The Preservation of Blood by Rapid Freezing

Rinfret, A. P.; Cowley, C. W.; Doebbler, G. F.; Schreiner, H. R.

doi:10.1159/000426522

Cited by 8 publications

(5 citation statements)

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“…DMSO has also received considerable attention concerning its ability to serve effectively as a cryophylactic agent in the preservation of various body organs, tissues, and cells (7,(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35), including red blood cells (31)(32)(33)(34)(35).…”

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confidence: 99%

“…One difficulty concerning the use of DMSO as a cryophylactic agent in the preservation of blood for transfusion has been its nature to permeate the erythrocyte along with the subsequent difficulty experienced in its removal from the blood preparative to transfusion (32,34,35). Cellular destruction, resulting in hemolysis, commonly accompanies attempts to rid the cells of DMSO by washing (25,36).…”

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confidence: 99%

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Mechanism of Dimethyl Sulfoxide-Induced Hemolysis

Norred

Ansel

Roth

et al. 1970

Journal of Pharmaceutical Sciences

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confidence: 99%

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confidence: 99%

Mechanism of Dimethyl Sulfoxide-Induced Hemolysis

Norred

Ansel

Roth

et al. 1970

Journal of Pharmaceutical Sciences

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“…By restricting the amount of ice formed, the seeding is prevented from reaching a level destructive to the membrane. This restriction may be further enhanced by PVP adsorbing to the cell surface, not only limiting ice formation in that region but possibly protecting sensitive sites thereon [34].…”

Section: Discussionmentioning

confidence: 99%

The Efficiency of Ethylene Glycol, Trehalose and Polyvinylpyrrolidone for Successful Vitrification of IVF Bovine Embryos.

Saha

Otoi²,

Suzuki

1996

Journal of Reproduction and Development

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In Experiment I, a suitable combination of a vitrification solution (VS) and a rehydration solution (RS) was investigated for efficient cryopreservation of in vitro fertilized (IVF) bovine blastocysts on day 7. The variable of the VS is the concentration of polyvinylpyrrolidone (PVP) and that of the RS is the concentration of trehalose. Embryo development of 79% and hatching of 73% were obtained with VS 4 , 40% ethylene glycol (EG) + 11.3% trehalose + 12% PVP and RS 1 , 0% trehalose combination. In Experiment II, IVF bovine embryos on days 7, 8 and 9 were vitrified in VS 4 and rehydrated in RS 1. The highest proportions of embryos reexpanded (84%) and hatched (68%) during culture when the age was day 7. In Experiment III, day 7 embryos which had been vitrified in VS 4 and warmed in 30 C waterbath were kept in situ inside the straw at 5 C or 20 C for 5 min before rehydration in RS 1. Significantly higher proportions of embryos developed in culture when they were kept at 5 C than at 20 C. Furthermore, 3 out of 5 recipients in which vitrified embryos had been directly transferred gave birth to normal calves. Therefore, day 7 IVF bovine blastocysts can be successfully vitrified in VS 4 and can be directly transferred into an isotonic environment.

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“…The interpretation of their results was that old cells were able t o survive much better than young ones. RINFRET [12] prepared fractions of red cells with different mean cell ages by serial hypotonic haemolysis, and found that the young cells were slightly more resistant t o freezing and thawing.…”

Section: Determinations Of the Relative Specific Activity After Mechamentioning

confidence: 99%

“…but so far the complex nature of the "storage lesions" is not understood. I n the recently described techniques [12] of preserving red cells by rapid freezing to temperatures below -100" C followed by rapid thawing, a variable fraction of the red cells is destroyed; some cells are haemolysed during the procedure, and an additional fraction does not survive the immediate post-transfusion period [9].…”

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confidence: 99%