The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I

Abstract

Constructing biomimetic structure and incorporating bioactive molecules is an effective strategy to achieve a more favorable cell response. To explore the effect of electrospinning (ES) nanofibrous architecture and collagen I (COL I)-incorporated modification on tuning osteoblast response, a resorbable membrane composed of poly(lactic-co-glycolic acid)/poly(caprolactone) (PLGA/PCL; 7:3 w/w) was developed via ES. COL I was blended into PLGA/PCL solution to prepare composite ES membrane. Notably, relatively better cell response was delivered by the bioactive ES-based membrane which was fabricated by modification of 3,4-dihydroxyphenylalanine and COL I. After investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement, and mechanical test, polyporous three-dimensional nanofibrous structure with low tensile force and the successful integration of COL I was obtained by the ES method. Compared with traditional PLGA/PCL membrane, the surface hydrophilicity of collagen-incorporated membranes was largely enhanced. The behavior of mouse preosteoblast MC3T3-E1 cell infiltration and proliferation on membranes was studied at 24 and 48 hours. The negative control was fabricated by solvent casting. Evaluation of cell adhesion and morphology demonstrated that all the ES membranes were more favorable for promoting the cell adhesion and spreading than the casting membrane. Cell Counting Kit-8 assays revealed that biomimetic architecture, surface topography, and bioactive properties of membranes were favorable for cell growth. Analysis of β1 integrin expression level by immunofluorescence indicated that such biomimetic architecture, especially COL I-grafted surface, plays a key role in cell adhesion and proliferation. The real-time polymerase chain reaction suggested that both surface topography and bioactive properties could facilitate the cell adhesion. The combined effect of biomimetic architecture with enhanced surface activity by 3,4-dihydroxyphenylalanine-assisted modification and COL I incorporation of PLGA/PCL electrospun membranes could successfully fill osteogenic defects and allow for better cell proliferation and differentiation.

Design and optimization of biocompatible polycaprolactone/poly (l‐lactic‐co‐glycolic acid) scaffolds with and without microgrooves for tissue engineering applications

“…This hypothesis explains the increased capacity of fibroblasts to adhere to pure PLGA and the PCL70/PLGA30 smooth membranes, as observed either by the SEM or fluorescence microscopy results. The literature shows that hydrophilic surfaces facilitates adhesion and proliferation of cells on the scaffolds . The sharp changes in topology and surface roughness can have a direct effect on contact angles, as well as cell—surface interactions of the scaffolds .…”

“…The literature shows that hydrophilic surfaces facilitates adhesion and proliferation of cells on the scaffolds. [47][48][49] The sharp changes in topology and surface roughness can have a direct effect on contact angles, as well as cell-surface interactions of the scaffolds. 50,51 Despite the slight increase in the contact angle found for PLGA membranes, this does not completely explain the good results found for the PCL70/PLGA30 blend.…”

In Vitro and In Vivo Evaluation of a Polycaprolactone (PCL)/Polylactic-Co-Glycolic Acid (PLGA) (80:20) Scaffold for Improved Treatment of Chondral (Cartilage) Injuries

“…The International Standard ISO 10993 (Biological Evaluation of Medical Devices) recommends that materials used in humans must be subjected to in vitro and biocompatibility assays to verify the response of cells interacting with them [ 45 ]. According to different studies, the viability that allows the PCL and PLGA scaffolds in isolation and together (PCL/PLGA) is favorable, indicating that they have low toxicity and adequate interaction with biological systems [ 22 , 46 , 47 ]. Our results showed no cytotoxic effects in the groups evaluated (PCL/PLGA scaffold + DFMSCs cultured in DMEM and PCL/PLGA scaffold + DFMSCs cultured with chondrogenic medium) even on 21 and 28 days, with increased activity of cell metabolism, which translates into greater viability and proliferation above 100% ( Figure 2 ).…”

A Novel Method for Fabricating the Undulating Structures at Dermal—Epidermal Junction by Composite Molding Process

“…In this study, the hydrophilicity of PLGA-PCL nanofiber membranes was evaluated using four different concentration ratios, and the findings were illustrated in Figure 3C, which indicated that 15%PLGA nanofiber membranes exhibited the highest hydrophilicity, while the hydrophilicity of electrospinning PLGA-PCL membranes diminished as the concentration of PCL increased in the electrospinning solution. This decline was due to the hydrophobic characteristics of PCL [37,38].…”