The prognostic significance of tumor cell detection in intraoperative pleural lavage and lung tissue cultures for patients with lung cancer

Buhr, J; Berghäuser, K. H.; Gonner, S.; Kelm, C; Burkhardt, E.; Padberg, Winfried

doi:10.1016/s0022-5223(97)70225-3

Cited by 62 publications

(40 citation statements)

References 18 publications

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“…In Asian countries, the predominant histological type of esophageal carcinoma is squamous cell carcinoma, and tumors are frequently located in the thoracic esophagus. As in lung cancer (11)(12)(13)(14), the presence of exfoliated cancer cells in the pleural cavity after The results are presented as the means ± SEM. The isotonic NaCl buffer before cell suspension, n=5; the other solutions, n=3.…”

Section: Discussionmentioning

confidence: 99%

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Kosuga

Shiozaki

Ichikawa³

et al. 2011

Oncol Rep

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Abstract. This study aimed to investigate cytocidal effects of hypotonic shock on esophageal squamous cell carcinoma (ESCC) cell lines, and to apply pleural lavage with distilled water to surgery for ESCC. Three human ESCC cell lines, TE5, TE9 and KYSE170 were exposed to distilled water, and morphological changes in ESCC cells were closely observed under a differential interference contrast microscope connected to a high-speed digital video camera. Further, serial cell volume changes after hypotonic shock were measured using a high-resolution flow cytometer. To investigate the cytocidal effects of hypotonic shock on ESCC cells, re-incubation of ESCC cells was performed after hypotonic shock. Additionally, the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB), a Cl -channel blocker, during hypotonic shock were analyzed. Video recordings by high-speed digital camera demonstrated that hypotonic shock with distilled water induced cell swelling followed by cell rupture. Measurements of cell volume changes using a high-resolution flow cytometer indicated that severe hypotonicity with distilled water increased broken fragments of ESCC cells within 5 min. Re-incubation experiments demonstrated cytocidal effects of hypotonic shock on ESCC cells. Treatment of cells with NPPB increased cell volumes by the inhibition of regulatory volume decrease, which is observed during hypotonic shock, and enhanced cytocidal effects. These findings demonstrated the cytocidal effects of hypotonic shock on ESCC cells, and clearly support the efficacy of pleural lavage with distilled water during surgery for ESCC.

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Section: Discussionmentioning

confidence: 99%

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Kosuga

Shiozaki

Ichikawa³

et al. 2011

Oncol Rep

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“…For example, in lung cancer the absence or presence of malignant cells in pleural effusions discriminates limited (e.g., T1) from locally advanced stages (e.g., T4), with very different consequences for therapeutic management. It could be decisive, before a planned resection of lung cancer, to analyse by cytology and FISH an accompanying effusion or a pleural lavage (Buhr et al, 1997). Improved detection of pleural metastasis by 'molecular upstaging' could prevent patients with more extensive disease from undergoing surgery.…”

Section: Discussionmentioning

confidence: 99%

Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)

et al. 2004

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Diagnosis of malignant cells in effusions is important for staging procedures and resulting therapeutic decisions. Cytodiagnostics in effusions is sometimes difficult since reactive mesothelial cells can mimic malignant cells. We used fluorescence in situ hybridisation (FISH) in single-colour or if appropriate in dual-colour evaluation to detect chromosomal aberrations in effusion cells as markers of malignancy, to raise the diagnostic yield. Cytologic and FISH evaluations -by using probes representing several chromosomes always including chromosomes 11 and 17 -were performed in 358 effusion fluids. Cytology was positive for malignancy in 44.4% of all effusions, whereas FISH was positive in 53.9% (P ¼ 0.0001). The combination of cytology and FISH was diagnostic for malignancy in 60.9% of effusions. Diagnostic superiority of FISH was demonstrated in effusions from breast cancer, lung cancer, pancreatic cancer, and in effusions from the entire group of gynaecological and gastrointestinal carcinomas. In transudates (effusion protein o2.5 g dl À1 ), malignant cells were detectable by cytology, FISH, and combined use of both methods in 18.6, 30, and 37.1% of effusions, respectively, suggesting that cytologic and molecular analysis should be performed also with transudates. In conclusion, FISH in combination with conventional cytology is a highly sensitive and specific diagnostic tool for detecting malignant cells in effusions.

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“…Spread of malignancies to the pleural cavity, resulting in malignant pleural effusions and pleural metastatic disease are associated with a dismal prognosis, with most patients surviving only a few months after the diagnosis is made (40)(41)(42)(43)(44)(45)(46)(47). While NSCLC is the most common cause of malignant disease of the pleura, the pleural model used in this study is also relevant to other cancer types, including mesothelioma, breast, ovarian, and lymphoid cancers (48)(49)(50).…”

Section: Discussionmentioning

confidence: 99%

“…Such foci of disease are extremely difficult to diagnose preoperatively. Metastatic disease in the pleural cavity may indicate a more aggressive tumor biology an a higher risk of recurrence (12)(13)(14)(15)(16), which could contribute to the decreased survival often seen in these patients. As such, new techniques are needed to both diagnose and treat metastatic pleural spread of NSCLC.…”

Section: Introductionmentioning

confidence: 99%

Minimally invasive localization of oncolytic herpes simplex viral therapy of metastatic pleural cancer

et al. 2005

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Purpose-Herpes simplex virus-one (HSV-1) oncolytic therapy and gene therapy are promising treatment modalities against cancer. NV1066, one such HSV-1 virus carries a marker gene for enhanced green fluorescent protein (EGFP). The purpose of this study was to determine whether NV1066 is cytotoxic to lung cancer and whether EGFP is a detectable marker of viral infection in vitro and in vivo. We further investigated whether EGFP expression in infected cells can be used to localize the virus and to identify small metastatic tumor foci (< 1 mm.) in vivo by means of minimally invasive endoscopic systems equipped with fluorescent filters.Experimental Design-In A549 human lung cancer cells, in vitro viral replication was determined by plaque assay, cell kill by LDH release assay, and EGFP expression by flow cytometry. In vivo, A549 cells were injected into the pleural cavity of athymic mice. Mice were treated with intrapleural injection of NV1066 or saline and examined for EGFP expression in tumor deposits using a stereomicroscope or a fluorescent thoracoscopic system. Results-NV1066 replicated in, expressed EGFP in infected cells and killed tumor cells in vitro.In vivo, treatment with intrapleural NV1066 decreased pleural disease burden, as measured by chest wall nodule counts and organ weights. EGFP was easily visualized in tumor deposits, including microscopic foci, by fluorescent thoracoscopy.Conclusions-NV1066 has significant oncolytic activity against a human NSCLC cell line and is effective in limiting the progression of metastatic disease in an in vivo orthotopic model. By incorporating fluorescent filters into endoscopic systems, a minimally-invasive means for diagnosing small metastatic pleural deposits and localization of viral therapy for thoracic malignancies may be developed using the EGFP marker gene inserted in oncolytic herpes simplex viruses.

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The prognostic significance of tumor cell detection in intraoperative pleural lavage and lung tissue cultures for patients with lung cancer

Abstract: The positive result in pleural lavage seems to be a prognostic predictor for patients with lung cancer.

Cited by 62 publications

References 18 publications

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)

Minimally invasive localization of oncolytic herpes simplex viral therapy of metastatic pleural cancer

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