Preparation and properties of organophilic trypsin macro‐inhibitors: Diamidino‐α,ω‐diphenylcarbamyl‐poly(ethylene glycol)

Partition of trypsin in two‐phase systems containing a diamidino‐α, ω‐diphenylcarbamyl poly (ethylene glycol) as competitive inhibitor of trypsin

“…Poly(ethylene glycol) (PEG 9000;3~t w = 9000) was obtained from Fluka. Diamidino-~,w-diphenylcarbamyl-poly (ethylene glycol) (PEGPAB); Mw ~ 9000) was prepared as described earlier [5] (diamidino-a,co,-diphenylcarbamyl-poly (ethylene glycol). Trypsin, chymotrypsin, DL-benzoylargininep-nitroanilide (BAPNA) and N-acetyl-L-tyrosine ethyl ester (ATEE) were obtained from Sigma.…”

“…System (B) was obtained as system (A) but with PEG/PEGPAB (3:1). This ratio was selected to obtain full complexation of trypsin in regard to K i value of PEG 9000 PAB (2.0 10 -5 M [5]) and concentration of trypsin in the system (10 -4 M). Increasing concentration of PEGPAB is not useful since solubility of trypsin in the upper phase is limited.…”

“…As a model, p-aminobenzamidine (PAB), a strong inhibitor of trypsin, was attached to PEG and new macro-inhibitors of trypsin were obtained [5 ]. We…”