Caprine arthritis encephalitis is an important disease of dairy goats with a worldwide distribution. Despite the eradication programs, this disease is still prevalent and causing huge financial losses due to reduced lactation performance and culling. The aim of this study was to reveal the presence of CAEV antibodies among a goat population from the North-Eastern of Romania. During 2014-2016, a total of 295 blood samples were collected from healthy or diseased animals. The serum samples were tested for CAEV antibodies detection, using the agar gel immunodiffusion (AGID) test. Within the 295 serum samples tested by AGID technique, 31.86% were confirmed as being positive. All positive samples came from subjects without clinical signs of disease. The results obtained in this preliminary study; show that CAEV is present in French Alpine goats breed within a farm in North-Eastern of Romania.

The preservation in liquid form, at 17°C for several days is still the most used method of semen preservation in boar. In these conditions, it would be of great value to know the factors that can predict the capacity of an ejaculate to maintain its biological value over preservation period. This study aimed to identify the correlations between some parameters of raw semen, and those of extended semen, stored in liquid form, for three days at 17°C. Over 1000 ejaculates were collected by means of manual method and artificial vagina over a period of two years from 400 mature boars housed in a specialized unit in Germany. The semen was examined immediately after collection (before dilution) as well as after three days of preservation. All the parameters (except the volume) were determined by means of computerized instruments. The examination of raw semen was performed using SQA-Vp, while the stored semen was analyzed using Spermvision version 3.7. All data were processed with IBM SPSS Statistics version 21. The Pearson correlation revealed no strong correlation between any of the raw semen parameters when analysed together with stored semen parameters. However, some moderate or weak correlations were found, for example a positive correlation between motility of raw semen and motility of stored semen (p<0.01), a positive correlation between percentage of morphological normal sperm in raw semen and the motility of stored semen (p<0.01), a negative correlation between the concentration of raw semen and the linearity and wobble coefficient in stored semen (p<0.01) as well as a couple other weak correlations. We conclude that, despite the fact that some correlations were revealed, unfortunately the analyzed parameters of raw semen have low predictive value on the parameters of extended semen after preservation at 17°C, and that the parameters of semen will behave differently from one ejaculate to another.

Ovarian remnant syndrome (ORS) represents the presence of functional ovarian tissue after routine castration of the bitch, caused either by surgeon error or the presence of anomalous ovarian tissue. The piece of ovary that remains after the surgery, may continue to develop and become functional, secreting hormones as estrogen and progesterone which cause signs of pro-estrus or estrus, even pseudocyesis. All those signs cause consternation among owners, who thought that the reproductive activity in their dog will be completely abolished. One mixedbreed, 6 years old bitch, 27.3 kg, spayed 8 months before, was presented with purulent vaginal discharge and mammary lumps, after the animal has been treated by her veterinarian with Proligestone to suppress the persisting estrus signs. Our investigations included vaginal cytology, vaginoscopy, the examination of the mammary chain, ultrasound, radiography, serum chemistry, blood count and histopathological examinations. The ultrasound revealed a filled uterine stump and the presence of a mass caudal the right kidney, which was suspected as an ovarian neoplasm. The examination of the mammary chain revealed masses in right M 3, M4, M5 glands and in the left M2 gland. The bitch underwent surgery, and the results of the histopathological examination were: dysgerminoma, pyometra, and the mammary masses were classified as carcinomas. In this paper we wanted to underline the fact that the Ovarian Remnant Syndrome may imply serious clinical consequences which can even lead to the death of the animal. We recommend as a method of treatment for this condition the surgical removal of the remnant tissue.

Cystic fibrosis (CF) is the most common autosomal recessive genetic disease caused by mutation of the CFTR gene. In Romania, CF disease has an estimated a prevalence of 1 case per 2500 live births. Approximately 50% of children die in early life, so the exact number of CF patients in our country remains unknown as no national patient registry exists. This situation has limited our knowledge about the disease progression. Complicated with P. aeruginosa is giving long progressive diseases and often aggravates morbidity and mortality.The aim of the study was to phenotypically frame the P. aeruginosa strains, according to their antibiotic susceptibility profile in order to administer more precisely the antibiotics and to avoid development of multidrug-resistant strains.After the diagnosis of CF, the patients return to a medical control at every 3 months. The sputum samples are collected from these patients, in order to perform the bacteriological examination and the DST test. This study was performed during 1 year (01.01-31.12.2015), and all the sputum samples were cultivated on usual and on special culture media to establish more precisely the type of isolated bacteria. The confirmation was performed using the API 20 NE biochemical test and classified in resistance phenotypes according to CLSI 2015 standards.The study population was represented by 85 infants with CF, from which we isolated 364 bacterial strains. 10.16% of the total of bacterial strains were represented by P. aeruginosa. A major problem represents the repeated antibiotic treatments, this being favourable for the emergence of resistant strains. Antibiotic resistance is currently one of the most important problems faced by clinicians. (7) Multidrug-resistant (MDR) strains of P. aeruginosa were identified in this study and we highlighted that this strains limits the antimicrobial treatment and is shortening the patient's life. (9)

The early resumption of estrous cycles following calving is important for high reproductive efficiency, but also for the cows which haven't been observed in estrus during the first 60 days after calving, the last ones having a significantly higher risk of being culled than cows that have displayed estrus .The aim of this study was to determine the effect of hormonal treatment on reproduction period in cows, and also to establish if there is any significant difference in some reproductive parameters between cows treated with hormonal medication and cows which resumed ovarian cyclicity before 60 days postpartum.This study was performed in a dairy cow's farm in North-Eastern Moldavia, over a period of 2 consecutive years, on Romanian Black Spotted cows, in order to determine hormonal treatments influence over reproduction period. Hormonal treatments used for the above mentioned diseases were represented by luteolytic agent prostaglandin F2α, one of its potent analogues (Proliz, Biotur, Romania) and by administration of exogenous gonadotrophin releasing hormone (Receptal, Intervet, Romania).We noticed that average reproduction period (52.1 days) and the other breeding intervals that were taken under study (over a two years period), presented different values for cows that have been under hormonal medication treatment, compared to those which resumed ovarian cyclicity before 60 days postpartum (52.7 days).Thus, this preliminary study supports the development of a more detailed study on several dairy cows' farms in order to investigate the answer of different types of hormonal treatments against persistent corpus luteum and cystic ovarian disease.