The preparation of autologous fibronectin for use in periodontal surgery

Abstract

A simple method for the preparation of autologous fibronectin and its subsequent use in periodontal surgery is presented. Fibronectin was isolated from autologous plasma using gelatine mediated affinity chromatography which afforded a yield of 76.5 ± 3.3 per cent. The fibronectin was concentrated to 5 mg/mL to produce a viscosity suitable for use in periodontal surgery. The purity and stability of the fibronectin was assessed by SDS polyacrylamide gel electrophoresis. It was found to contain no contaminants and to remain relatively stable at 4°C over a four to five‐week period.

The use of autologous fibronectin in the surgical repair of through‐and‐through furcation lesions in man A pilot study

“…The purity of the prepared fibroneetin was assessed using SDS-PAGE as described by Thompson et al (1987). Briefly, the samples were loaded on SDS-polyacrylamide gel slabs in Tris HCl buffer (0.037 mol/L pH 8.8) and electrophoresed at 100 V (50 mA) for approximately 3 h. The gels were then stained and stored in 7% acetic acid before photography.…”