The proglycation effect of caffeic acid leads to the elevation of oxidative stress and inflammation in monocytes, macrophages and vascular endothelial cells

Wu, Chi Hao; Huang, Hsiao Wen; Lin, Jer‐An; Huang, Shang Ming; Yen, Gow‐Chin

doi:10.1016/j.jnutbio.2010.05.002

Cited by 37 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wu et al (2011) reported that the constituent C exhibited proglycation effects (or pro-oxidant activity detected by electron spin resonances), which were enhanced by metal ions (such as Fe 2+ and Cu 2+ ) and abolished by EDTA, reduced glutathione, or α-lipoic acid in BSA/MGO models. The present results were similar to those of Gugliucci et al (2009) and different from those of Wu et al (2011). This might be due to the detection method used (antibody detection vs. protein cross-linking in SDS-PGAE gels) and the number of incubation days (3-day vs. 7-day).…”

Section: Resultsmentioning

confidence: 99%

“…(Wu and Yen The BSA/MGO models for protein glycation experiments in vitro 2005;Wu et al 2011) were followed with some modifications. The total 100-μl reaction solution contained 20 μl of BSA solution (2 mg/ml), 10 μl of PBS (10-fold dilutions in the final), 10 μl of 10 mM or 50 mM MGO, 50 μl of tested compounds (A, I, C and D, each 1 mM or 10 mM), and distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…AGE-RAGE interaction may overgenerate intracellular ROS, which retards glucose uptake into adipocytes and then induces monocyte inflammatory factor, chemoattractant protein-1, to participate in the development of obesity-related insulin resistance (Unoki and Yamagishi 2008). Wu et al (2011) reported that the MGO-mediated glycated fetal bovine serum can increase intracellular ROS and IL-1β expressions in THP-1 monocytes, and TNF-α expressions in RAW264.7 macrophages. They proposed that MGO-mediated glycated fetal bovine serum can cause oxidative and inflammatory injury of monocytes, macrophages and vascular endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inhibitory activities of acteoside, isoacteoside, and its structural constituents against protein glycation in vitro

Liu

Han

et al. 2013

Bot Stud

View full text Add to dashboard Cite

BackgroundAdvanced glycation end products (AGE) are substances that can induce insulin resistance in adipocyte, hepatocyte and muscle cells. This resistance correlates highly with cardiovascular disease and diabetic complications. Acteoside (A), a phenylethanoid glycoside, is an active compound in several plants and traditional herbal medicines. Acteoside, its structural isomer, isoacteoside (I), and their constituents, caffeic acid (C) and 3,4-dihydroxyphenylethanol (D), were used in the study to investigate the inhibitory activity against AGE formations in vitro.ResultsAGE formations were detected by anti-(Nϵ-(carboxymethyl)lysine (anti-CML), using bovine serum albumin (BSA)/glucose (glc) and BSA/galactose (gal) as models, or by anti-argpyrimidine (anti-AP), using BSA/methylglyoxal (MGO) as models. It was found that A, I, C, or D, each at 5 mM, could attenuate the CML formations detected by ELISA in the BSA/gal model of a 3-day or 5-day reaction, and showed significant differences (P < 0.01 or P < 0.001) compared to the control. However, these compounds showed a minor effect after a 7-day incubation. It was also found that C or D could lower the CML formations in the BSA/glc model and showed significant differences (P < 0.05 or P < 0.01) compared to the control after a 3-day, 5-day and 7-day reaction. It was found that A, I, C, or D, each at 0.5 mM or 5 mM, could attenuate the AP formations in the BSA/MGO model of a 3-day reaction and showed significant differences (P < 0.001) compared to the control.ConclusionsThe results suggest the potential anti-glycation activities of A and I in vitro may apply to cell models at higher glucose concentrations or to diabetic animal models, and need further investigation.Electronic supplementary materialThe online version of this article (doi:10.1186/1999-3110-54-6) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibitory activities of acteoside, isoacteoside, and its structural constituents against protein glycation in vitro

Liu

Han

et al. 2013

Bot Stud

View full text Add to dashboard Cite

show abstract

“…In the study of Wu et al ( 2011 ), 2′,7′-dichlorofl uorescein (DCF) is used to measure ROS production. The cell-permeant 2′,7′-dichlorodihydrofl uorescein diacetate (H 2 DCFDA) (also known as dichlorofl uorescein diacetate) is a chemically reduced form of fl uorescein used as an indicator for ROS in cells.…”

Section: Read-out Of the Systemmentioning

confidence: 99%

THP-1 and U937 Cells

Chanput

Peters

Wichers

2015

The Impact of Food Bioactives on Health

View full text Add to dashboard Cite

Monocytes are circulatory precursor cells from myeloid origin that can develop into macrophages or dendritic cells upon migration from the blood stream to tissues. Both macrophages and dendritic cells are professional antigen-presenting cells. Monocytes and their macrophage and dendritic-cell progeny serve three main functions in the immune system. These are phagocytosis, antigen presentation, and cytokine production. THP-1 and U937 are (pro-) monocytic cell lines that can, also in vitro, be differentiated into either various types of macrophages or into dendritic cells.This chapter describes how to grow THP-1, resp. U937 cells, how to differentiate these into more specialised phenotypes such as various macrophage types or dendritic cells, how to read-out their responses to stimuli and it gives examples of how such cell lines have been used into studying the effects of food compounds.

show abstract

“…The development of innovative novel therapeutics for the management of HCC is particularly urgent. Accumulating evidence has demonstrated that CaA and ChA possessed various pharmacological effects, such as antioxidative, antiviral, antitumor, and anti-inflammatory activities 24–26 . The aim of this study was to evaluate the effects of CaA and ChA on HCC induced by DEN.…”

Section: Discussionmentioning

confidence: 99%

Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid

Zhang

Wang

Qiao

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) treatment remains lack of effective chemopreventive agents, therefore it is very attractive and urgent to discover novel anti-HCC drugs. In the present study, the effects of chlorogenic acid (ChA) and caffeic acid (CaA) on HCC induced by diethylnitrosamine (DEN) were evaluated. ChA or CaA could reduce the histopathological changes and liver injury markers, such as alanine transarninase, aspartate aminotransferase, alkaline phosphatase, total bile acid, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol. The underlying mechanisms were investigated by a data integration strategy based on correlation analyses of metabonomics data and 16 S rRNA gene sequencing data. ChA or CaA could inhibit the increase of Rumincoccaceae UCG-004 and reduction of Lachnospiraceae incertae sedis, and Prevotella 9 in HCC rats. The principal component analysis and partial least squares discriminant analysis were applied to reveal the metabolic differences among these groups. 28 different metabolites showed a trend to return to normal in both CaA and ChA treatment. Among them, Bilirubin, L-Tyrosine, L-Methionine and Ethanolamine were correlated increased Rumincoccaceae UCG-004 and decreased of Lachnospiraceae incertae sedis and Prevotella 9. These correlations could be identified as metabolic and microbial signatures of HCC onset and potential therapeutic targets.

show abstract

The proglycation effect of caffeic acid leads to the elevation of oxidative stress and inflammation in monocytes, macrophages and vascular endothelial cells

Cited by 37 publications

References 33 publications

Inhibitory activities of acteoside, isoacteoside, and its structural constituents against protein glycation in vitro

Inhibitory activities of acteoside, isoacteoside, and its structural constituents against protein glycation in vitro

THP-1 and U937 Cells

Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid

Contact Info

Product

Resources

About