The Prevalence of ESBL-Producing Strains of E. coli and K. pneumoniae, Isolated from Pets Treated with Antibiotics – Preliminary Remarks

Cozma, A. P.; Carp-Cărare, C.; Porea, Daniela; Mareș, Mihai; Guguianu, E.; Rîmbu, C.; Carp-Cărare, M.

doi:10.15835/buasvmcn-vm:11602

Cited by 3 publications

(7 citation statements)

References 6 publications

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“…It is also higher than 9, 28.9, 2 and 3.6% ESBLresistant/producing enterobacterial isolation prevalence in 22 household dogs in Sweden, 58 faecal samples of healthy dogs, among clinical enterobacterial isolates from 65 dogs/cats/horses and 608 dogs/cats reported by Ljungquist et al (2016), Cozma et al (2015a), Dierikx et al (2012) and Bogaerts et al (2015) in Sweden, Romania, The Netherlands and Europe, respectively. The result is however lower than 62.1 and 30% ESBL-producing enterobacterial isolation prevalence in faecal samples of 29 healthy dogs/cats and among 110 dogs/cats/others reported by Cozma et al (2015b) and Poirel et al (2013) in Romania and France, respectively. A longitudinal study conducted in the Netherlands, reported 45-63% ESBL-producing enterobacterial isolation prevalence in 25 faecal samples of 38 dogs (Baede et al, 2015); this result is also higher than the ESBL-resistant enterobacteria prevalence (27%) recorded in the present study.…”

Section: Discussioncontrasting

confidence: 56%

“…Resistance to extended-spectrum β-lactams in enterobacteria is mediated mainly by extended-spectrum β-lactamases (ESBLs) (Tamang et al, 2012;Ljungquist et al, 2016). ESBL-resistant enterobacteria are multidrug-resistant and exhibit resistance to all β-lactams and many classes of antibacterials including 2015a, Cozma et al, 2015b;Baede et al, 2015;Ljungquist et al, 2016), South America (Moreno et al, 2008;Rocha-Gracia et al, 2015;Oleivera et al, 2016;Carvalho et al, 2016), Asia (Sun et al, 2010;So et al, 2012;Tamang et al, 2014) and Australia (Sidjabat et al, 2007). No single report regarding the presence of ESBL-resistant enterobacteria in companion animals in Nigeria, exist in the literature whereas most Nigerian households keep dogs as pets, guard and/or hunting dogs.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

Anyanwu¹,

Ugwu²,

EZEKWELU³

et al. 2017

Not Sci Biol

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This study was conducted to isolate generic extended-spectrum β-lactam-resistant enterobacteria from household dogs in Nigeria, and to determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 100, randomly selected, apparently healthy household dogs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 4 µg/ml of ceftazidime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 27 ESBL-resistant enterobacterial isolates, 40.7% were Escherichia coli, 37% were Klebsiella species, 18.5% were Salmonella species, while 3.7% was Proteus species. Resistance of the E. coli isolates was 81.8% to ampicillin, 27.2% to streptomycin, 54.5% to ciprofloxacin and tetracycline, 45.4% to enrofloxacin, 90.9% to sulphamethoxazole/trimethoprim, 9.1% to amoxicillin/clavulanic acid and 0% resistant to gentamicin. Resistance of the Klebsiella isolates was 80% to ampicillin, 20% to streptomycin and amoxicillin/clavulanic acid, 30% to ciprofloxacin and enrofloxacin, 60% to tetracycline, 90% to sulphamethoxazole/trimethoprim and 10% to gentamicin. Resistance of the Salmonella isolates was 100% to sulphamethoxazole/trimethoprim, 80% to gentamicin and ampicillin, 60% to streptomycin and tetracycline, 20% to amoxicillin/clavulanic acid, and 0% to ciprofloxacin and enrofloxacin. The Proteus isolate was resistant to streptomycin and gentamycin. All the isolates were resistant to ceftazidime and cefotaxime. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 81.8% for E. coli, 70% for Klebsiella and 100% for Salmonella, respectively. This study has shown that household dogs in Nigeria, are colonized by ESBL-resistant Enterobacteriaceae and are potential reservoirs and disseminators of these organisms.

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Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

Anyanwu¹,

Ugwu²,

EZEKWELU³

et al. 2017

Not Sci Biol

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show abstract

“…After the collecting the samples they were inoculated on nutrient broth and incubated at 37°C for 24 h. 100 μl in a liquid medium and they were used for the execution of the ESBL screening on chromogen solid medium Oxoid Brilliance ESBL Agar supplemented with Cefpodoxime (Cozma et al, 2015). The identification and taxonomic framing of the obtained colonies on the previously mentioned environment was achieved on the basis of some minimal biochemical characters by using MIU and TSI medium.…”

Section: Methodsmentioning

confidence: 99%

“…The presence of the ESBL positive Enterobacteriaceae in the bacterial infections encumber the treatment of these not only in the veterinary medicine but also in the human medicine. In the veterinary medicine, Enterobacteriaceae that produce ESBL were isolated not only from the faeces of the pets (dogs and cats) but also from the commercial animals, these being considered a reservoir of bacteria that produce ESBL (Cozma et al, 2015;Măciucă et al, 2015). The calves and cows are exposed to the treatment with antibiotics either in a curative way, or sometimes in a prophylactic purpose.…”

Section: Introductionmentioning

confidence: 99%

The Prevalence of Esbl-Producing Strains of E.coli, Isolated from Calves with Colibacilosis - Preliminary Remarks

Cozma¹,

Crivei²,

Ciocan³

et al. 2017

BUASVMCN-VM

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The animals producing food have become an increasing reservoir of extended spectrum beta-lactamase producing Enterobacteriaceae. The calves and cows are exposed to a greater quantity of antibiotics, but the data concerning the prevalence of ESBL-producing Enterobacteriaceae are not enough, in comparison with other species of animals used for human consumption, such as birds (Hordijk et al., 2013).The aim of this study was to determine the prevalence of ESBL-producing E. coli involved in some episodes of colibacilosis in calves. Faeces samples were collected from 33 calves with the age ranging between 1-2 weeks and that presented clinical signs of colibacilosis. The samples were collected in a sterile medium for the taxonomic isolation and identification of the etiological agent involved, the ESBL screening being conducted subsequently using the ESBL Agar Oxoid Brilliance chromogenic medium. The phenotypic confirmation of the ESBL-producing strains was conducted in accordance with the CLSI (2014) standard through the combined disc method. Following the tests conducted, out of the 33 strains of isolated E. coli, 9 (27, 27%) were phenotypically confirmed as being ESBL strains.The studies that were previously conducted on the dairy farms have pointed out that the young calves rapidly acquire bacterial strains resistant to antibiotics that are often ESBL strains (Hordijk et al., 2013). The prevalence obtained by us, as well as an insufficient quantity of information concerning the antimicrobial resistance on this segment of species of animals used for the human consumption, support conducting a more thorough study, as well as the identification of ESBL resistance genes, but also of the plasmids that encode the transmission of these genes.

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“…The samples were cultured and incubated at 37 o C for 24 hours (Măciucă, 2014). A volume of 100μl was taken from the liquid which was subsequently streaked out on the Agar Oxoid ESBL Brilliance solid chromogenic medium (Cozma, 2015). For the phenotypic confirmation of the isolated ESBL strains, we used the combined disk test (CLSI, 2014) and the Oxoid Brilliance chromogenic ESBL Agar medium.…”

Section: Methodsmentioning

confidence: 99%

Preliminary Remarks Regarding the Prevalence of ESBL-Producing Strains of E. coli and K. Pneumoniae, Isolated from Cows with Clinical Endometritis

Crivei¹,

Cozma²,

Ciocan³

et al. 2017

BUASVMCN-VM

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ESBL-producing organisms pose unique challenges to clinical microbiologists, clinicians, infection control professionals and antibacterial-discovery scientists. Although the prevalence of ESBLs is not known, it is clearly increasing, and in many parts of the world, 10-40% of strains of E. coli and K. pneumoniae express ESBLs (Rupp and Fey, 2003).The aim of this study was to assess the prevalence of ESBL-positive strains of E. coli and K. pneumoniae in cows with clinical signs of endometritis that were treated exclusively with Oxytetracicline for both diseases of the genital area as well as other bacterial infectious diseases.The study population included 35 Romanian Black Pied cows with clinical signs of endometritis within a farm in North Eastern of Romania. The samples were harvested using sterile cotton swabs that have been further microbiologically processed. For the phenotypic confirmation of the isolated ESBL strains, were used the combined disk test (CLSI, 2014) and the Oxoid Brilliance chromogenic ESBL Agar medium. The taxonomic classification of the isolated colonies was carried out by testing some minimal biochemical characteristics by using the MIU and TSI tests.A total of 47 bacterial strains were isolated from uterine secretions, derived from the 35 cows included in this trial. From the total of 47 isolated bacterial strains, 17 belonged to E. coli and K. pneumoniae species, from which, 6 of them were confirmed as being ESBL-positive.In this preliminary study, by phenotypic methods was confirmed a prevalence of 35.3% for the ESBL strains of E. coli and K. pneumoniae, which requires further research to confirm by molecular biology the identification of ESBL resistance genes, but also for the plasmids encoding these gene transmission.

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The Prevalence of ESBL-Producing Strains of E. coli and K. pneumoniae, Isolated from Pets Treated with Antibiotics – Preliminary Remarks

Cited by 3 publications

References 6 publications

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

The Prevalence of Esbl-Producing Strains of E.coli, Isolated from Calves with Colibacilosis - Preliminary Remarks

Preliminary Remarks Regarding the Prevalence of ESBL-Producing Strains of E. coli and K. Pneumoniae, Isolated from Cows with Clinical Endometritis

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