The practical angler / by Piscator [i.e. W. Hughes].

Some experiments on the disintegration of yeast by high intensity ultrasound

“…Whole cells were counted for treated samples and untreated controls. This was time consuming and eyestrain of the okserver was a limiting factor;1Y (2) Whole cells can be separated from the broken cell remains by centrifuging and the proportion of the original mixture e~timated;'~ (3) After centrifuging whole cells and debris. the total nitrogen released into the medium was determined by a micro-Kjeldahl method, and compared with the total nitrogen content of the cells;…”

“…The various suggestions may be summarized as follows: (2) Shearing forces due to microstreaming of the cell fluid.2s8 (J) Pressures and pressure gradients resulting from the collapse of 247 gas I)iiIhlt~s which r o m~ orit of solution on or near thc cell wall. Darnagc~ may rwult, from a siiiglr eveiit or fatigue, iiivolving a threshold time.…”

Effects of Steroid‐Induced Pneumocystis carinii on Alveolar Macrophages in the Rat

“…In addition to tetracycline, the group called Pc rats received 25 mg cortisone acetate (Merck, Sharpe, and Dohme) twice weekly as in [ 11. The third group (Pc controls) received the same dose of cortisone plus trimethoprim-sulfamethoxazole (TMP-SMZ) (Burroughs Wellcome Co.) as in [3]. The Pc rats developed Pneumocystis infections and their lungs were packed with uophozoites and cysts by 6-8 wk of steroid treatment, as detected with Din-Quick (Harleco).…”

Circulating antigen of Aspergillus fumigatus in cortisone-treated mice challenged with conidia: Detection by counterimmunoelectrophoresis

“…An increasing incidence of invasive asper~losis has been encounterer', in recipients of transplanted organs [1][2][3], open-heart surgery patients [4,5] and other compromised hosts [6][7][8]. Without early diagnosis and treatment the infection is often fatal.…”