The presence of insulin in and some effects of exogenous insulin on hymenoptera tissues and body fluids

Ishay, Jacob S.; Gitter, S.; Galun, Rachel; Doron, M.; Laron, Zvi

doi:10.1016/s0300-9629(76)80097-7

Cited by 36 publications

(7 citation statements)

References 11 publications

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“…P. americana , Oryctes nasicornis (Bayon 1981) and Aedes aegypti (Brown et aI1985). Similarly, the midgut epithelium of insects also revealed the presence of peptides immunologically related to those of the vertebrate gastrointestinal tissue such as insulin-like peptide in Hymenopteran insects (Ishay et al 1976), glucagon-like peptide' in M anduca sexta (Tager and Kramer 1980) and pancreatic polypeptide-, somatostatin-and enteroglucagon-like immunoreactive materials in P. americana (Iwanaga et aI1981). In fact with an estimated 500 endocrine cells, the midgut of A. aeqypti constituted the largest endocrine organ in an adult mosquito (Brown et al 1985).…”

Section: Discussionmentioning

confidence: 96%

Probable endocrine role of midgut tissue in stimulation of digestive enzyme secretion inOryctes rhinoceros (Coleoptera: Scarabaeidae)

Sreekumar

Prabhu

1988

Proc. Indian Acad. Sci. (Anim. Sci.)

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The effect of midgut epithelial extract on digestive enzyme secretion in the third instar larva of Oryctes rhinoceros was studied. Midguts dissected out from third instar larvae were cut at both ends, emptied of their contents, washed in insect saline, ligated at the open ends and incubated in a medium containing extract of 2 midgut epithelia/lO ml incubation solution. In control experiments midgut preparations were incubated in insect saline without midgut epithelial extract. After 30 min of incubation, the contents of the midgut preparations incubated with midgut epithelial extract showed increased secretion of protease and amylase when compared with controls. Digestive enzyme release into gut lumen appears to be due to the action of a hormone present in the midgut epithelium.

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Section: Discussionmentioning

confidence: 96%

Probable endocrine role of midgut tissue in stimulation of digestive enzyme secretion inOryctes rhinoceros (Coleoptera: Scarabaeidae)

Sreekumar

Prabhu

1988

Proc. Indian Acad. Sci. (Anim. Sci.)

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“…Insulin-like compounds appear to play several roles in insects. These roles include influencing carbohydrate metabolism (Ishay et al, 1976;Duvc et aL, 1979;Moreau et al, 1982;Bounais et aL, 1986), stimulating growth and development (Davis and Shearn, 1977;Mosna, 1981 ), possibly regulating ovarian and embryonic development (Fugo et al, 1987;Saegusa et al, 1992), and acting as prothoracicotropic hormone stimulating the release of ecdysteroids (Ishiz~i and Ichikawa, 1967;Nagasawa et al, 1984;.…”

Section: Until Recently Evidence For Thc Presence Of Neuropeptides Imentioning

confidence: 99%

Localization of insulin-like immunoreactivity in the synganglion of nymphal and adult Dermacentor variabilis (Acari: Ixodidae)

1994

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Immunocytochemical staining based on a peroxidase-antiperoxidase method showed neurosecretory cells (NSC) reactive to bovine insulin in five of 18 paraldehyde fuchsin-positive neurosecretory regions (NSR) in the synganglion of unfed adult Dermacentor variabilis. This is the first report of a neuropeptide in an ixodid tick. The insulin-specific immunoreactive cells included the posterior medial group of the protocerebral center, posterior group of dorsal opisthosomal center, anterior lateral group of the dorso-lateral cheliceral center, dorsal group of the frontal stomodeal center, and anterior group of the ventral palpal center. After feeding and mating, females no longer had immunoreactive cells in three of five NSR found in virgin, unfed females. However, two cells of the posterior group in dorsal opisthosomal center and anterior lateral group of the dorso-lateral cheliceral center remained immunoreactive throughout feeding. Fed, mated males continued to display immunoreactive cells in four of five NSR found in the virgin, unfed males. All developmental stages of nymphs examined had insulin-specific immunoreactive cells in two of the five NSR found in unfed adults, including two positively stained cells of the posterior group in dorsal opisthosomal center and anterior group of ventral palpal neurosecretory center.

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“…Secretion of an insulin-like material in the digestive tract of insects is a subject of dispute. Whereas radioimmunoassays and metabolic bioassays of midgut extracts have indicated the presence of insulin-like protein(s) in several insects [43][44][45][46], the immunohistochemical approach has yielded a positive response in one case only 1391. Differences in the content of midgut hormones among the insect taxa (Table 3) resemble the situation in the vertebrates [47].…”

Section: Discussionmentioning

confidence: 99%

Peptidergic innervation and endocrine cells of insect midgut

Žitňan

Šauman

Sehnal

1993

Arch Insect Biochem Physiol

102

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Antibody against FMRFamide reacts with the stomatogastric innervation and with the midgut endocrine cells in the representatives of most insect orders.The innervation was not revealed in Homoptera, Heteroptera, and Hymenoptera, and the endocrine cells were not recognized in aphids. Other insects exhibited FMRF-amide positive endocrine cells of both open and closed types. The cells are mostly single, rarely grouped, and are distributed unequally in different midgut regions; some of the cells project cytoplasmic extensions indicative of a paracrine function. Investigations on Galleria revealed that the gut innervation persists during midgut reconstruction in the course of metamorphosis. The endocrine cells are sloughed off into the new gut lumen, but there they maintain their antigenic properties until a new population of endocrine cells becomes detectable.Antisera to most mammalian gastroenteropancreatic peptides react specifically with the innervation and/or the endocrine cells of insect midgut; only antisera to bombesin, neurotensin, secretin, motilin, and insulin failed to react. All insects seem to contain antigens that can be detected with antisera to pancreatic polypeptide, FMRFamide, enkephalins, and vasopressins. Stomatogastric innervation and the endocrine cells of some lepidopterans also possess allatotropinand diuretic hormone-like antigens; stomatogastric ganglia, in particular, a prothoracicotropic hormone-like antigen. 0 1993 WiIey-~iss, Inc.

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The presence of insulin in and some effects of exogenous insulin on hymenoptera tissues and body fluids

Cited by 36 publications

References 11 publications

Probable endocrine role of midgut tissue in stimulation of digestive enzyme secretion inOryctes rhinoceros (Coleoptera: Scarabaeidae)

Probable endocrine role of midgut tissue in stimulation of digestive enzyme secretion inOryctes rhinoceros (Coleoptera: Scarabaeidae)

Localization of insulin-like immunoreactivity in the synganglion of nymphal and adult Dermacentor variabilis (Acari: Ixodidae)

Peptidergic innervation and endocrine cells of insect midgut

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