The production and partial characterization of a monoclonal IgG antibody specific for moulds belonging to the order Mucorales

Abstract

A monoclonal antibody (mAb) was raised against extracellular polysaccharides from Mucor racemosus after intrasplenic immunization of mice. An indirect ELISA and a dot-blot assay were developed with this mAb. The IgG antibody was found to be very specific for all mould species tested belonging to the order of Mucorales, except species belonging to the genus Mortierella sensu stvicto. No cross-reactions were observed with other moulds or yeasts. The immunoreactivity of the polysaccharides of these moulds with this mAb is based on carbohydrate epitopes, in which fucose residues probably play an important role. The mAb may be suited for specific detection of species of the genera Mucor, Rhizopus, Rhizomucor, Thamnidium, Absidia, Syncepkalastrum and species belonging to the Mortierella isabellina group in food, and possibly for diagnosis of mucormycosis in humans.

Generation of a Highly Reactive Chicken-Derived Single-Chain Variable Fragment against Fusarium verticillioides by Phage Display

“…Different types of antibodies, such as polyclonal antisera [22–25], monoclonal antibodies (mAbs) [26–29] and single-chain variable fragments (scFvs) [30–32] specific to various antigens of fungal pathogens have been generated. These antibodies were not only used for detection of pathogens [22–26,28–31] but also for the inhibition of fungal growth [27] and enhancement of resistance in plants [32–36].…”

“…These antibodies were not only used for detection of pathogens [22–26,28–31] but also for the inhibition of fungal growth [27] and enhancement of resistance in plants [32–36]. To date, only polyclonal antibodies have been developed for immunological detection of F. verticillioides [24,25].…”

Production and specificity of polyclonal antibodies against soluble proteins from the arbuscular mycorrhizal fungus Glomus intraradices

“…Immunochemical methods based upon highly selective binding of antibodies (Ab) to the corresponding antigens have been applied for the identification and systematics of bacteria and fungi (Dewey et al 1991;Breuil et al 1992;De Ruiter et al 1993). Polyclonal antibodies (pAb) against AMF extraradical mycelium and spore material (soluble fractions and wall epitopes) have been used for AMF identification (Sanders et al 1992), ecological studies of AMF (Adwell and Hall 1986;Friese and Allen 1991) and research into AMF taxonomy (Adwell et al 1985).…”