The preparation of rat’s acellular spinal cord scaffold and co-culture with rat’s spinal cord neuron in vitro

Abstract

The structure of ASCS remained intact, and the neurons were closely arranged in the scaffolds. These results may lay a solid foundation for clinical treatment of SCI when considering glial scar replacement by biomaterials.

A novel method for constructing an acellular 3D biomatrix from bovine spinal cord for neural tissue engineering applications

“…Agarose gel electrophoresis showed that no DNA band was detected in the 3D‐dCBS whereas DNA bands with the high fragment length were detected in the native BSC tissue (Figure b). Thus can be said that we have effectively decellularized 3D‐CBS based upon the findings of studies reported by Crapo et al and Ban et al…”

“…On the other hand, synthetic polymer materials such as polylactic acid, polyglycolic acid, and their copolymers have good degradation speed and biomechanical strength. But there are still difficulties to achieve 3D bioscaffolds by imitating complex biological spinal cord under the current technical circumstances using such polymers . The inadequacy of specific niches and organ‐specific structure in such materials for each cell type is also another crucial disadvantage.…”

“…But there are still difficulties to achieve 3D bioscaffolds by imitating complex biological spinal cord under the current technical circumstances using such polymers. 15 The inadequacy of specific niches and organ-specific structure in such materials for each cell type is also another crucial disadvantage. The potential of acellular biologic scaffolds has been successfully tested prior to clinical studies in various studies in vitro.…”

“…we have effectively decellularized 3D-CBS based upon the findings of studies reported by Crapo et al and Ban et al15,Evaluation of ECM components before and after the decellularization process ECM of CNS acts as a supportive framework which influences cell migration and axonal guidance for spinal cord repair. This structure is primarily supported by a unique ECM framework which consists of collagen, laminin, and fibronectin.…”

Multifunctional biomimetic spinal cord: New approach to repair spinal cord injuries

“…The repair effect is similar to autologous transplantation in 6 mo after surgery[ 19 ]. Ban et al[ 20 ] frozen and thawed the spinal cord tissue, then prepared acellular spinal tissue scaffold by modified chemical extraction. The appearance of the scaffold is comparable to that of the normal spinal cord.…”