. Taking a cross-species approach, this review aims to summarize the expression dynamics and functional significance of microRNAs in the differentiation and maintenance of lineage identity in both the mouse and the human. Findings are consolidated from studies conducted using in vitro embryonic stem cell models representing the epiblast, trophectoderm, and primitive endoderm lineages (modeled by naïve embryonic stem cells, trophoblast stem cells, and extraembryonic endoderm stem cells, respectively) to provide insight on what may be occurring in the embryo. Additionally, studies directly conducted in both mouse and human embryos are discussed, emphasizing similarities to the stem cell models and the gaps in our understanding, which will hopefully lead to further investigation of these areas. By unraveling the intricate mechanisms by which microRNAs regulate the specification and maintenance of cellular lineages in the blastocyst, we can leverage this knowledge to further optimize stem cell-based models such as the blastoids, enhance embryo competence, and develop methods of non-invasive embryo selection, which can potentially increase the success rates of assisted reproductive technologies and improve the experiences of those receiving fertility treatments.

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“…Moreover, having a crucial function in the neural development and physiology, miRNAs are found in large numbers in the CNS [17,18] . Recent studies have also shown that miRNAs participate in proliferation and differentiation of stem cells via regulating expression of many stem cell regulatory factors [19][20][21][22] . Doublecortin X (DCX) is broadly expressed in neural precursor cells and is a protein associated with microtubules after migration during mitosis and is a key protein involved in neuronal differentiation and a major marker of neural precursor cells [23] .…”

mentioning

confidence: 99%

The Impact of microRNA-29a-3p on the Neural Stem Cell Differentiation Process into Neurons

Shi,

Zhou,

Dai

et al. 2023

IJPS

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Shi et al.: microRNA-29a-3p Impact on the Neural Stem Cell DifferentiationTo explore how microRNA-29a-3p influences the differentiation of neural stem cells into neurons and to identify any potential mechanisms involved. The program TargetScan, available online was utilized to examine and make predictions about the microRNA-29a-3p located upstream that regulate doublecortin X messenger ribonucleic acid. Ultimately, microRNA-29a-3p was preferred as the main central point of the investigation. The targeting of doublecortin X gene by microRNA-29a-3p was validated by employing a dual-luciferase reporter gene system. Before subjecting to adherent differentiation, the neural stem cells were transfected with microRNA-29a-3p inhibitor, microRNA-29a-3p mimic and normal control. MicroRNA and doublecortin X messenger ribonucleic acid levels were measured through quantitative reverse transcriptionpolymerase chain reaction and doublecortin X protein levels were determined through Western blot assays. The differentiation of neuronal precursor cells was detected by doublecortin X immunofluorescence. Neuronal differentiation was detected by microtubule-associated protein 2 immunofluorescence. According to the dual-luciferase reporter gene system, miR-29a-3p targeted the doublecortin X gene; microRNA-29a-3p significantly decreased in differentiated cells for 3 d was observed, along with a significant reduction in levels of doublecortin X protein and messenger ribonucleic acid. On the other hand, a significant raise in microRNA-29a-3p in differentiated cells of microRNA-29a-3p mimic group for a period of 3 d was noticed with a significant drop in protein levels, doublecortin X messenger ribonucleic acid and doublecortin X immunofluorescence-positive neuronal precursor cells. From the microtubule-associated protein 2 immunofluorescence, it was confirmed that microRNA-29a-3p inhibitor group significantly elevated the total number of neurons, while microRNA-29a-3p mimic reduced the neurons on 7 d. Therefore, low expression of microRNA-29a-3p promote differentiation of neural stem cells into neurons via targeting doublecortin X.

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The Potential of miR-21 in Stem Cell Differentiation and its Application in Tissue Engineering and Regenerative Medicine

Cited by 3 publications

References 165 publications

Targeting exosomes enveloped EBV‐miR‐BART1‐5p‐antagomiRs for NPC therapy through both anti‐vasculogenic mimicry and anti‐angiogenesis

Targeting exosomes enveloped EBV‐miR‐BART1‐5p‐antagomiRs for NPC therapy through both anti‐vasculogenic mimicry and anti‐angiogenesis

Evidence for Functional Roles of MicroRNAs in Lineage Specification During Mouse and Human Preimplantation Development

The Impact of microRNA-29a-3p on the Neural Stem Cell Differentiation Process into Neurons

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