The preparation of functionalized graphene oxide for targeted intracellular delivery of siRNA

Yang, Xiaoying; Niu, Gaoli; Cao, Xiufen; Yuan, Wen; Xiang, Rong; Duan, Hong‐Quan; Chen, Yongsheng

doi:10.1039/c2jm14718a

Cited by 100 publications

(53 citation statements)

References 27 publications

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“…L. Zhang et al [163] prepared amide derivative of GO with polyethyleniminom in order to load small interfering RNA and doxorubicin. X. Yang et al [215] revealed similar results with polyethylene glycol and 1-pyrenemethylamine-functionalized GO. After further conjugation with folic acid, the complexes were able to selectively deliver small interfering RNA to cells and could effectively knockdown the targeted gene.…”

Section: Gene Delivery By Nanographenesupporting

confidence: 63%

“…By derivatization of carboxylic groups on GO with polyethylenimine, chitosan or 1-pyrenemethylamine, amidic bonds were established [214,162] that allow electrostatic [188] or non-polar π-π stacking interactions [215] and show effi cacy with plasmid DNA or small interfering RNA delivery. Functionalized GO could have effective delivery of molecular beacons [216] and aptamers [217] to cells for in situ specifi c molecule detection.…”

Section: Gene Delivery By Nanographenementioning

confidence: 99%

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Bioimpact of Carbon Nanomaterials

Djordjević

Injac

Mrdjanović

et al. 2013

Advanced Carbon Materials and Technology

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The unique size-dependent properties of carbon nanomaterials (CNMs)-graphene, nanotubes (CNTs) and fullerenes-make them very attractive for diagnostic and therapeutic application. This chapter presents possible application of CNMs. Graphene with extraordinary chemical and physical properties has already revealed a great number of potential applications such as environmental toxic material removal, drug delivery, tissue engineering, and fl uorescence-based biomolecular sensing. The CNT derivatives have many interesting properties which make them potentially useful in a living system as biosensors, bioelectronic devices based on enzyme-nanotube or antibody-nanotube conjugates, chemotherapeutic agents, hyperthermia therapy and immunotherapy agents, agent in treatment of central nervous system disorders, and tissue engineering agent. Fullerene C 60 derivatives have been used as: drug and gene delivery vectors, magnetic resonance imaging agents, radio protectors, antioxidants, HIV-1 protease inhibitors, antigenotoxic agents, and phototherapy agents. Fullerenols are polyhydroxylated derivatives of fullerene (C 60 (OH)n) with remarkable antioxidant, xenobiotic-protective, radioprotective, nanodrug and endohedral gadolinium carrier properties.

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Section: Gene Delivery By Nanographenesupporting

confidence: 63%

Section: Gene Delivery By Nanographenementioning

confidence: 99%

Bioimpact of Carbon Nanomaterials

Djordjević

Injac

Mrdjanović

et al. 2013

Advanced Carbon Materials and Technology

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show abstract

“…1–6 However, applications of graphene in the fields of bioimaging and optoelectronics are limited because graphene is a zero-bandgap semiconductor. 7, 8 In order to generate the bandgap in graphenes, a zero-dimensional derivative, graphene quantum dots (GQDs) have been developed.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of highly fluorescent graphene quantum dots using l-glutamic acid for in vitro/in vivo imaging and sensing

Wu¹,

Tian²,

Wang³

et al. 2013

J. Mater. Chem. C

398

275

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A facile bottom-up method for the synthesis of highly fluorescent graphene quantum dots (GQDs) has been developed using a one-step pyrolysis of a natural amino acid, L-glutamic acid, with the assistance of a simple heating mantle device. The developed GQDs showed strong blue, green and red luminescence under the irradiation of ultra-violet, blue and green light, respectively. Moreover, the GQDs emitted near-infrared (NIR) fluorescence in the range of 800–850 nm with the excitation-dependent manner. This NIR fluorescence has a large Stokes shift of 455 nm, providing significant advantage for sensitive determination and imaging of biological targets. The fluorescence properties of the GQDs, such as quantum yields, fluorescence life time, and photostability, were measured and the fluorescence quantum yield was as high as 54.5 %. The morphology and composites of the GQDs were characterized using TEM, SEM, EDS, and FT-IR. The feasibility of using the GQDs as a fluorescent biomarker was investigated through in vitro and in vivo fluorescence imaging. The results showed that the GQDs could be a promising candidate for bioimaging. Most importantly, compared to the traditional quantum dots (QDs), the GQDs is chemically inert. Thus, the potential toxicity of the intrinsic heavy metal in the traditional QDs would not be a concern for GQDs. In addition, the GQDs possessed an intrinsic peroxidase-like catalytic activity that was similar to the graphene sheets and carbon nanotubes. Coupled with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), the GQDs can be used for the sensitive detection of hydrogen peroxide with a limit of detection of 20 μM.

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“…Sequential delivery of Bcl-2 siRNA and doxorubicin into cancer cells by the GO-PEI conjugate showed signifi cantly improved cell killing effi cacy via a synergistic effect. Yang and co-workers [ 140 ] prepared functionalized GO for the targeted intracellular delivery of hTERT siRNA by conjugating GO with PEG and FA. It could target the HeLa in vitro and the transfected hTERT siRNA could knockdown the protein expression level and mRNA level effi ciently.…”

Section: Image Agentsmentioning

confidence: 99%

New Horizons for Diagnostics and Therapeutic Applications of Graphene and Graphene Oxide

2012

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Graphene, a one-atom-thick two-dimensional (2D) layer of sp(2) -bonded carbon, has received worldwide attention owing to its extraordinary physical and chemical properties. Recently, great efforts have been devoted to explore potential applications of graphene and its oxide in life science, especially in disease-related diagnostics, near-Infrared (NIR) phototherapy and imaging. Here we will introduce recent advances and new horizons in this area, and focus on the rising progress on NIR photothermal therapy for cancer and Alzheimer's disease (AD), human telomerase detection, stem cell proliferation and differentiation on graphene substrate, diagnosis of cancer cell and related biomarkers, drug/nucleotide/peptide delivery and cell imaging, which have not been comprehensively reviewed. We hope to provide an outlook to the applications of graphene and its oxide, especially on the new horizons in this field, and inspire broader interests across various disciplines.

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The preparation of functionalized graphene oxide for targeted intracellular delivery of siRNA

Cited by 100 publications

References 27 publications

Bioimpact of Carbon Nanomaterials

Bioimpact of Carbon Nanomaterials

Fabrication of highly fluorescent graphene quantum dots using l-glutamic acid for in vitro/in vivo imaging and sensing

New Horizons for Diagnostics and Therapeutic Applications of Graphene and Graphene Oxide

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