Background: Elder abuse presents a significant public health concern with implications for overall well-being. As Iran undergoes demographic aging, there is a lack of data regarding the prevalence of elder abuse. Objectives: This study aimed to provide insight into the prevalence of elder abuse in Isfahan, Iran. Methods: A cross-sectional study was conducted in Isfahan in 2021, recruiting 424 individuals aged 60 and above from the "Comprehensive Health Services Centers" through simple random sampling. Demographic information and responses to the Elderly Abuse questionnaire were collected via interviews. Descriptive statistics, including mean and confidence intervals, were utilized, while analytical methods such as independent t-tests, chi-square tests, Spearman and Pearson correlation coefficients, and logistic regression were employed for data analysis. Results: The study revealed a 36% prevalence of elder abuse across at least one category, with authority deprivation (19.6%) and emotional neglect (19.3%) being the most prevalent forms. This prevalence was higher among individuals aged 60 - 69 years (adjusted odds ratio (OR) = 2, CI (1.19 - 3.45), P = 0.01), females (adjusted OR = 2.61, CI (1.30 - 5.25), P = 0.007), retirees (adjusted OR = 4.15, CI (1.59 - 10.92), P = 0.004), and those with a history of chronic illness (adjusted OR = 2.11, CI (1.26 - 3.53), P = 0.004). Conclusions: Elder abuse emerges as a significant public health issue in Isfahan. Given the ongoing aging process nationwide, there is a critical need for interventions aimed at preventing and mitigating this public health concern.