1994
DOI: 10.1002/ps.2780420302
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The production of azadirachtin by in‐vitro tissue cultures of neem, Azadirachta indica

Abstract: Callus produced from leaves of a Ghanaian strain of the neem tree, Azadirachta indica A. Juss has been shown to produce the natural insecticide azadirachtin when grown in a defined medium. The azadirachtin was isolated by standard procedures of solvent partition and column chromatography monitored by supercritical fluid chromatography. Biological activity was monitored with antifeedant tests using the desert locust (Schistocerca gregaria Forsk.). The azadirachtin was identified by chromatography on three indep… Show more

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Cited by 40 publications
(36 citation statements)
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“…1). This was confirmed by chemical analysis that showed a tenfold greater amount of azadirachtin in the hairy root biomass compared to the callus line that had previously been shown to contain 7 ppm azadirachtin (Allan et al 1994). This azadirachtin concentration was approximately 30 times more than that detected in natural, plant roots (Sundaram 1996).…”
Section: Discussionmentioning
confidence: 54%
See 1 more Smart Citation
“…1). This was confirmed by chemical analysis that showed a tenfold greater amount of azadirachtin in the hairy root biomass compared to the callus line that had previously been shown to contain 7 ppm azadirachtin (Allan et al 1994). This azadirachtin concentration was approximately 30 times more than that detected in natural, plant roots (Sundaram 1996).…”
Section: Discussionmentioning
confidence: 54%
“…The majority of research on secondary metabolite production from neem tissue cultures has used undifferentiated cultures (Allan et al 1994;Van der Esch et al 1994;Wewetzer 1998). Quantification of azadirachtin from tissue cultures requires extraction and purification of the limonoids, and reliable analysis has shown low azadirachtin yields of 0.5 µg g -1 and 7 µg g -1 dry weight (DW) callus (Schaaf et al 2000;Allan et al 1994, respectively).…”
Section: Introductionmentioning
confidence: 96%
“…A certain degree of differentiation is required for accumulation of secondary metabolism (Violon et al 1984;Allan and Eeswara 1994;Aniruddha and Srivastava 1997). Artemisinin was produced in differentiated shoot cultures of Artemisia annua but was not detected in callus or cell cultures.…”
Section: Discussionmentioning
confidence: 98%
“…This was the first indication to demonstrate that the expression of the tetranortriterpenoids produced by the neem tree in tissue culture could be under hormonal control. Allan et al (1994) also studied the induction of callus cultures from neem from Ghanian origin from leaf explants and the production of azadirachtin by these callus cultures (0.0007 % of the dry weight) after a six week culture period was reported. The feeding deterrent effect was also tested on the desert locust, Schistocerca gregaria.…”
Section: Secondary Metabolites Production From Neem Plant Tissue Culturementioning
confidence: 98%