The production of laccases by white-rot fungi under solid-state fermentation conditions

Chmelová, Daniela; Legerská, Barbora; Kunstová, Jana; Ondrejovič, Miroslav; Miertuš, Stanislav

doi:10.1007/s11274-021-03207-y

Cited by 36 publications

(18 citation statements)

References 148 publications

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“…By contrast, no strain produced enzymes using MKM. This behavior was likely a consequence of the lignocellulosic waste acting as a support for WRF growth, providing conditions similar to their natural habitat and possibly containing substances that promote the production of the desired enzymes [ 48 , 49 ]. The most likely reason for the increase in their production is the presence of phenols such as ferulic, caffeic, coumaric, and chlorogenic acids [ 50 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

“…Likewise, the presence of phenolic compounds from the OMSW would have induced the production of Lac and MnP [ 60 , 61 ]. Although Lac activity was detected, its low production may be due to the C/N ratio of the OMSW, i.e., 60 ( Table 2 ), since some experimental works suggested that substrates with a C/N ratio of less than 16 are the most suitable condition for Lac production [ 48 ]. Additionally, substrates with a ratio greater than 40 can strongly limit Lac production by WRF [ 57 , 58 ].…”

Section: Resultsmentioning

confidence: 99%

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Use of Anthracophyllum discolor and Stereum hirsutum as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste

Benavides

Pinto-Ibieta

Serrano

et al. 2022

Foods

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This study evaluated the use of the white-rot fungi (WRF) Anthracophyllum discolor and Stereum hirsutum as a biological pretreatment for olive mill solid mill waste (OMSW). The WRF strains proposed were added directly to OMSW. The assays consisted of determining the need to add supplementary nutrients, an exogenous carbon source or use agitation systems, and evaluating WRF growth, enzyme activity, phenolic compound removal and lignin degradation. The highest ligninolytic enzyme activity was found at day 10, reaching 176.7 U/L of manganese-independent peroxidase (MniP) produced by A. discolor, and the highest phenolic removal (more than 80% with both strains) was reached after 24 days of incubation. The confocal laser scanning microscopy analysis (CLSM) confirmed lignin degradation through the drop in lignin relative fluorescence units (RFU) from 3967 for untreated OMSW to 235 and 221 RFU, showing a lignin relative degradation of 94.1% and 94.4% after 24 days of treatment by A. discolor and S. hirsutum, respectively. The results demonstrate for the first time that A. discolor and S. hirsutum were able to degrade lignin and remove phenolic compounds from OMSW using this as the sole substrate without adding other nutrients or using agitation systems. This work indicates that it could be possible to design an in situ pretreatment of the valorization of OMSW, avoiding complex systems or transportation. In this sense, future research under non-sterile conditions is needed to evaluate the competition of WRF with other microorganisms present in the OMSW. The main drawbacks of this work are associated with both the low reaction time and the water addition. However, OMSW is seasonal waste produced in one season per year, being stored for a long time. In terms of water addition, the necessary optimization will be addressed in future research.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Use of Anthracophyllum discolor and Stereum hirsutum as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste

Benavides

Pinto-Ibieta

Serrano

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…Likewise, the presence of phenolic compounds from the OMSW would have induced the production of Lac and MnP [53,54]. Although the Lac activity was detected, its low production may be explained by the C/N ratio of the OMSW, i.e., 60 (Table 4), since some experimental works suggested that substrates with a C/N ratio of less than 16 are the most suitable condition for Lac production [47]. Additionally, substrates with a ratio greater than 40 can strongly limit Lac production by WRF [55,56].…”

Section: (A)mentioning

confidence: 98%

“…By contrast, no strain produced enzymes using MKM. This behavior was likely a consequence of the lignocellulosic waste acting as a support for WRF growth, providing conditions similar to their natural habitat and possibly containing substances that promote the production of the desired enzymes [47,48]. The most likely reason for increasing their production is the presence of phenols such as ferulic, caffeic, coumaric and chlorogenic acids [49,50].…”

Section: A) B)mentioning

confidence: 99%

Toward the Fungi Whole Cell as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste

Benavides¹,

Ibieta²,

Serrano³

et al. 2022

Preprint

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White-rot fungi (WRF) have specific enzymes to degrade lignocellulosic and phenolic compounds. Therefore, their direct application could be an alternative to biodegrade complex lignocellulosic biomass such as olive mill solid waste (OMSW). The aim of this study was to evaluate the capacity of A. discolor and S. hirsutum to grow in OMSW as the sole substrate under static conditions and evaluate the phenolic removal compounds and lignin degradation. The lignolytic enzyme activity was determined, as was the phenolic compound removal. At the same time, lignin degradation and structural changes were evaluated by confocal laser scanning microscopy (CLSM) and scanning electron microscope (SEM), respectively. Both strains were able to grow using OMSW as the sole substrate without adding other nutrients, oxygen and/or agitation. The higher ligninolytic enzyme activity was found at day 8, and the highest phenolic removal (more than 80% with both strains) was reached after 24 days of incubation. The CLSM analysis confirmed lignin degradation through the drop in lignin fluorescence from 3967 for untreated OMSW to 235 and 221 RFU after 24 days of treatment by A. discolor and S. hirsutum respectively. The results indicate that both WRF could be suitable candidates to design an in-situ pretreatment step of OMSW, as long as in future research the WRFs have the same performance in non-sterile conditions.

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“…Studies on the biodegradation of agro-wastes or agro-industrial by-products through the solid-state fermentation or submerged fermentation by P. ostreatus, such as (a) using the deinking sludge as a substrate to produce lignocellulolytic enzymes (Vodovnik et al [136], (b) producing exo-polygalacturonases using pomelo peel powder under submerged fermentation by P. ostreatus [122], and (c) producing laccases by white-rot fungi under solid-state fermentation conditions [137]; 3.…”

Section: Mediamentioning

confidence: 99%

Green Biotechnology of Oyster Mushroom (Pleurotus ostreatus L.): A Sustainable Strategy for Myco-Remediation and Bio-Fermentation

et al. 2022

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The field of biotechnology presents us with a great chance to use many organisms, such as mushrooms, to find suitable solutions for issues that include the accumulation of agro-wastes in the environment. The green biotechnology of mushrooms (Pleurotus ostreatus L.) includes the myco-remediation of polluted soil and water as well as bio-fermentation. The circular economy approach could be effectively achieved by using oyster mushrooms (Pleurotus ostreatus L.), of which the substrate of their cultivation is considered as a vital source for producing biofertilizers, animal feeds, bioenergy, and bio-remediators. Spent mushroom substrate is also considered a crucial source for many applications, including the production of enzymes (e.g., manganese peroxidase, laccase, and lignin peroxidase) and bioethanol. The sustainable management of agro-industrial wastes (e.g., plant-based foods, animal-based foods, and non-food industries) could reduce, reuse and recycle using oyster mushrooms. This review aims to focus on the biotechnological applications of the oyster mushroom (P. ostreatus L.) concerning the field of the myco-remediation of pollutants and the bio-fermentation of agro-industrial wastes as a sustainable approach to environmental protection. This study can open new windows onto the green synthesis of metal-nanoparticles, such as nano-silver, nano-TiO2 and nano-ZnO. More investigations are needed concerning the new biotechnological approaches.

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The production of laccases by white-rot fungi under solid-state fermentation conditions

Cited by 36 publications

References 148 publications

Use of Anthracophyllum discolor and Stereum hirsutum as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste

Use of Anthracophyllum discolor and Stereum hirsutum as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste

Toward the Fungi Whole Cell as a Suitable Strategy for Delignification and Phenolic Removal of Olive Mill Solid Waste

Green Biotechnology of Oyster Mushroom (Pleurotus ostreatus L.): A Sustainable Strategy for Myco-Remediation and Bio-Fermentation

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