The Preparation of Aortic-Valve Homografts

Longmore, D. B.; Lockey, Eunice; Ross, Donald N.; Pickering, B N

doi:10.1016/s0140-6736(66)92770-x

Cited by 44 publications

(17 citation statements)

References 3 publications

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“…These were obtained from necropsy specimens and complied with the criteria used in the selection of homografts (Longmore et al, 1966). The valves were opened by cutting the aorta along the commissure between the left and right coronary cusps.…”

Section: Methodsmentioning

confidence: 99%

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A comparative study of different methods of processing aortic homografts

Welch¹

1969

Thorax

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Section: Methodsmentioning

confidence: 99%

“…Ethylene oxide sterilization, freeze-drying, and storage at room temperature (Longmore et al, 1966); 2. Ethylene oxide sterilization, flash-freezing in Cardice and acetone with storage at -79' (Ross, 1967 3.…”

Section: Methodsmentioning

confidence: 99%

A comparative study of different methods of processing aortic homografts

Welch¹

1969

Thorax

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“…Consequently, some of the procured cardiovascular tissues arriving in the Tissue Establishments are primarily contaminated [8]. Earlier methods used for allograft decontamination were harmful for allograft integrity [14][15][16][17][18][19].…”

Section: Discussionmentioning

confidence: 99%

Determination of Residual Antibiotics in Cryopreserved Heart Valve Allografts

Jashari

Faucon²,

Hoeck

et al. 2011

Transfus Med Hemother

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Introduction: Cardiovascular allografts are systematically incubated in antibiotics for their decontamination, and the antibiotics are removed before allograft implantation. We studied the occurrence of antibiotic residues in allograft valves. Methods: 12 experimental allografts were analyzed in this study. The concentration of the residual antibiotics was determined by high-performance liquid chromatography and the results were expressed as microgram per gram of allograft tissue. Results: The initial analysis showed that only vancomycin HCl and lincomycin HCl were retained in the allograft, whereas no traces of polymyxin B sulfate were detected in the tissue samples. Furthermore, the values found for the antibiotic residues in the extracted solution from the allografts were similar to the initial results: Vancomycin and lincomycin were detected in very low concentrations and no polymyxin B residues were observed. According to the World Health Organization (WHO), the maximum daily doses for vancomycin, lincomycin and polymyxin B are 2.0, 1.8 and 0.4 g, respectively. The thresholds for reporting degradation products are 0.05% for vancomycin and lincomycin and 0.1% for polymyxin B. The residual values for the two detected antibiotics were largely below 0.05%. Conclusions: Antibiotic residues in the allograft valves do not present any risk for their recipients. Increased allograft resistance to infections is probably due to the retention of antibiotics in the tissue.

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“…The first methods used, were more aggressive to valve tissues included ethylene oxide, gamma irradiation, beta-propiolactone, lyophilization but were soon discontinued as the clinical results demonstrated a reduction in their durability when compared with fresh grafts [2][3][4]. Analysis of the long-term results using fresh homografts created the concept that the durability was directly related to the degree of cellular viability of the tissue at implantation [5].…”

Section: Introductionmentioning

confidence: 99%

Análise das atividades dos oito anos iniciais do Banco de Valvas Cardíacas Humanas do Hospital de Caridade da Irmandade da Santa Casa de Misericórdia de Curitiba

Costa¹,

Costa²,

Nazareno³

et al. 2005

Rev Bras Cir Cardiovasc

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Objective: The aim of this study was to retrospectively evaluate the first eight years of activities of the Human Heart Valve Bank of Hospital de Caridade da Irmandade da Santa Casa de Curitiba (BVCHSC), analyzing aspects related to procurement, processing, storage and distribution of homologous cardiovascular grafts.Method: Initial donor screening was achieved according the national guidelines for human organ procurement, besides specific BVCHSC criteria. Hearts were obtained from multiple organ donors, non-beating heart donors and heart transplant recipients, with total ischemic times under 48 hours. Donor ages varied from neonates up to 60 years for aortic valves and 65 years for pulmonary valves. The dissected grafts had their dimensions measured and their morphology evaluated, using a classification system with category 0 (discarded), 1 (minimal morphological alterations) and 2 (perfect). The incidence and microorganisms responsible for organ contamination were determined, as was the efficiency of the decontamination solution. Aspects related to graft distribution were also analysed. Results: From September 1996 to February 2005, 1059hearts from 19 Brazilian states were received at BVCHSC. From these, 977 (92.3%) were from brain-death donors. A total of 2105 grafts were processed and of the aortic and pulmonary grafts that were analysed, 783 were in category 2, 697 in category 1 and 186 were discarded due to morphological abnormalities. In total, 433 received grafts were contaminated and the sterilization solution was efficient in 330 cases. 571 (27.1%) grafts were rejected during some phase of the processing, mainly due to contamination and morphological abnormalities. A total of 1338 grafts were distributed to 74 health institutions and were more commonly used for aortic valve replacement (529), correction of congenital heart diseases (478) and during the Ross procedure (272).Conclusions: The activities of the BVCHSC during the initial eight years were satisfactory, fulfilling the proposed goals.Descriptors: Tissue banks. Transplantation, homologous. Cryopreservation. Tissue preservation. 399COSTA Cardiovasc Surg 2005; 20(4) , MTBA ET AL -Analysis of the inicial eight years of activities of the Human Heart Valve Bank of the Hospital de Caridade da Irmandade da Santa Casa de Misericórdia de Curitiba Braz J

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The Preparation of Aortic-Valve Homografts

Cited by 44 publications

References 3 publications

A comparative study of different methods of processing aortic homografts

A comparative study of different methods of processing aortic homografts

Determination of Residual Antibiotics in Cryopreserved Heart Valve Allografts

Análise das atividades dos oito anos iniciais do Banco de Valvas Cardíacas Humanas do Hospital de Caridade da Irmandade da Santa Casa de Misericórdia de Curitiba

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