The Prenatal Development of the Dog: Preimplantation Events1

Holst, P. A.; Phemister, Robert D.

doi:10.1093/biolreprod/5.2.194

Cited by 145 publications

(93 citation statements)

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“…Unique species-specific reproductive characteristics substantially affect development of ARTs in dogs. In contrast with most mammalian species, in which the oocyte completes its first meiotic division in the preovulatory follicle and matured oocytes are ovulated and ready for fertilization within the oviduct, the domestic bitch ovulates immature oocytes at the germinal vesicle stage and the oocytes undergo a 48-72-h period of postovulatory maturation in the upper regions of the oviduct, and during oocyte maturation in vivo oocytes are exposed to an ever-changing environment of gonadotropins, steroids, growth factors, and many other factors, any or all of which may interact to regulate maturational changes that occur in the oocyte and its surrounding cumulus cells during the preovulatory period [1][2][3][4][5][6][7]. Clearly, these factors are assumed to be beneficial and involved in nuclear and/or cytoplasmic maturation of oocytes during IVM for several mammalian species, for example porcine [8,9], human [10], mouse [11], equine [12], and bovine [13].…”

Section: Introductionmentioning

confidence: 99%

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Abdel-Ghani

Abe

Asano

et al. 2010

Reprod Medicine & Biology

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Purpose To investigate the ability of medium conditioned with bovine cumulus-oocyte complexes (COCs) to support nuclear maturation of canine oocytes recovered from domestic dog ovaries. Methods Cumulus-oocyte complexes were obtained from ovaries of domestic bitches (8 months old to 7 years old), and in-vitro maturation was evaluated in TCM-199 supplemented with different concentrations (0, 20, 30 or 50%) of bovine COCs-conditioned medium (BCM). The canine COCs were cultured for 72 or 96 h at 38.5°C in 5% CO 2 , 5% O 2 and 90% N 2 . The bovine COCs-conditioned medium was obtained from culture of bovine COCs with TCM-199 supplemented with 5% FCS for 22 h at 38.5°C in 2% CO 2 , 98% air. Results The proportion of germinal vesicle breakdown (GVBD) after 72 h was significantly higher (P \ 0.05) in medium supplemented with 30% BCM (20.7%) compared with the control group (13.4%). The rates of GVBD-MII stage were significantly higher (P \ 0.05) when oocytes were matured with BCM at concentration of 30% (41.5%) compared with control (26.6%) after 72 h in-vitro culture. After 96 h in-vitro culture, the oocytes matured in medium supplemented with 30% BCM (5.5%) showed a significant increase (P \ 0.05) in the proportion of MII compared with control (0.7%). However, increasing the cultivation time from 72 to 96 h resulted in an increase in oocyte degeneration rate. Conclusions The results suggested that bovine COCsconditioned medium supplementation significantly increased nuclear maturation of canine oocytes.

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Section: Introductionmentioning

confidence: 99%

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Abdel-Ghani

Abe

Asano

et al. 2010

Reprod Medicine & Biology

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“…However, due to the particular physiology of the canine female, progress in this area has been relatively slow compared with other species. Canine oocytes are ovulated as immature oocytes at 632 T. Otoi et al the germinal vesicle stage and require 2 to 5 days for the completion of meiotic maturation within the oviducts [1,2]. Moreover, spermatozoa are already present around ovulated oocytes before the completion of meiotic maturation, because ovulation occurs approximately 2 to 3 days after the onset of oestrus, during which bitches are receptive to mating before ovulation [3].…”

Section: Introductionmentioning

confidence: 99%

Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization

et al. 2004

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-The objective of this study was to determine an optimum maturation period of canine oocytes for the development in vitro after in vitro fertilization (IVF). Canine oocytes larger than 110 micrometers in diameter, which were collected from ovaries at the follicular phase of the reproductive cycle, were cultured for each time (48, 72 and 96 h) in TCM 199 medium supplemented with 10% canine serum, fertilized, and then cultured in vitro for 8 days. Significantly more oocytes reached metaphase II (MII) in the 72-h culture group than in the 48-h culture group (25.6% vs. 41.0%). The percentages of oocytes that reached MII or beyond after maturation culture did not differ significantly between the 72-and 96-h culture groups, but the percentage of parthenogenetically activated oocytes in the 96-h culture group was significantly higher than that in the 72-h culture group. The percentages of cleaved embryos after IVF were significantly higher in the 48-and 72-h culture groups than in the 96-h culture group. In the 48-h culture group, 3.9% of fertilized oocytes developed to the 16-cell stage or beyond, but none of the cleaved embryos in the 72-and 96-h culture groups developed to the same stage. These results indicate that full nuclear maturation of oocytes collected from ovaries at the follicular phase occurs after 72 h of in vitro culture. However, an optimum maturation period (48 h) for the in vitro development of canine oocytes after IVF may be different from the period necessary to reach the maximal oocyte maturation rate, when based on the developmental stage of the cleaved embryos.developmental competence / incubation duration / meiotic maturation / oestrous stage

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“…The first easily observable change during a dog implantation is when the blastocysts reach the definitive position in the uterus and the endometrial edema undergoes morphological changes that indicate the position. When the blastocyst localized the endometrial endema, the embryos are hatched from the zona pellucida and the embryonic disc is differentiated into the primitive streak (Holst and Phemister 1971). Contrary to these observations, Gier (1950) suggested that the embryos are still in a free-floating blastocyst stage prior to the formation of somites.…”

Section: Discussionmentioning

confidence: 99%

Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period

Bukowska¹,

Kempisty²,

Jackowska³

et al. 2011

Polish Journal of Veterinary Sciences

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Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs.All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR.In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001).Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.

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The Prenatal Development of the Dog: Preimplantation Events1

Cited by 145 publications

References 0 publications

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization

Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period

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