The profile of HSP90 gene expression of Bali cattle to heat stress in West Sumbawa, West Nusa Tenggara

Puteri, S. A.; Aritonang, S. B.; Nussa, H. T.; Rahmani, A. F.; Bowolaksono, Anom; Lestari, Retno

doi:10.1063/1.5064135

Cited by 2 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Heat shock proteins (HSPs) are a large family of proteins that are highly conserved in evolutionary lines [ 45 ], and can be seen in the thermal tolerance and adaptation of organism cells to various stresses. These proteins have chaperone activity that ensures the folding, unfolding, and refolding of stress-denatured proteins [ 46 ]. In addition, HSPs play a crucial role in the adaptation of mammals, including cattle, to stressful conditions [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of Heat Stress on Blood, Production, and Physiological Indicators in Heat-Tolerant and Heat-Sensitive Dairy Cows

Chen

Shu

Sun

et al. 2023

Animals

View full text Add to dashboard Cite

Heat stress affects production and health in cows severely. Since it is difficult to define heat-tolerant animals, studies of response to heat stress are important for understanding dairy cows’ health and production. However, information on the impact of heat stress on various indicators in heat-tolerant and heat-sensitive cows is sparse. This study aimed to investigate the effects of heat stress (HS) on blood, production, and physiological indicators in heat-tolerant and heat-sensitive cows. A total of 43 dairy cows were used from 9 May to 7 August 2021, under Temperature–Humidity Index (THI) measurements that ranged from 65.9 to 86.7. We identified cows that were tolerant or sensitive to HS based on the slope of the response of physiological and production traits against THI during the HS period by using a clustering method. After HS, serum glucose (Glu), cortisol (COR), 5-hydroxytryptamine (5-HT), and interleukin-6 (IL-6) levels of cows in the heat-tolerant group were lower than in the heat-sensitive group (p < 0.05). With THI as the predictor, the R2 for predicting respiration rate (RR) and body surface temperature (BT) in heat-tolerant cows was 0.15 and 0.16, respectively, whereas the R2 for predicting RR and BT in heat-sensitive cows was 0.19 and 0.18, respectively. There were low to moderate, positive correlations between RR, BT, and MY with THI, with Pearson correlation coefficients ranging from r = 0.11 to 0.4 in the heat-tolerant group, and from r = 0.24 to 0.43 in the heat-sensitive group. There was a low positive correlation between VT and THI, with a Spearman correlation coefficient of r = 0.07 in the heat-sensitive group. The heat-tolerant dairy cows had lower MY losses and had lower MY (p = 0.0007) in mixed models. Heat-tolerant cows with low-stress levels, through upregulating RR rapidly, increased their adaptability to thermal environments. They have better thermoregulation capability; the hypothalamic–pituitary–adrenal (HPA) axis regulated the thermoregulatory in animals by releasing a variety of neurotransmitters and hormones.

show abstract

Section: Discussionmentioning

confidence: 99%

Impact of Heat Stress on Blood, Production, and Physiological Indicators in Heat-Tolerant and Heat-Sensitive Dairy Cows

Chen

Shu

Sun

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

“…Though there are many HSP genes, thermo tolerance in livestock animals is primarily associated with the HSP70 and HSP90 genes. The most common and temperature-sensitive is HSP90, which is thought to play an important role in environmental stress and thermal adaptation (Gade et al, 2010;Aritonang et al, 2017;Puteri et al, 2018). In summer, higher levels of expression of proteins of the HSP70 and HSP90 families have been observed in a variety of livestock species (Archana et al, tolerance, milk production, fertility, and disease susceptibility in livestock (Shergojry et al, 2014a;Kumar et al, 2015;Bhat et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Identification of Polymorphism within Exon 8 of Bovine HSP90AA1 Gene using PCR-SSCP Technique

Prasanna¹,

Rao²,

Prakash³

et al. 2021

IJBSM

View full text Add to dashboard Cite

Heat shock proteins (HSPs) are a type of molecular chaperones that aid in the recovery of stressed cells and serve as a major system for intracellular self-defense. A study was conducted during the year 2018–19 at College of Veterinary Science Rajendranagar, Hyderabad, Telengana State, India to find polymorphisms in exon 8 of the bovine HSP90AA1 gene in Sahiwal (n=50) and crossbred (n=50) cows. Blood samples were collected from the experimental animals and genomic DNA was isolated. Physiological parameters like body temperature and respiration rate for each animal were taken during the experimental period and the heat tolerance coefficient was calculated. The data on production and reproduction traits were obtained from the history sheets of the animals. To detect the polymorphism, a 539 bp fragment of the HSP90AA1 gene covering exon 8 was subjected to the Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique.The PCR-SSCP of exon 8 of HSP90AA1 gene yielded two genotypic patterns AA and AB corresponding to two allelic variants with frequencies of 0.85, 0.15, 0.81 and 0.19 in Sahiwal and crossbred cows, respectively. The PCR-SSCP patterns obtained were correlated with the physiological, productive, and reproductive traits in both Sahiwal and crossbred cows. The association analysis of SSCP patterns of the exon 8 of HSP90AA1 gene revealed non-significant effect in Sahiwal cows, although the AB genotype had a significantly longer service period in crossbred cows.

show abstract

The profile of HSP90 gene expression of Bali cattle to heat stress in West Sumbawa, West Nusa Tenggara

Cited by 2 publications

References 6 publications

Impact of Heat Stress on Blood, Production, and Physiological Indicators in Heat-Tolerant and Heat-Sensitive Dairy Cows

Impact of Heat Stress on Blood, Production, and Physiological Indicators in Heat-Tolerant and Heat-Sensitive Dairy Cows

Identification of Polymorphism within Exon 8 of Bovine HSP90AA1 Gene using PCR-SSCP Technique

Contact Info

Product

Resources

About