The production of coumarin phytoalexins in different plant organs of sunflower (Helianthus annuus L.)

Gutiérrez-Mellado, Mari-Carmen; Edwards, Robert; Tena, Marı́a Teresa; Cabello, F.; Serghini, Khadija; Jorrı́n, Jesús

doi:10.1016/s0176-1617(96)80119-2

Cited by 34 publications

(11 citation statements)

References 16 publications

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“…20, 40 and 60 days after sowing), and in ripe seed hulls and kernels of the most susceptible and the most resistant genotypes. The distribution pattern of individual coumarins seen here in capitula and leaves was similar to the distribution reported in vegetative organs by Gutierrez-Mellado et al, (1996). In all cases, scopolin was the most abundant, with values from 20 to 80 μg g −1 tissue fresh weight in seeds, and 5-35 μg g −1 fresh weight in leaves (Table 1).…”

Section: Coumarin Content In Sunflower Genotypes Differentially Affecsupporting

confidence: 83%

Fungitoxic effect of scopolin and related coumarins on Sclerotinia sclerotiorum. A way to overcome sunflower head rot

Prats

Bazzalo²,

Leόn³

et al. 2006

Euphytica

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The content of coumarins, as probable phytoalexins, was analysed in four sunflower genotypes that ranged in responses to head rot from highly susceptible to highly resistant. Low levels of all coumarins (scopolin, scopoletin and ayapin) were detected in the three most susceptible genotypes irrespective of time after inoculation. However, in the resistant genotype there was a clear time-dependent disease-induced increase of all coumarins that reached a maximum after 10-14 days. Detailed comparison of the most susceptible and the resistant genotype showed that in the resistant but not the susceptible, scopoletin peroxidase activity increased during the course of the experiment. Results confirmed a clear negative correlation between coumarin content and disease symptoms and in particular for scopolin. Furthermore we show for the first time that scopolin is inhibitory to Sclerotinia at similar doses to scopoletin. As scopolin is known to be less phytotoxic than ayapin and scopoletin, its accumulation may well confer head rot resistance with minimal plant damage and might be one of the bases for resistance to Sclerotinia.Abbreviations: EMT, esculetin methyl transferase; SGT, scopoletin glucosyl transferase; SP, scopoletin peroxidase

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Section: Coumarin Content In Sunflower Genotypes Differentially Affecsupporting

confidence: 83%

Fungitoxic effect of scopolin and related coumarins on Sclerotinia sclerotiorum. A way to overcome sunflower head rot

Prats

Bazzalo²,

Leόn³

et al. 2006

Euphytica

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“…IEF PAGE showed a range of peroxidase isoforms with differing expression patterns during the post-harvest period in the cassava storage root (Figure 8). A number of peroxidase isoforms with activity towards scopoletin have been detected in other plants (Gutierrez-Mellado et al, 1996), and further IEF PAGE experiments indicated that at least two cassava root anionic peroxidase isoforms showed activity towards scopoletin (Figure 9). The MecPX2 cDNA clone, which has a predicted pI of 8.9 is likely one of the two cationic peroxidase isoforms observed.…”

Section: Expression and Activity Of Enzymes That Modulate Rosmentioning

confidence: 89%

“…Isoelectric focusing polyacrylamide gel electrophoresis (IEF PAGE) was carried out on broad-pI-range ampholine PAG plates pH 3.5-9.5 (Amersham Pharmacia) with the Multiphor II electrofocusing LKB BROMMA 2117 system (Pharmacia Biotechnology). Peroxidase isoforms with activity towards scopoletin were detected according to Gutierrez-Mellado et al (1996). Peroxidase isoforms were detected with guiacol (H 2 O 2 0.1% v/v, aqueous guiacol 10 mM, in 50 mM phosphate buffer pH 5.2) or aminoantipyrine (50 mM phosphate buffer pH 6, 100 mM DHBS (3,5dichloro-2-hydroxybenzenesulfonic acid), 10 mM 4aminoantipyrine, 100 mM H 2 O 2 ).…”

Section: Protein Extraction and Analysismentioning

confidence: 99%

Oxidative stress responses during cassava post-harvest physiological deterioration

et al. 2004

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A major constraint to the development of cassava (Manihot esculenta Crantz) as a crop to both farmers and processors is its starchy storage roots' rapid post-harvest deterioration, which can render it unpalatable and un-marketable within 24-72 h. An oxidative burst occurs within 15 min of the root being injured, that is followed by the altered regulation of genes, notably for catalase and peroxidase, related to the modulation of reactive oxygen species, and the accumulation of secondary metabolites, some of which show antioxidant properties. The interactions between these enzymes and compounds, in particular peroxidase and the coumarin, scopoletin, are largely confined to the vascular tissues where the visible symptoms of deterioration are observed. These, together with other data, are used to develop a tentative model of some of the principal events involved in the deterioration process.

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“…Total phenolic compounds were determined by using the Folin-Ciocalteu reagent, as reported by Prats et al (2002). Thin layer chromatography (TLC) analysis of the methanolic extract was performed according to Gutiérrez-Mellado et al (1996), by using Silicagel 60 F 254 plates (Merck) and ethyl acetate as the mobile phase. Plates were visualized under UV light (254 nm) lamp in a Vilber Lourmat transiluminator (VL-TCP-20 LC).…”

Section: Phenolic Contentmentioning

confidence: 99%

Benzothiadiazole and BABA improve resistance to Uromyces pisi (Pers.) Wint. in Pisum sativum L. with an enhancement of enzymatic activities and total phenolic content

2010

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Benzothiadiazole (BTH) and DL-β-aminobutyric acid (BABA) induced systemic resistance was investigated in susceptible and resistant pea genotypes against Uromyces pisi. Resistance was characterized by reduced infection frequency mainly due to decreases in appressorium formation, stomatal penetration, growth of infection hyphae and haustorium formation. Changes in β-1,3-glucanase, chitinase, phenylalanine ammonia-lyase and peroxidase activities and in total phenolics content, demonstrate that U. pisi resistance is induced by BTH and BABA treatments at early and late stages of the fungal infection process, but that the chemicals operate via different mechanisms. In fact, our study showed that BTH treatment primed the activity of pathogenesis related-proteins such as β-1,3-glucanase, chitinase and peroxidase in both susceptible and resistant genotypes. On the other hand, BABA treatment did not increase the enzymatic activities in the studied genotypes, but significantly increased their total phenolic contents.

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The production of coumarin phytoalexins in different plant organs of sunflower (Helianthus annuus L.)

Cited by 34 publications

References 16 publications

Fungitoxic effect of scopolin and related coumarins on Sclerotinia sclerotiorum. A way to overcome sunflower head rot

Fungitoxic effect of scopolin and related coumarins on Sclerotinia sclerotiorum. A way to overcome sunflower head rot

Oxidative stress responses during cassava post-harvest physiological deterioration

Benzothiadiazole and BABA improve resistance to Uromyces pisi (Pers.) Wint. in Pisum sativum L. with an enhancement of enzymatic activities and total phenolic content

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