The Production of Diabetes Mellitus in Rabbits With Alloxan

Bailey, C. Cabell; Bailey, Orville T.

doi:10.1001/jama.1943.02840340013004

Cited by 81 publications

(26 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thereafter, Brunschwig et al(1943) and Bailey and Bailey (1943) reported the inducement of a persistent diabetic state in animals. This milestone was soon followed by a wealth of studies and dis-…”

Section: Synopsismentioning

confidence: 99%

Enzyme Histochemistry in Experimental Diabetes Mellitus

Ihara

1966

Endocrinol Japon

View full text Add to dashboard Cite

SYNOPSISReported here, are the observations on various histochemical enzyme reactions in several organs (the liver; the kidney; the cardiac, diaphragm, and femoral muscles; the duodenum; the adrenal gland) of rabbits which had been rendered diabetic for from 3 days up to 1 year or more. Independent of the diabetes-inducing reagents (Alloxan or Dithizone) used, the following similar results were obtained: With SD, LD and DPND (which are related to the Krebs cycle activity) and with such phosphatases as ACPase and ALPase, a distinct activity increase could be seen in the liver, the kidney, and the muscular system; except with SD, however, this increase tended to become indistinct or even slightly reduced when 1, 2, or 4 months had elapsed after the induction of diabetes.With G-6-PD (which is related to HMP cycle activity), a distinct decrease in the liver and the kidney could be seen throughout all the diabetic stages. The changes in Ph-rylase activity ran roughly parallel to those in glycogen content, but contradictory changes were found between activity and content in the lipemic heart. Regarding ATPase activity in the lipemic animals, a heavily stained membrane activity could be noted in the liver, the kidney and the cardiac muscle. The lipemic animals also revealed a remarkable increase in non-specific esterase. On the findings in the adrenals, the activities of G-6-PD, SD, ACPase, and ALPase had all been reduced, particularly in the fasciculata when diabetes had persisted for more than 2 or 3 weeks, and ran fairly parallel with the urinary 17-KS and 17-OHCS contents.In addition to these findings, such interesting phenomena were noted as the tendency for the specific zonation of hepatic enzyme distribution to become obscure, the presence of an intimate relation between the activity changes in the liver and the kidney, and the presence of relatively specific activity distribution patterns in the various organs of rabbits having sustained experimental congenital diabetes for more than 1 year.The specific cytotoxic effects of alloxan on pancreatic islets were first studied by Dunn, Sheehan and McLetchie (1943). Thereafter, Brunschwig et al.(1943) and Bailey and Bailey (1943) reported the inducement of a persistent diabetic state in animals. This milestone was soon followed by a wealth of studies and dis-

show abstract

Section: Synopsismentioning

confidence: 99%

Enzyme Histochemistry in Experimental Diabetes Mellitus

Ihara

1966

Endocrinol Japon

View full text Add to dashboard Cite

show abstract

“…12 Although an orally active botanical substitute for insulin is unlikely, phytochemicals may stimulate insulin biosynthesis and promote insulin action. 13 Many plants with antidiabetic properties probably act in part through their content of fibre, vitamins or mineral content. To this end, plants rich in minerals have been shown to enhance glycaemic control in diabetic patients.…”

Section: Introductionmentioning

confidence: 99%

Hypoglycaemic activity of <i>Commelina africana</i> and <i>Ageratum conyzoides</i> in relation to their mineral composition

Agunbiade¹,

Ojezele²,

Ojezele³

et al. 2012

Af Hlth Sci

View full text Add to dashboard Cite

Background: Many plants with antidiabetic properties probably act in part through their content of fibre, vitamins, bioactive or mineral content Objectives: This study investigated the mineral, proximate, phytochemical compositions and hypoglycaemic effect of Commelina africana and Ageratum conyzoides extracts in diabetic rats, and the likely relationship between this property and the mineral, proximate and phytochemical compositions of the plants . Methods:The plants were subjected to mineral, proximate composition and phytochemical analysis. Attempt was made to see (if any) the relationship between the hypoglycaemic effect and the mineral, proximate compositions and phytochemistry of the plants. Alloxan-induced diabetic animals were administered 500mg/kg body weight aqueous extracts of the plants and glibenclamide as the reference hypoglycaemic agent. Results: Aqueous extract of Ageratum conyzoides reduced fasting blood glucose of experimental animals by 39.1% while Commelina africana reduced the same by 78.0%. Alkaloids, cardenolides, saponins, and tannins were detected in both plants. Anthraquinones was absent in C. africana but a trace of it was detected in A. conyzoides. The hypoglycaemic effect of Commelina africana was comparable with the reference hypoglycaemic agent. Ageratum conyzoides showed comparably weaker hypoglycaemic effect than exhibited by reference hypoglycaemic agent. Comparatively, Commelina africana had higher mineral concentrations (except Na) than Ageratum conyzoides. Conclusions: Plants' extracts minerals (magnesium, potassium and iron) and bioactive components (alkaloids and cardenolides) seemingly enhanced their hypoglycaemic effect. Furthermore, these minerals, alkaloids and cardenolides could be helpful in ameliorating complications of diabetes like hypertension and cardiovascular disease.

show abstract

“…But when a special diet, consisting chiefly of wheat flour, was given to these reco vering animals, the reappearance of urinary sugar was confirmed. (Bailey et al, 1943Izeki, 1960. Also no difference was in the found average of the death rate for the 6-month period between the Tolb treated and untreated groups.…”

Section: Discussionmentioning

confidence: 81%