The Prevalence of <italic>Helicobacter pylori</italic> <italic>babA2, iceA1 </italic> and <italic>iceA2 </italic> Genes and Their Association with Clinical Outcomes in Patients with Chronic Gastritis, Ulcerative Diseases and Non-Ulcer Dyspepsia in South East of Iran

Abstract

Background:Helicobacter pylori virulence factors are important in development of the clinical outcomes. The initial stage of colonization is binding of H. pylori to gastric epithelial cells through the babA protein. Heterogeneity among H. pylori strains in presence and expressing the babA gene may be a factor in the variation of clinical outcomes. Likewise, another recently H. pylori described virulence factor; iceA has been shown to be a marker for ulcerative diseases. Objectives: We investigated the presence of babA2, iceA1 and iceA2 H. pylori virulence factors in patients with clinical outcomes in the southeast of Iran. Patients and Methods: In this study, 63 positive culture samples out of 191 biopsies examined to determine of babA2, iceA1 and iceA2 genes by PCR. DNA extracted from 63 Helicobacter positive specimens including 46 chronic active gastritis, 6 ulcerative diseases and non-ulcer dyspepsia 11. Results: The frequency of the babA2, iceA1 and iceA2 genes in the total isolates were 34 (54%), 14 (22.2%) and 34 (54%), respectively. The association of these virulence factors based on sex and age groups were not statistically significant (P > 0.05). There was a borderline significant association between iceA1 and the clinical outcomes (P = 0.094). Conclusions: Our study showed that the prevalence of babA2, iceA1 and iceA2 virulence factors were lower than the other studies that highlighted the role of the geographic factor.

Prevalence of Helicobacter pylori cagA, babA2, and dupA genotypes andcorrelation with clinical outcome in Malaysian patients with dyspepsia

“…Our study failed to find any substantial associations between babA2 and the clinical outcome. This is in agreement with a previous conducted study (18).…”

“…Our study failed to find any substantial associations between babA2 and the clinical outcome. This is in agreement with a previous conducted study (18). During the present study, 24 (22.9%) dupA-positive H. pylori strains were observed in the patients' biopsy samples.…”

“…Although three bab alleles have been identified (babA1, babA2, and babB), only the babA2 gene product is functional for Leb binding activity (16). Some studies discovered a significant relation between babA2 positive genotypes and the occurrence of peptic ulcer diseases (12), while others failed to find these relationships (17,18).…”

Frequency of H.Pylory BabA2 and Hpa Genes in Patients with Dysphagia

“…Following the binding of H.pylori to the mucosal surfaces of the gastrointestinal tract, the host tissue become exposed to the bacterial enterotoxin. Adhesins, proteins, glycoconjugates and bacterial lipids are involved in the early stages of bacterial colonization (3,12). In Shirazi et al study which was conducted in Tehran, although BabA2 gene frequency in gastric cancer samples was higher than other gastrointestinal diseases, no significant relationship was observed between this gene patients with gastric cancer compared with other diseases.…”