The production of hypericins in two selected Hypericum perforatum shoot cultures is related to differences in black gland structure

Kornfeld, Ari; Kaufman, Peter B.; Lu, Casey R.; Gibson, Donna M.; Bolling, Steven F.; Warber, Sara; Chang, Soo Chul; Kirakosyan, Ara

doi:10.1016/j.plaphy.2006.12.009

Cited by 46 publications

(29 citation statements)

References 18 publications

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“…Agrobacterium rhizogenes-mediated transformation and hairy root induction has been reported for H. perforatum (Di Guardo et al 2003;Vinterhalter et al 2006;Kornfeld et al 2007;Bertoli et al 2008;Koperdáková et al 2009), H. tomentosum and H. tetrapterum ). Spontaneous shoot regeneration from hairy roots has also been reported (Di Guardo et al 2003;Vinterhalter et al 2006).…”

Section: Biotechnology In Hypericum Essential Oil Improvementmentioning

confidence: 97%

“…In fact, they can be black or red in the presence of higher or lower amounts of hypericin, respectively. Indeed, hypericins have been reported to be present in Hypericum species in which dark glands are present (Cakir et al 2003;Piovan et al 2004;Mártonfi et al 2006;Kornfeld et al 2007), but absent in those that lack dark glands (Nunes et al 2010). Hypericin was first identified in Hypericum species and serves as a chemotaxonomic marker for some of them.…”

Section: Introductionmentioning

confidence: 99%

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Hypericum sp.: essential oil composition and biological activities

2012

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Phytochemical composition of Hypericum genus has been investigated for many years. In the recent past, studies on the essential oils (EO) of this genus have been progressing and many of them have reported interesting biological activities. Variations in the EO composition of Hypericum species influenced by seasonal variation, geographic distribution, phenological cycle and type of the organ in which EO are produced and/or accumulated have also been reported. Although many reviews attributed to the characterization as well as biological activities of H. perforatum crude extracts have been published, no review has been published on the EO composition and biological activities of Hypericum species until recently (Crockett in Nat Prod Commun 5(9): 1493-1506, 2010 Bertoli et al. in Global Sci Books 5:29-47, 2011). In this article, we summarize and update information regarding the composition and biological activities of Hypericum species EO. Based on experimental work carried out in our laboratory we also mention possible biotechnology approaches envisaging EO improvement of some species of the genus.

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Section: Biotechnology In Hypericum Essential Oil Improvementmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Hypericum sp.: essential oil composition and biological activities

2012

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“…For example, under normal conditions, hypericin is produced only in the presence of dark hypericin nodules. Several studies have confirmed a clear positive correlation between the presence of dark cell clusters and hypericin accumulation irrespective of the tissue, genotype, species etc . Roots of H. perforatum plants lack these clusters, where no traces of hypericin were found .…”

Section: Resultsmentioning

confidence: 91%

Elicitation as a tool to improve the profiles of high-value secondary metabolites and pharmacological properties of Hypericum perforatum

Shakya

Marslin

Karthik

et al. 2019

Journal of Pharmacy and Pharmacology

126

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Objectives In this review, we aim at updating the available information on the improvement of the Hypericum perforatum L. (Hypericaceae) phytochemical profile and pharmacological properties via elicitation. Key findings Hypericum perforatum seedlings, shoots, roots, calli and cell suspension cultures were treated with diverse elicitors to induce the formation of secondary metabolites. The extracts of the elicitor-treated plant material containing naphthodianthrones, phloroglucinols, xanthones, flavonoids and other new compounds were quantitatively analysed and tested for their bioactivities. While hypericins were mainly produced in H. perforatum cultures containing dark nodules, namely shoots and seedlings, other classes of compounds such as xanthones, phloroglucinols and flavonoids were formed in all types of cultures. The extracts obtained from elicitor-treated samples generally possessed better bioactivities compared to the extract of control biomass. Summary Although elicitation is an excellent tool for the production of valuable secondary metabolites in H. perforatum cell and tissue cultures, its exploitation is still in its infancy mainly due to the lack of reproducibility and difficulties in scaling up biomass production.

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“…Hypericin content of the other clones was comparable although these clones differed in number and density of glands. Concentration of hypericins depends not only on the number of dark glands, their size and area ) but also on their structure (Kornfeld et al 2007). Moreover, different integration sites of rol genes and activation of their expression can explain differences in hypericin content in transgenic clones.…”

Section: Resultsmentioning

confidence: 99%

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

et al. 2008

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Hairy root-regenerated clones of Hypericum perforatum L. grown in vitro similarly to those successfully adapted to ex vitro conditions showed phenotype features typical for plants transformed with Agrobacterium rhizogenes T-DNA. These included reduced apical dominance, increased branching, dwarfing and reduced fertility. Transgenic clones differed in ability to develop root system as a necessary condition for transfer to the soil. One of the profiling characters, capability of hypericin biosynthesis was altered as well. Dark glands as the sites of hypericin accumulation and/or synthesis exhibited significantly higher densities on both, leaves and petals of transgenic clones comparing to controls. In the genome of transgenic clones, rolABC genes were detected. Both clones harboured similar copy number of individual rol genes. However, copy numbers descended from rolA to rolC gene in both clones.

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The production of hypericins in two selected Hypericum perforatum shoot cultures is related to differences in black gland structure

Cited by 46 publications

References 18 publications

Hypericum sp.: essential oil composition and biological activities

Hypericum sp.: essential oil composition and biological activities

Elicitation as a tool to improve the profiles of high-value secondary metabolites and pharmacological properties of Hypericum perforatum

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

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