The Preparation and Properties of Mammalian Ribonucleic Acids<sup>1</sup>

Volkin, Elliot; Carter, C. E.

doi:10.1021/ja01148a032

Cited by 90 publications

(16 citation statements)

References 4 publications

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“…It would appear that as the RNA is quite stable after the heating process, the ribonuclease present is removed with the flocculated protein. The molecular weight of the product is similar to that of the preparation of Kay & Dounce (1953) and Volkin & Carter (1951). The higher molecular weights found by Grinnan & Mosher (1951) are not obtained.…”

Section: Discussionsupporting

confidence: 74%

The preparation and properties of ribonucleic acids from rat liver

Pain

Butler

1957

Biochemical Journal

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X-RAY DATA FOR MELEZITOSE 299 (A= 1F79OA) on a camera of diameter 114-6 mm. The principal diffraction lines measured, together with their intensities, are summarized in Tables 1 and 2. Oscillation and Weissenberg photographs of a single crystal of the monohydrate showed the crystal to be orthorhombic, and together with powder data the unit cell is calculated to be a=19-62+0-20, b=15*54+0-16, c=7-56+0-07k. The assignment of four molecules to the unit cell gives a calculated density of 1F505 g./cm.3 compared with a measured density of 1 51 g./cm.3 (pycnometer method). X-Ray powder diffraction lines of the dihydrate were indexed on the basis of an orthorhombic cell with dimensions a= 1980±0-20, b= 1565±0 16, c = 7*51 + 0 07 'A. The observed density (by flotation method) of 1-55 g./cm.3 (cf. Pionchon, 1897) agrees with the calculated value of 1-54 g./cm.3. The permission of the Ministry of Fuel and Power to publish this note is gratefully acknowledged.

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Section: Discussionsupporting

confidence: 74%

The preparation and properties of ribonucleic acids from rat liver

Pain

Butler

1957

Biochemical Journal

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“…Separation of the hexose phosphates was effected by forination of the borate complexes according to the illethod of Khym and Cohn (7). T h e pentose phosphates were reilloved according t o the method of Volkin and Carter (8). Separation of the adenine nucleotides was based on the work of several laboratories (9)(10)(11)(12).…”

Section: Separation Of the Various P Coinpounds Was Perfornled By Inementioning

confidence: 99%

Influences of Thyroxine and Insulin on the Turnover and Fate of High-Energy Phosphate

Dow¹

1964

Can. J. Biochem.

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Ingestion by the weanling rat of thyroxine in a n amount greatly in excess of physiological requirements resulted in significant increases in the rates of turnover of the high-energy compounds phosphocreatine (PC), adenosine diphosphate (ADP), and adenosine triphosphate (ATP) and in significant decreases in their concentrations in skeletal muscle. Nucleoside phosphorylase and myokinase activities and rates of pyrophosphate splitting of ATP and of utilization of ATE' for nuclear syntheses were unaltered in thyrotoxic muscle. The lindings suggest that increased extramitochondrial utilization of A'TP for glycolysis may be the factor responsible for the increased phosphorylating capacity of the thyrotoxic animal. An effect of thyroxine on mitochondrial permeability as well as on rate of formation of the high-energy intermediate would appear to explain the observed results.A further enhancement of the already markedly elevated thyrotoxic rate of phosphorylation was effected by the superimposition of insulin. Insulin also effected increases in the concentrations of the high-energy compounds. I t is apparent that insulin acts by reducing the utilization of ATP for phosphorylation of glucose and thus increases the ~liitocho~ldrial availability of high-energy acceptor. This increased mitocholldrial affinity for high-energy compounds results in a n enhancement of mitochondrial energy transfer processes.The findings appear t o i~ivolve both thyroxine and insuliu in the control of cell structure.

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“…The eluate was centrifuged to remove cellulose fibres. The optical density of the eluate was then measured at the appropriate wavelength (adenylic acid, 260 mp; guanylic acid, 260 mp (Volkin & Carter, 1951); cytidylic acid, 278 mp; uridylic acid, 262 mp (Ploeser & Loring, 1949)), and the molar concentrations of the nucleotides in the eluates calculated from the optical density measurements by using the appropriate millimolar extinction coefficients. Controls of adenylic acid, guanylic acid, cytid ylic acid and uridylic acid were run with each electrophoretogram.…”

Section: F Ahmad a H Rose And N K Gargmentioning

confidence: 99%