The Potential Use of Stem Cells for Cochlear Repair

Parker, Marcie; Cotanche, Douglas A.

doi:10.1159/000075998

Cited by 39 publications

(21 citation statements)

References 74 publications

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“…To properly investigate this possibility, however, requires an independent method for determination of the cause of the hearing loss. When more direct biological treatments become available (e.g., Parker & Cotanche, 2004), it will become even more important to be able to determine the underlying cellular system primarily responsible for the hearing loss. Clearly, it would be unproductive and possibly dangerous to introduce treatments aimed at regeneration or repair of hair cells if the major cause of hearing dysfunction in that individual was not hair cell dysfunction, but a decline of endocochlear potential (EP) caused by degeneration of the stria vascularis.…”

Section: Earlier Attempts At Differential Diagnosis Of Sensorineural mentioning

confidence: 99%

Determining the Cause of Hearing Loss: Differential Diagnosis Using a Comparison of Audiometric and Otoacoustic Emission Responses

Mills

2006

Ear &Amp; Hearing

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The results clearly demonstrate the possibility of developing a clinical method of noninvasive differential diagnosis of hearing loss. The method demonstrated was to add to a standard audiometric evaluation the measurement of DPOAE growth functions over the range of frequencies where these emissions were relatively easy to measure and consistent. The DPOAE stimulus frequencies were chosen to match the audiometric frequencies, and the corresponding emission and audiometric thresholds were compared on a threshold-threshold plot for each individual at a number of stimulus frequencies. Responses in different regions in this plot were found to correspond to different types of sensorineural hearing loss.

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Section: Earlier Attempts At Differential Diagnosis Of Sensorineural mentioning

confidence: 99%

Determining the Cause of Hearing Loss: Differential Diagnosis Using a Comparison of Audiometric and Otoacoustic Emission Responses

Mills

2006

Ear &Amp; Hearing

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“…Another method of cellular replacement is the therapeutic application of exogenous cells that are capable of developing into cochlear cell types. The development of multipotent cell lines derived from stem cells represents a practical source of exogenous cells for the replacement of damaged or ablated cochlear cells (Parker and Cotanche, 2004).…”

Section: Introductionmentioning

confidence: 99%

Neural stem cells injected into the sound-damaged cochlea migrate throughout the cochlea and express markers of hair cells, supporting cells, and spiral ganglion cells

Parker

Corliss²,

Gray³

et al. 2007

Hearing Research

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Most cases of hearing loss are caused by the death or dysfunction of one of the many cochlear cell types. We examined whether cells from a neural stem cell line could replace cochlear cell types lost after exposure to intense noise. For this purpose, we transplanted a clonal stem cell line into the scala tympani of sound damaged mice and guinea pigs. Utilizing morphological, protein expression and genetic criteria, stem cells were found with characteristics of both neural tissues (satellite, spiral ganglion, and Schwann cells) and cells of the organ of Corti (hair cells, supporting cells). Additionally, noise-exposed, stem cell-injected animals exhibited a small but significant increase in the number of satellite cells and Type I spiral ganglion neurons compared to non-injected noise-exposed animals. These results indicate that cells of this neural stem cell line migrate from the scala tympani to Rosenthal's canal and the organ of Corti. Moreover, they suggest that cells of this neural stem cell line may derive some information needed from the microenvironment of the cochlea to differentiate into replacement cells in the cochlea.

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“…After each 5-day interval, cells were in need of feeding with the same concentration and composition of supplements as the last cultured cells AP staining-based characterization of placental membrane cells in in vitro bubaline species. Even though stem cells and their potential applications have emerged as a potential oasis in the field of disease treatment, there is a difference of opinion among scientists even in the definition of the term stem cell, which is still clearly an evolving concept (Parker and Cotanche 2004). The main characteristic of stem cells is their pluripotency and their self-regeneration capacity, properties conferred by the presence of some cell transcription factors, of which the most studied are Oct-3/4 and Nanog.…”

Section: Discussionmentioning

confidence: 99%

Expression of Transcriptional Factor Genes (Oct-4, Nanog, and Sox-2) and Embryonic Stem Cell-Like Characters in Placental Membrane of Buffalo (Bubalus bubalis)

et al. 2012

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The aim of the study was to assess the expression of transcriptional factor genes and embryonic stem cell-like characters in the placental membrane of buffalo (Bubalus bubalis). Along with the placenta, amniotic fluid, maternal peripheral blood, and umbilical cord blood samples were taken for the future study. The isolation and culture of cells from the placental membrane was followed by the determination of RT-PCR-based markers (Oct-4, Nanog, Sox-2, alkaline phosphatase, stem cell factor, and Nestin) of these cells. Placental membrane cells also positively expressed alkaline phosphatase staining. We isolated adherent cells from trypsin-EDTA-digested placentas and examined these cells for morphology, surface markers, and differentiation potential and found that they expressed several stem cell markers. They also showed neurogenic and adipogenic differentiation potentials under appropriate guided conditions. We suggest that placenta-derived cells have multilineage differentiation potential similar to mesenchymal stem cells in terms of morphology and cell-surface antigen expression. The placenta may prove to be a useful source of mesenchymal stem cells.

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The Potential Use of Stem Cells for Cochlear Repair

Cited by 39 publications

References 74 publications

Determining the Cause of Hearing Loss: Differential Diagnosis Using a Comparison of Audiometric and Otoacoustic Emission Responses

Determining the Cause of Hearing Loss: Differential Diagnosis Using a Comparison of Audiometric and Otoacoustic Emission Responses

Neural stem cells injected into the sound-damaged cochlea migrate throughout the cochlea and express markers of hair cells, supporting cells, and spiral ganglion cells

Expression of Transcriptional Factor Genes (Oct-4, Nanog, and Sox-2) and Embryonic Stem Cell-Like Characters in Placental Membrane of Buffalo (Bubalus bubalis)

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