The prevalence of enterotoxigenic E. coli isolated from the stools of children aged 0-10 years with diarrhea in mid-anatolia region, Turkey

İşeri, Latife; Apan, Teoman Zafer; Aksoy, Altan; Koç, Figen; Göçmen, Jülide Sedef; Nuristani, Diba

doi:10.1590/s1517-83822011000100030

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…The overall prevalence of ETEC under 5 years old, in this study was ~19%. The isolation rates of ETEC in our study is similar to previous studies that have reported the prevalence of ETEC in developing countries from Bangladesh, Turkey, Peru, Mexico, Egypt, Argentina, India, Nicaragua, and Tunisia which indicated a rate of 18-38% in children [20][21][22][23][24]. However, the ETEC prevalence in our study was lower than what was reported in a previous study (40.7%) conducted in Zambia [18] using Luminex Magpix GPP panel which uses x-TAG technology.…”

Section: Plos Neglected Tropical Diseasessupporting

confidence: 90%

Field evaluation of a novel, rapid diagnostic assay, and molecular epidemiology of enterotoxigenic E. coli among Zambian children presenting with diarrhea

et al. 2022

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Background Enterotoxigenic Escherichia coli (ETEC) is one of the top aetiologic agents of diarrhea in children under the age of 5 in low-middle income countries (LMICs). The lack of point of care diagnostic tools for routine ETEC diagnosis results in limited data regarding the actual burden and epidemiology in the endemic areas. We evaluated performance of the novel Rapid LAMP based Diagnostic Test (RLDT) for detection of ETEC in stool as a point of care diagnostic assay in a resource-limited setting. Methods We conducted a cross-sectional study of 324 randomly selected stool samples from children under 5 presenting with moderate to severe diarrhea (MSD). The samples were collected between November 2012 to September 2013 at selected health facilities in Zambia. The RLDT was evaluated by targeting three ETEC toxin genes [heat labile toxin (LT) and heat stable toxins (STh and STp)]. Quantitative PCR was used as the “gold standard” to evaluate the diagnostic sensitivity and specificity of RLDT for detection of ETEC. We additionally described the prevalence and seasonality of ETEC. Results The study included 324 participants, 50.6% of which were female. The overall prevalence of ETEC was 19.8% by qPCR and 19.4% by RLDT. The children between 12 to 59 months had the highest prevalence of 22%. The study determined ETEC toxin distribution was LT 28/321(9%), ST 18/321(6%) and LT/ST 16/321(5%). The sensitivity and specificity of the RLDT compared to qPCR using a Ct 35 as the cut-off, were 90.7% and 97.5% for LT, 85.2% and 99.3% for STh and 100% and 99.7% for STp, respectively. Conclusion The results of this study suggest that RLDT is sufficiently sensitive and specific and easy to implement in the endemic countries. Being rapid and simple, the RLDT also presents as an attractive tool for point-of-care testing at the health facilities and laboratories in the resource-limited settings.

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Section: Plos Neglected Tropical Diseasessupporting

confidence: 90%

Field evaluation of a novel, rapid diagnostic assay, and molecular epidemiology of enterotoxigenic E. coli among Zambian children presenting with diarrhea

et al. 2022

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“…The overall prevalence of ETEC under 5 years old, in this study was ~19%. The isolation rates of ETEC in our study is similar to previous studies that have reported the prevalence of ETEC in developing countries from Bangladesh, Turkey, Peru, Mexico, Egypt, Argentina, India, Nicaragua, and Tunisia which indicated a rate of 18-38% in children [22][23][24][25][26]. However, the ETEC prevalence in our study was lower than what was reported in a previous study (40.7%) conducted in Zambia [18] using Luminex Magpix GPP panel which uses x-TAG technology.…”

Section: Discussionsupporting

confidence: 90%

Field evaluation of a novel, rapid diagnostic assay RLDT, and molecular epidemiology of enterotoxigenicE. coliamong Zambian children presenting with diarrhea

Silwamba

Chilyabanyama

Liswaniso

et al. 2022

Preprint

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Background Enterotoxigenic Escherichia coli (ETEC) is one of the top aetiologic agents of diarrhea in children under the age of 5 in low-middle income countries (LMICs). The lack of point of care diagnostic tools for routine ETEC diagnosis results in limited data regarding the actual burden and epidemiology in the endemic areas. We evaluated performance of the novel Rapid LAMP based Diagnostic Test (RLDT) for detection of ETEC in stool as a point of care diagnostic assay in a resource-limited setting. Methods We conducted a cross-sectional study of 324 randomly selected stool samples from children under 5 presenting with moderate to severe diarrhea (MSD). The samples were collected between November 2012 to September 2013 at selected health facilities in Zambia. The RLDT was evaluated by targeting three ETEC toxin genes [heat labile toxin (LT) and heat stable toxins (STh, and STp)]. Quantitative PCR was used as the “gold standard” to evaluate the diagnostic sensitivity and specificity of RLDT for detection of ETEC. We additionally described the prevalence and seasonality of ETEC. Results The study included 50.6% of participants that were female. The overall prevalence of ETEC was 19.8% by qPCR and 19.4% by RLDT. The children between 12 to 59 months had the highest prevalence of 22%. The study determined ETEC toxin distribution was LT (49%), ST (34%) and LT/ST (16%). The sensitivity and specificity of the RLDT compared to qPCR using a Ct 35 as the cutoff, were 90.7% and 97.5% for LT, 85.2% and 99.3% for STh and 100% and 99.7% for STp, respectively. Conclusion The results of this study suggest that RLDT is sufficiently sensitive and specific and easy to implement in the endemic countries. Being rapid and simple, the RLDT also presents as an attractive tool for point-of-care testing at the health facilities and laboratories in the resource-limited settings.

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“…Heat-stable and heat-labile enterotoxins encoded by heat-stable enterotoxin (estA) and heat-labile enterotoxin (eltA) genes, respectively, are responsible. These genes can be easily detected by serological assays [39] and multiplex DEC PCR.…”

Section: Common E Coli Pathotypesmentioning

confidence: 99%

Isolation and Characterization of <i>Escherichia coli</i> from Animals, Humans, and Environment

Lupindu¹

2017

≪i>Escherichia Coli

26
1
15
0

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Working on a diverse species of bacteria that have hundreds of pathotypes representing hundreds of strains and many closely related family members is a challenge. Appropriate research design is required not only to achieve valid desired outcome but also to minimize the use of resources, including time to outcome and intervention. This chapter outlines basics of Escherichia coli isolation and characterization strategies that can assist in research designing that matches the set objectives. Types of samples to be collected, collection and storage strategies, and processing of samples are described. Different approaches to isolation, confirmation and concentration of various E. coli strains are summarized in this chapter. Characterization and typing of E. coli isolates by biochemical, serological, and molecular methods have been explained so that an appropriate choice is made to suite a specific E. coli strain/pathotype. Some clues on sample and isolate preservation for future use are outlined, and general precautions regarding E. coli handling are also presented to the researcher to avoid improper planning and execution of E. coli-related research. Given different options, the best E. coli research design, however, should try as much as possible to shorten the length of time to outcomes.

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The prevalence of enterotoxigenic E. coli isolated from the stools of children aged 0-10 years with diarrhea in mid-anatolia region, Turkey

Cited by 10 publications

References 12 publications

Field evaluation of a novel, rapid diagnostic assay, and molecular epidemiology of enterotoxigenic E. coli among Zambian children presenting with diarrhea

Field evaluation of a novel, rapid diagnostic assay, and molecular epidemiology of enterotoxigenic E. coli among Zambian children presenting with diarrhea

Field evaluation of a novel, rapid diagnostic assay RLDT, and molecular epidemiology of enterotoxigenicE. coliamong Zambian children presenting with diarrhea

Isolation and Characterization of <i>Escherichia coli</i> from Animals, Humans, and Environment

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