The Preventive Effect of Lactobacillus plantarum ZS62 on DSS‐Induced IBD by Regulating Oxidative Stress and the Immune Response

Abstract

In this study, we used DSS to establish an IBD mouse model to study the preventive effect of Lactobacillus plantarum (L. plantarum) ZS62 on IBD in the context of oxidative stress and the immune response. We assessed the mitigating effect of this strain on IBD mice by examining the length of and histopathological changes in the colon, determining the serum antioxidant index and the levels of inflammatory cytokines, as well as the mRNA and protein expression levels of relevant genes. The study results showed that L. plantarum ZS62 could inhibit colonic atrophy in IBD mice, reduce the degree of colonic damage, downregulate the serum levels of MDA, MPO, IL-1β, IL-6, IL-12, TNF-α, and IFN-γ and the relative mRNA and protein expression of IL-1β, IL-12, TNF-α, COX-2, iNOS, and NF-κB p65 in mouse colon tissues, and upregulate the serum levels of CAT, T-SOD, and IL-10 and the relative mRNA and protein expression of Cu/Zn SOD, Mn SOD, GSH-Px, CAT, IL-10, and IκB-α in colon tissues. In summary, L. plantarum ZS62 exhibited a good preventive effect on DSS-induced IBD by regulating oxidative stress and the immune response.

Probiotics (Lactobacillus plantarum HNU082) Supplementation Relieves Ulcerative Colitis by Affecting Intestinal Barrier Functions, Immunity-Related Gene Expression, Gut Microbiota, and Metabolic Pathways in Mice

“…Mucin-2 (MUC-2) is the mucin secreted by goblet cells, which can form the protective layer of the intestinal mucosa epithelium ( 31 ). The tight junction protein zonula occludens-1 (ZO-1) is an important physical barrier located in the gap between intestinal epithelial cells ( 10 ). Studies have shown that the content of ZO-1 and MUC-2 is reduced in UC, and its structure and function are destroyed, resulting in increased intestinal permeability and harmful substances entering the body, aggravating inflammation.…”

“…Due to the shortcomings of traditional treatments, it is urgent to develop new treatments for UC, among which probiotics, as a substitute for antibiotics, have attracted much attention for regulating gut microbiota to effectively alleviate UC ( 9 ). As one of the main probiotics, Lactobacillus plantarum has the characteristics of regulating the balance of gut microbiota, increasing the adhesion of beneficial bacteria to the intestinal mucosa, inhibiting the adhesion of pathogenic bacteria, and inhibiting the inflammatory reaction ( 10 ). Both animal ( 11 ) and clinical trials ( 12 ) have reported that Lactobacillus plantarum can reduce chronic mucosal inflammation in patients with UC and relieve the occurrence of experimental colitis induced by dextran sulfate sodium (DSS).…”

Alleviation of oxidative stress in pancreatic tissue of hyperglycemic mice by Lactiplantibacillus plantarum SCS4

“…The antioxidant system mainly plays a role in scavenging ROS and includes enzymatic and nonenzymatic systems. Among these, the enzymatic system mainly refers to antioxidant enzymes such as SOD and CAT (Pan et al, 2021). In our experiments, we measured the levels of several antioxidant enzymes that could scavenge ROS, including SOD, CAT, GPx, and GSH.…”

Network pharmacology and molecular docking reveal zedoary turmeric-trisomes in Inflammatory bowel disease with intestinal fibrosis

“…These researchers also found that stigmasterol had greater anti-inflammatory activity than β-sitosterol and inhibited the symptoms of colitis[ 23 ]. In this study, the molecular docking results showed that hederagenin, β-sitosterol, formononetin and stigmasterol might be the core components of zedoary turmeric and trisomes involved in the treatment of intestinal fibrosis and might play an antifibrotic role through immune regulation and anti-inflammatory effects[ 24 ]. According to the synergistic effects of multiple components, we speculate that trisomes regulate immunity and exhibit anti-inflammatory effects in the treatment of IBD intestinal fibrosis, whereas zedoary aids the treatment of IBD intestinal fibrosis and improves body function[ 25 ].…”