The Preparation and Characterization of Lyophilized Polyacrylamide Enzyme Gels for Chemical Analysis.

Hicks, G. P.; Updike, Stuart J.

doi:10.1021/ac60238a014

Cited by 184 publications

(34 citation statements)

References 24 publications

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“…Hicks and Updike fabricated the biosensor, dextrose enzyme pole, in 1966. 1 Up to the 1970s, the research domain of the biosensors emerged. After that, the biosensor-based research, e.g., enzyme sensor, flourished gradually.…”

Section: Introductionmentioning

confidence: 99%

Hybrid metallic nanoparticles for excitation of surface plasmon resonance

Zhu

Luo

et al. 2007

Journal of Applied Physics

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A Ag nanostructure was put forward in this paper. There are two types of Ag nanoparticles, spherical and pyramidal particles. Both of them have the same period, but different height and shapes. The hybrid nanoparticles can produce the localized surface plasmon resonance ͑LSPR͒, which couples each other and leads to an extra peak transmission. Our UV-visible-IR spectrophotometer measurement results show that some extra small and sharp peaks appear besides the normal LSPR wave peaks in the transmittance spectrum. The hybrid Ag nanoparticles being used as nanosensors will be more sensitive and selective than the conventional LSPR-based nanosensors.

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Section: Introductionmentioning

confidence: 99%

Hybrid metallic nanoparticles for excitation of surface plasmon resonance

Zhu

Luo

et al. 2007

Journal of Applied Physics

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“…Another widely used procedure involves covalent coupling of enzymes to glass [22]. (For further reading see in particular H. Weetall in [20] [23,24] or of intact microbial cells [24], usually by polymerisation of enzyme-acrytamide mixtures, is another widely employed technique. Prior to polymerisation the enzyme is dissolved in a solution of acrylamide and cross-linking agents such as (bis)-acrylamide.…”

mentioning

confidence: 99%

Immobilised enzymes

Mosbach¹

1976

FEBS Letters

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“…The enzyme asparaginase (Merck Inc.) was immobilized in polyacrylamide gel [32] as follows. 30 g of polyacrylamide and 3 g of IV.lV'-methylene-bisacrylamide were dissolved in 100 ml of 0.1 M, pH 7.4, phosphate buffer.…”

Section: Methodsmentioning

confidence: 99%

Gel Entrapment of Enzyme

Nadler¹,

Updike²

1974

Enzyme

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The feasibility of gel entrapment of enzymes as a strategy for enzyme correction was tested using polyacrylamide gel containing the antitumor enzyme, asparaginase. Intravenous and/or intraperitoneal injections of labeled enzyme particles into rats resulted in a significant though transient reduction in serum asparagine levels. Cultivated human fibroblasts grown in the presence of particles failed to incorporate significant amounts of labeled gel or enzyme. In contrast, when particles were given intravenously, intracellular localization in the reticuloendothelial system could be demonstrated. These studies raise serious questions as to the potential usefulness of this approach for treatment of inborn errors of metabolism.

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The Preparation and Characterization of Lyophilized Polyacrylamide Enzyme Gels for Chemical Analysis.

Cited by 184 publications

References 24 publications

Hybrid metallic nanoparticles for excitation of surface plasmon resonance

Hybrid metallic nanoparticles for excitation of surface plasmon resonance

Immobilised enzymes

Gel Entrapment of Enzyme

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