The preparation and properties of two new chromogenic substrates of trypsin

Oxidant‐mediated proteolytic activation of Ca2+‐ATPase in microsomes of pulmonary smooth muscle

“…Vitamin E (1 mM) or aprotinin (1 mg/ml) was added for 15 min followed by treatment with H207 (1 mM) for 15 rain then Ca2+-ATPase activity was measured. Protease activity was assessed by determining hydrolysis of the synthetic substrate N-benzoyl-DL-arginine p-nitroanilide (BAPNA) by following the method previously described [24]. To determine the effect of H202, the microsomes were treated with 1 mM H202 for 15 min then protease activity was determined.…”

Preparation and properties of organophilic trypsin macro‐inhibitors: Diamidino‐α,ω‐diphenylcarbamyl‐poly(ethylene glycol)

“…Trypsin activity was determined according to Erlanger et al [5] and Mares-Guia and Shaw [6] using DL-BAPNA as substrate. After addition of the enzyme to the sample cuvette, the absorbance changes were recorded at 410 nm for 3-4 min; the slopes of the straight lines obtained gave the initial rates of reaction.…”

Isolation and Amino Acid Sequences of Two Trypsin Inhibitors from the Seeds of Bitter Gourd (Momordica charantia)

“…Trypsin inhibitory activity was assayed by a modification of the method of Erlanger et al 5) One hundred ttl of trypsin t This work was supported in part by a Grant-in-Aid for Scientific Research from Ministry of Education, Science, and Culture of Japan.…”