The production of stable steady-states in mouse ascites mast cell cultures maintained in the chemostat

Moser, H.; Vecchio, Gianna-Jade Del

doi:10.1007/bf02135954

Cited by 12 publications

(1 citation statement)

References 7 publications

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“…They were also able to study the effect of pH on steady state cell concentration and on the specific utilisation rate of glucose. Moser and Vecchio (1967) were able to obtain stable steady states at low dilution rates using mouse ascites mast cells in chemostat cultures. However, long adjustment periods were necessary before oscillations were totally eliminated.…”

mentioning

confidence: 99%

Two-Stage Chemostat Studies of Hybridoma Growth, Nutrient Utilisation, and Monoclonal Antibody Production

Venables

1993

Animal Cell Technology: Basic &Amp; Applied Aspects

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mentioning

confidence: 99%

Two-Stage Chemostat Studies of Hybridoma Growth, Nutrient Utilisation, and Monoclonal Antibody Production

Venables

1993

Animal Cell Technology: Basic &Amp; Applied Aspects

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A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: Effect of nutrient concentration, dilution rate, and pH

Miller

Blanch

Wilke

2000

Biotechnol. Bioeng.

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Hybridomas are finding increased use for the production of a wide variety of monoclonal antibodies. Understanding the roles of physiological and environmental factors on the growth and metabolism of mammalian cells is a prerequisite for the development of rational scale‐up procedures. An SP2/0‐derived mouse hybridoma has been employed in the present work as a model system for hybridoma suspension culture. In preliminary shake flask studies to determine the effect of glucose and glutaminE, it was found that the specific growth rate, the glucose and glutamine metabolic quotients, and the cumulative specific antibody production rate were independent of glucose concentration over the range commonly employed in cell cultures. Only the specific rate of glutamine uptake was found to depend on glutamine concentration. The cells were grown in continuous culture at constant pH and oxygen concentration at a variety of dilution rates. Specific substrate consumption rates and product formation rates were determined from the steady state concentrations. The specific glucose uptake rate deviated from the maintenance energy model1 at low specific growth rates, probably due to changes in the metabolic pathways of the cells. Antibody production was not growth‐associated; and higher specific antibody production rates were obtained at lower specific growth rates. The effect of pH on the metabolic quotients was also determined. An optimum in viable cell concentration was obtained between pH 7.1 and 7.4. The viable cell number and viability decreased dramatically at pH 6.8. At pH 7.7 the viable cell concentration initially decreased, but then recovered to values typical of pH 7.1–7.4. Higher specific nutrient consumption rates were found at the extreme pH values; however, glucose consumption was inhibited at low pH. The pH history also influenced the behavior at a given pH. Higher antibody metabolic quotients were obtained at the extreme pH values. Together with the effect of specific growth rate, this suggests higher antibody production under environmental or nutritional stress.

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A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: Effect of nutrient concentration, dilution rate, and pH

Miller

Blanch

Wilke

1988

Biotech & Bioengineering

441

102

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Hybridomas are finding increased use for the production of a wide variety of monoclonal antibodies. Understanding the roles of physiological and environmental factors on the growth and metabolism of mammalian cells is a prerequisite for the development of rational scale-up procedures. An SP2/0-derived mouse hybridoma has been employed in the present work as a model system for hybridoma suspension culture. In preliminary shake flask studies to determine the effect of glucose and glutamine, it was found that the specific growth rate, the glucose and glutamine metabolic quotients, and the cumulative specific antibody production rate were independent of glucose concentration over the range commonly employed in cell cultures. Only the specific rate of glutamine uptake was found to depend on glutamine concentration. The cells were grown in continuous culture at constant pH and oxygen concentration at a variety of dilution rates. Specific substrate consumption rates and product formation rates were determined from the steady state concentrations. The specific glucose uptake rate deviated from the maintenance energy model(1) at low specific growth rates, probably due to changes in the metabolic pathways of the cells. Antibody production was not growth-associated; and higher specific antibody production rates were obtained at lower specific growth rates. The effect of pH on the metabolic quotients was also determined. An optimum in viable cell concentration was obtained between pH 7.1 and 7.4. The viable cell number and viability decreased dramatically at pH 6.8. At pH 7.7 the viable cell concentration initially decreased, but then recovered to values typical of pH 7.1-7.4. Higher specific nutrient consumption rates were found at the extreme pH values; however, glucose consumption was inhibited at low pH. The pH history also influenced the behavior at a given pH. Higher antibody metabolic quotients were obtained at the extreme pH values. Together with the effect of specific growth rate, this suggests higher antibody production under environmental or nutritional stress.

show abstract

The production of stable steady-states in mouse ascites mast cell cultures maintained in the chemostat

Cited by 12 publications

References 7 publications

Two-Stage Chemostat Studies of Hybridoma Growth, Nutrient Utilisation, and Monoclonal Antibody Production

Two-Stage Chemostat Studies of Hybridoma Growth, Nutrient Utilisation, and Monoclonal Antibody Production

A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: Effect of nutrient concentration, dilution rate, and pH

A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: Effect of nutrient concentration, dilution rate, and pH

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