The presence ofblaKPC-mediated resistance inEnterobacteriaceaein Cairo University hospitals in Egypt and its correlation within vitrocarbapenem susceptibility

Helal, Soheir Fathy; El-Rachidi, Nevine Gamal El-din; AbdulRahman, Eiman; Hassan, Dina M.

doi:10.1179/1973947813y.0000000099

Cited by 10 publications

(4 citation statements)

References 17 publications

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“…In Africa, K. pneumoniae producing IMP-1 was detected only in Morocco and Tunisia (Chouchani et al, 2011 andBarguigua et al, 2012). Despite bla KPC wasn't detected in our study, other studies showed high prevalence of KPC in Egypt and suggested that it is underestimated problem (Helal et al, 2014 andAbdulalla et al, 2014). However, Djahmi et al (2014) reported that sporadic outbreak or single cases of KPC producers K. pneumoniae in North African countries and the Middle East regions excluding Israel.…”

Section: Resultscontrasting

confidence: 64%

Emergence of New Delhi metallo beta lactamase blaNDM-1 and Oxacillinases blaOXA-48 producing Klebsiella pneumoniae in an Egyptian hospital

Shawky

Tolba

Hamouda

2019

Egypt. J. Microbiol.

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T HE RAPID dissemination of carbapenem resistant Enterobacteriaceae (CRE) all over the world represents a matter of concern. This study aims to investigate the prevalence of CRE in clinical isolates recovered from Al Kasr Al-Ainy hospital in the period between August 2015 and February 2017. The isolates were identified by conventional methods and Maldi-TOF spectroscopy. Phenotypic identification of CRE was carried out by Modified Hodge test and genotypic characterization for Extended-Spectrum Beta-Lactamase (ESBL) and CRE genes was also performed. Carbapenemase activity of CRE isolates was confirmed in 46% of the isolates of which, bla NDM-1 and bla OXA-48 were detected in 75%, 59% of the isolates respectively, while bla VIM was detected in 2.3% only. However, bla IMP and bla KPC were not detected in any isolate. All CRE isolates carried at least one ESBL gene, 95.4% of CRE isolates had bla CTX-M-15 , 88.6% bla TEM-1 and 68.2% bla SHV. The bla SHV gene showed different alleles in the CRE isolates. All the isolates were sensitive to polymyxin B and colistin while only 36.4% were sensitive to tigecycline. Consequently, microbiologists and clinicians should implement the necessary control measures to prevent the spreading of these resistant bacteria.

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Section: Resultscontrasting

confidence: 64%

Emergence of New Delhi metallo beta lactamase blaNDM-1 and Oxacillinases blaOXA-48 producing Klebsiella pneumoniae in an Egyptian hospital

Shawky

Tolba

Hamouda

2019

Egypt. J. Microbiol.

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“…11 The association of both Omp35/36 loss with AmpC production markedly increased the IMI MIC to 8.55 mg/ml, an observation that was also reported by Frazee et al 6 Elevation of IMI MIC within four isolates showed OmpK35/36 loss without production of ESBL or AmpC production was noticed; hence KPC production was tested and confirmed in two isolates. 13 In our study, the mean of ceftazidime inhibition zone diameter was significantly decreased among ESBL-producing isolates showing Omp35 or Omp 36 loss ( p-value 0.041 and OMPK35, OMPK36 PORINS 0.006), respectively. Similarly, it was reported that loss of OmpK35 played a major role in the emergence of ceftazidime resistance.…”

Section: Discussionmentioning

confidence: 82%

The Role of OmpK35, OmpK36 Porins, and Production of β-Lactamases on Imipenem Susceptibility in Klebsiella pneumoniae Clinical Isolates, Cairo, Egypt

Wassef

Abdelhaleim

AbdulRahman

et al. 2015

Microbial Drug Resistance

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“…Figure 6Summary of the prevalence (%) of carbapenemase and ESBL genes in Enterobacteriaceae. Notes: Data from references[52][53][54][55]64,72,93 . a % of ESBL-E; b % of carbapenemase genes; c % of CRE…”

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confidence: 99%

Landscape of Multidrug-Resistant Gram-Negative Infections in Egypt: Survey and Literature Review

El-Kholy¹,

El-Mahallawy²,

Elsharnouby³

et al. 2021

IDR

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This article is the first to review published reports on the prevalence of multidrugresistant (MDR) gram-negative infections in Egypt and gain insights into antimicrobial resistance (AMR) surveillance and susceptibility testing capabilities of Egyptian medical centers. Materials and Methods: A literature review and online survey were conducted. Results: The online survey and literature review reported high prevalence of extendedspectrum β-lactamase (ESBL)-producing Enterobacteriaceae (19-85.24% of E. coli, and 10-87% of K. pneumoniae), carbapenem-resistant Enterobacteriaceae (35-100% of K. pneumoniae and 13.8-100% of E. coli), carbapenem-resistant Acinetobacter baumannii (10-100%), and carbapenem-resistant Pseudomonas aeruginosa (15-70%) in Egypt. Risk factors for MDR Gram-negative infections were ventilated patients (67.4%), prolonged hospitalization (53.5%) and chronic disease (34.9%). Although antimicrobial surveillance capabilities were deemed at least moderate in most centers, lack of access to rapid AMR diagnostics, lack of use of local epidemiological data in treatment decision-making, lack of antimicrobial stewardship (AMS) programs, and lack of risk prediction tools were commonly reported by respondents. Conclusion:This survey has highlighted the presence of knowledge gaps as well as limitations in the surveillance and monitoring capabilities of AMR in Egypt, with most laboratories lacking rapid diagnostics and molecular testing. Future efforts in Egypt should focus on tackling these issues via nationwide initiatives, including understanding the AMR trends in the country, capacity building of laboratories and their staff to correctly and timely identify AMR, and introducing newer antimicrobials for targeting emerging resistance mechanisms in Gram-negative species.

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The presence ofblaKPC-mediated resistance inEnterobacteriaceaein Cairo University hospitals in Egypt and its correlation within vitrocarbapenem susceptibility

Cited by 10 publications

References 17 publications

Emergence of New Delhi metallo beta lactamase blaNDM-1 and Oxacillinases blaOXA-48 producing Klebsiella pneumoniae in an Egyptian hospital

Emergence of New Delhi metallo beta lactamase blaNDM-1 and Oxacillinases blaOXA-48 producing Klebsiella pneumoniae in an Egyptian hospital

The Role of OmpK35, OmpK36 Porins, and Production of β-Lactamases on Imipenem Susceptibility in Klebsiella pneumoniae Clinical Isolates, Cairo, Egypt

Landscape of Multidrug-Resistant Gram-Negative Infections in Egypt: Survey and Literature Review

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